Cargando…
HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis
Background: Differentiating metastatic cervical cancer from another primary tumor can be difficult in patients with a history of cervical cancer and a distant lesion. The use of routine HPV molecular detection and genotyping tests could help in these cases. The objective of this study was to identif...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961249/ https://www.ncbi.nlm.nih.gov/pubmed/36836411 http://dx.doi.org/10.3390/jpm13020177 |
_version_ | 1784895708040527872 |
---|---|
author | Montero-Macías, Rosa Coronado, Pluvio J. Robillard, Nicolas Veyer, David Villefranque, Vincent Le Frére-Belda, Marie-Aude Auberger, Elisabeth Bitolog, Pauline Stankovic, Ivana Bélec, Laurent Bats, Anne-Sophie Lécuru, Fabrice Péré, Hélène |
author_facet | Montero-Macías, Rosa Coronado, Pluvio J. Robillard, Nicolas Veyer, David Villefranque, Vincent Le Frére-Belda, Marie-Aude Auberger, Elisabeth Bitolog, Pauline Stankovic, Ivana Bélec, Laurent Bats, Anne-Sophie Lécuru, Fabrice Péré, Hélène |
author_sort | Montero-Macías, Rosa |
collection | PubMed |
description | Background: Differentiating metastatic cervical cancer from another primary tumor can be difficult in patients with a history of cervical cancer and a distant lesion. The use of routine HPV molecular detection and genotyping tests could help in these cases. The objective of this study was to identify if an easy-to-use HPV molecular genotyping assay would allow differentiating between HPV tumor metastasis and a new independent primary non-HPV-induced tumor. Materials and Methods: Between 2010 and 2020, we identified patients with a primary cervical carcinoma who also had another secondary lesion. This identification included a clinical and histologic differential diagnosis of metastatic cervical cancer versus a new primary cancer or metastatic cancer from another site. We used a routine multiplex real-time PCR (rt-PCR) Anyplex(TM) II HPV28 (Seegene, Seoul, Republic of Korea) to detect the high-risk (HR)-HPV genome in the distant lesions in these patients. Results: Eight cases of cervical cancer with a new secondary lesion were identified. In seven, HR-HPV DNA was detected in the biopsy of the distant lesion, which confirmed the diagnosis of cervical cancer metastasis. In the remaining case, no HPV was detected in the secondary lung biopsy, confirming the diagnosis of new primary lung cancer. Conclusion: Our results pave the way for HPV molecular genotyping use in cases of newly diagnosed distant lesions in patients with a history of HPV cervical neoplasia by using a routine diagnosis process to complete the clinical and histologic differential diagnosis when confronted with ambiguous situations. |
format | Online Article Text |
id | pubmed-9961249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99612492023-02-26 HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis Montero-Macías, Rosa Coronado, Pluvio J. Robillard, Nicolas Veyer, David Villefranque, Vincent Le Frére-Belda, Marie-Aude Auberger, Elisabeth Bitolog, Pauline Stankovic, Ivana Bélec, Laurent Bats, Anne-Sophie Lécuru, Fabrice Péré, Hélène J Pers Med Communication Background: Differentiating metastatic cervical cancer from another primary tumor can be difficult in patients with a history of cervical cancer and a distant lesion. The use of routine HPV molecular detection and genotyping tests could help in these cases. The objective of this study was to identify if an easy-to-use HPV molecular genotyping assay would allow differentiating between HPV tumor metastasis and a new independent primary non-HPV-induced tumor. Materials and Methods: Between 2010 and 2020, we identified patients with a primary cervical carcinoma who also had another secondary lesion. This identification included a clinical and histologic differential diagnosis of metastatic cervical cancer versus a new primary cancer or metastatic cancer from another site. We used a routine multiplex real-time PCR (rt-PCR) Anyplex(TM) II HPV28 (Seegene, Seoul, Republic of Korea) to detect the high-risk (HR)-HPV genome in the distant lesions in these patients. Results: Eight cases of cervical cancer with a new secondary lesion were identified. In seven, HR-HPV DNA was detected in the biopsy of the distant lesion, which confirmed the diagnosis of cervical cancer metastasis. In the remaining case, no HPV was detected in the secondary lung biopsy, confirming the diagnosis of new primary lung cancer. Conclusion: Our results pave the way for HPV molecular genotyping use in cases of newly diagnosed distant lesions in patients with a history of HPV cervical neoplasia by using a routine diagnosis process to complete the clinical and histologic differential diagnosis when confronted with ambiguous situations. MDPI 2023-01-19 /pmc/articles/PMC9961249/ /pubmed/36836411 http://dx.doi.org/10.3390/jpm13020177 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Montero-Macías, Rosa Coronado, Pluvio J. Robillard, Nicolas Veyer, David Villefranque, Vincent Le Frére-Belda, Marie-Aude Auberger, Elisabeth Bitolog, Pauline Stankovic, Ivana Bélec, Laurent Bats, Anne-Sophie Lécuru, Fabrice Péré, Hélène HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title | HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title_full | HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title_fullStr | HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title_full_unstemmed | HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title_short | HPV Molecular Genotyping as a Differential Diagnosis Tool in Cervical Cancer Metastasis |
title_sort | hpv molecular genotyping as a differential diagnosis tool in cervical cancer metastasis |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961249/ https://www.ncbi.nlm.nih.gov/pubmed/36836411 http://dx.doi.org/10.3390/jpm13020177 |
work_keys_str_mv | AT monteromaciasrosa hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT coronadopluvioj hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT robillardnicolas hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT veyerdavid hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT villefranquevincent hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT lefrerebeldamarieaude hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT aubergerelisabeth hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT bitologpauline hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT stankovicivana hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT beleclaurent hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT batsannesophie hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT lecurufabrice hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis AT perehelene hpvmoleculargenotypingasadifferentialdiagnosistoolincervicalcancermetastasis |