Cargando…

Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters

Glycidyl fatty acid esters (GEs) can be found in food, and they can be converted into genotoxic animal carcinogen glycidol in vivo by the action of lipase. This study examined whether human ingestion of charbroiled pork containing high levels of GEs (300 µg/day) increased glycidol–hemoglobin adduct...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimamura, Yuko, Inagaki, Ryo, Oike, Minami, Wada, Yuri, Honda, Hiroshi, Masuda, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961728/
https://www.ncbi.nlm.nih.gov/pubmed/36851049
http://dx.doi.org/10.3390/toxics11020175
_version_ 1784895826230771712
author Shimamura, Yuko
Inagaki, Ryo
Oike, Minami
Wada, Yuri
Honda, Hiroshi
Masuda, Shuichi
author_facet Shimamura, Yuko
Inagaki, Ryo
Oike, Minami
Wada, Yuri
Honda, Hiroshi
Masuda, Shuichi
author_sort Shimamura, Yuko
collection PubMed
description Glycidyl fatty acid esters (GEs) can be found in food, and they can be converted into genotoxic animal carcinogen glycidol in vivo by the action of lipase. This study examined whether human ingestion of charbroiled pork containing high levels of GEs (300 µg/day) increased glycidol–hemoglobin adduct (diHOPrVal), a marker of internal exposure to glycidol using LC-MS/MS. Contrary to expectation, the diHOPrVal value before ingesting charbroiled pork was 3.11 ± 1.10 pmol/g globin, which slightly decreased to 2.48 ± 0.47 pmol/g globin after 5 days of consumption. The decrease in lipase activity caused by the continuous consumption of lipid-rich foods such as meat in humans might decrease internal exposure to glycidol released from its esters. Thus, lipase activity was measured in C57/BL6J mice fed a high-fat diet (HFD) for 8 weeks, and diHOPrVal formation was measured after the administration of glycidyl oleate. Lipase activity was significantly lower in the HFD group than in the normal diet group. The amount of diHOPrVal was reduced in the HFD group. Therefore, the lipase activity was reduced by HFD, thereby decreasing the degradation of glycidol from glycidyl oleate. These results indicate that changes in lipase activity depending on the amount of lipids in the diet may affect the assessment of GEs exposure, and monitoring the lipase activity would provide a comprehensive understanding of exposure assessment.
format Online
Article
Text
id pubmed-9961728
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99617282023-02-26 Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters Shimamura, Yuko Inagaki, Ryo Oike, Minami Wada, Yuri Honda, Hiroshi Masuda, Shuichi Toxics Article Glycidyl fatty acid esters (GEs) can be found in food, and they can be converted into genotoxic animal carcinogen glycidol in vivo by the action of lipase. This study examined whether human ingestion of charbroiled pork containing high levels of GEs (300 µg/day) increased glycidol–hemoglobin adduct (diHOPrVal), a marker of internal exposure to glycidol using LC-MS/MS. Contrary to expectation, the diHOPrVal value before ingesting charbroiled pork was 3.11 ± 1.10 pmol/g globin, which slightly decreased to 2.48 ± 0.47 pmol/g globin after 5 days of consumption. The decrease in lipase activity caused by the continuous consumption of lipid-rich foods such as meat in humans might decrease internal exposure to glycidol released from its esters. Thus, lipase activity was measured in C57/BL6J mice fed a high-fat diet (HFD) for 8 weeks, and diHOPrVal formation was measured after the administration of glycidyl oleate. Lipase activity was significantly lower in the HFD group than in the normal diet group. The amount of diHOPrVal was reduced in the HFD group. Therefore, the lipase activity was reduced by HFD, thereby decreasing the degradation of glycidol from glycidyl oleate. These results indicate that changes in lipase activity depending on the amount of lipids in the diet may affect the assessment of GEs exposure, and monitoring the lipase activity would provide a comprehensive understanding of exposure assessment. MDPI 2023-02-14 /pmc/articles/PMC9961728/ /pubmed/36851049 http://dx.doi.org/10.3390/toxics11020175 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimamura, Yuko
Inagaki, Ryo
Oike, Minami
Wada, Yuri
Honda, Hiroshi
Masuda, Shuichi
Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title_full Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title_fullStr Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title_full_unstemmed Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title_short Potential Role of Lipase Activity on the Internal Exposure Assessment of Glycidol Released from Its Fatty Acid Esters
title_sort potential role of lipase activity on the internal exposure assessment of glycidol released from its fatty acid esters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961728/
https://www.ncbi.nlm.nih.gov/pubmed/36851049
http://dx.doi.org/10.3390/toxics11020175
work_keys_str_mv AT shimamurayuko potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters
AT inagakiryo potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters
AT oikeminami potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters
AT wadayuri potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters
AT hondahiroshi potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters
AT masudashuichi potentialroleoflipaseactivityontheinternalexposureassessmentofglycidolreleasedfromitsfattyacidesters