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Coconut Callus Initiation for Cell Suspension Culture
The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961714/ https://www.ncbi.nlm.nih.gov/pubmed/36840315 http://dx.doi.org/10.3390/plants12040968 |
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author | Kong, Eveline Y. Y. Biddle, Julianne Kalaipandian, Sundaravelpandian Adkins, Steve W. |
author_facet | Kong, Eveline Y. Y. Biddle, Julianne Kalaipandian, Sundaravelpandian Adkins, Steve W. |
author_sort | Kong, Eveline Y. Y. |
collection | PubMed |
description | The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species. |
format | Online Article Text |
id | pubmed-9961714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99617142023-02-26 Coconut Callus Initiation for Cell Suspension Culture Kong, Eveline Y. Y. Biddle, Julianne Kalaipandian, Sundaravelpandian Adkins, Steve W. Plants (Basel) Article The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species. MDPI 2023-02-20 /pmc/articles/PMC9961714/ /pubmed/36840315 http://dx.doi.org/10.3390/plants12040968 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kong, Eveline Y. Y. Biddle, Julianne Kalaipandian, Sundaravelpandian Adkins, Steve W. Coconut Callus Initiation for Cell Suspension Culture |
title | Coconut Callus Initiation for Cell Suspension Culture |
title_full | Coconut Callus Initiation for Cell Suspension Culture |
title_fullStr | Coconut Callus Initiation for Cell Suspension Culture |
title_full_unstemmed | Coconut Callus Initiation for Cell Suspension Culture |
title_short | Coconut Callus Initiation for Cell Suspension Culture |
title_sort | coconut callus initiation for cell suspension culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961714/ https://www.ncbi.nlm.nih.gov/pubmed/36840315 http://dx.doi.org/10.3390/plants12040968 |
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