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Coconut Callus Initiation for Cell Suspension Culture

The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for...

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Autores principales: Kong, Eveline Y. Y., Biddle, Julianne, Kalaipandian, Sundaravelpandian, Adkins, Steve W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961714/
https://www.ncbi.nlm.nih.gov/pubmed/36840315
http://dx.doi.org/10.3390/plants12040968
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author Kong, Eveline Y. Y.
Biddle, Julianne
Kalaipandian, Sundaravelpandian
Adkins, Steve W.
author_facet Kong, Eveline Y. Y.
Biddle, Julianne
Kalaipandian, Sundaravelpandian
Adkins, Steve W.
author_sort Kong, Eveline Y. Y.
collection PubMed
description The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species.
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spelling pubmed-99617142023-02-26 Coconut Callus Initiation for Cell Suspension Culture Kong, Eveline Y. Y. Biddle, Julianne Kalaipandian, Sundaravelpandian Adkins, Steve W. Plants (Basel) Article The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species. MDPI 2023-02-20 /pmc/articles/PMC9961714/ /pubmed/36840315 http://dx.doi.org/10.3390/plants12040968 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kong, Eveline Y. Y.
Biddle, Julianne
Kalaipandian, Sundaravelpandian
Adkins, Steve W.
Coconut Callus Initiation for Cell Suspension Culture
title Coconut Callus Initiation for Cell Suspension Culture
title_full Coconut Callus Initiation for Cell Suspension Culture
title_fullStr Coconut Callus Initiation for Cell Suspension Culture
title_full_unstemmed Coconut Callus Initiation for Cell Suspension Culture
title_short Coconut Callus Initiation for Cell Suspension Culture
title_sort coconut callus initiation for cell suspension culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961714/
https://www.ncbi.nlm.nih.gov/pubmed/36840315
http://dx.doi.org/10.3390/plants12040968
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