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Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis

The reduced form of nicotinamide adenine dinucleotide (NADH) is crucial in cellular metabolism. During hypoxia, NADH accumulation results from anaerobic cytoplasmic glycolysis and impaired mitochondrial function. This study aimed to compare the dynamic changes in the 460-nm forearm skin fluorescence...

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Autores principales: Pawlak-Chomicka, Regina, Chomicki, Wojciech, Krauze, Tomasz, Uruski, Paweł, Guzik, Maria, Piskorski, Jarosław, Tykarski, Andrzej, Guzik, Przemysław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961528/
https://www.ncbi.nlm.nih.gov/pubmed/36835783
http://dx.doi.org/10.3390/jcm12041247
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author Pawlak-Chomicka, Regina
Chomicki, Wojciech
Krauze, Tomasz
Uruski, Paweł
Guzik, Maria
Piskorski, Jarosław
Tykarski, Andrzej
Guzik, Przemysław
author_facet Pawlak-Chomicka, Regina
Chomicki, Wojciech
Krauze, Tomasz
Uruski, Paweł
Guzik, Maria
Piskorski, Jarosław
Tykarski, Andrzej
Guzik, Przemysław
author_sort Pawlak-Chomicka, Regina
collection PubMed
description The reduced form of nicotinamide adenine dinucleotide (NADH) is crucial in cellular metabolism. During hypoxia, NADH accumulation results from anaerobic cytoplasmic glycolysis and impaired mitochondrial function. This study aimed to compare the dynamic changes in the 460-nm forearm skin fluorescence, which reflects cellular NADH content, during transient ischaemia between healthy individuals and patients with newly diagnosed, untreated essential hypertension (HA). Sixteen healthy volunteers and sixty-five patients with HA underwent non-invasive measurement of forearm skin NADH content using the Flow Mediated Skin Fluorescence (FMSF) method at rest and during a 100-s transient ischaemia induced by inflation of the brachial cuff. The fluorescent signal was sampled at 25 Hz. All samples were normalised to the end of the ischaemic phase, which is the most stable phase of the whole recording. Slope values of 1 s linear regressions were determined for every 25-sample neighbouring set. The 1-s slopes in the early phase of skin ischaemia, indicating quicker hypoxia-induced NADH accumulation in skin, were significantly higher in patients with HA than in healthy individuals. These findings suggest that some protecting mechanisms postponing the early consequences of early cellular hypoxia and premature NADH accumulation during skin ischaemia are impaired in patients with untreated HA. Further studies are needed to investigate this phenomenon.
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spelling pubmed-99615282023-02-26 Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis Pawlak-Chomicka, Regina Chomicki, Wojciech Krauze, Tomasz Uruski, Paweł Guzik, Maria Piskorski, Jarosław Tykarski, Andrzej Guzik, Przemysław J Clin Med Article The reduced form of nicotinamide adenine dinucleotide (NADH) is crucial in cellular metabolism. During hypoxia, NADH accumulation results from anaerobic cytoplasmic glycolysis and impaired mitochondrial function. This study aimed to compare the dynamic changes in the 460-nm forearm skin fluorescence, which reflects cellular NADH content, during transient ischaemia between healthy individuals and patients with newly diagnosed, untreated essential hypertension (HA). Sixteen healthy volunteers and sixty-five patients with HA underwent non-invasive measurement of forearm skin NADH content using the Flow Mediated Skin Fluorescence (FMSF) method at rest and during a 100-s transient ischaemia induced by inflation of the brachial cuff. The fluorescent signal was sampled at 25 Hz. All samples were normalised to the end of the ischaemic phase, which is the most stable phase of the whole recording. Slope values of 1 s linear regressions were determined for every 25-sample neighbouring set. The 1-s slopes in the early phase of skin ischaemia, indicating quicker hypoxia-induced NADH accumulation in skin, were significantly higher in patients with HA than in healthy individuals. These findings suggest that some protecting mechanisms postponing the early consequences of early cellular hypoxia and premature NADH accumulation during skin ischaemia are impaired in patients with untreated HA. Further studies are needed to investigate this phenomenon. MDPI 2023-02-04 /pmc/articles/PMC9961528/ /pubmed/36835783 http://dx.doi.org/10.3390/jcm12041247 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pawlak-Chomicka, Regina
Chomicki, Wojciech
Krauze, Tomasz
Uruski, Paweł
Guzik, Maria
Piskorski, Jarosław
Tykarski, Andrzej
Guzik, Przemysław
Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title_full Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title_fullStr Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title_full_unstemmed Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title_short Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis
title_sort investigating the ischaemic phase of skin nadh fluorescence dynamics in recently diagnosed primary hypertension: a time series analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961528/
https://www.ncbi.nlm.nih.gov/pubmed/36835783
http://dx.doi.org/10.3390/jcm12041247
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