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Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation

Herein, we evaluated the hepatic lipid metabolic profiles of bovine fetuses in response to maternal vitamin and mineral supplementation (VMSUP; supplemented (VTM) or not (NoVTM)) and two different rates of gain (GAIN; low gain (LG), 0.28 kg/d, or moderate gain (MG), 0.79 kg/d). Crossbred Angus heife...

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Autores principales: Menezes, Ana Clara B., Dahlen, Carl R., McCarthy, Kacie L., Kassetas, Cierrah J., Baumgaertner, Friederike, Kirsch, James D., Dorsam, Sheri T., Neville, Tammi L., Ward, Alison K., Borowicz, Pawel P., Reynolds, Lawrence P., Sedivec, Kevin K., Forcherio, J. Chris, Scott, Ronald, Caton, Joel S., Crouse, Matthew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961797/
https://www.ncbi.nlm.nih.gov/pubmed/36837794
http://dx.doi.org/10.3390/metabo13020175
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author Menezes, Ana Clara B.
Dahlen, Carl R.
McCarthy, Kacie L.
Kassetas, Cierrah J.
Baumgaertner, Friederike
Kirsch, James D.
Dorsam, Sheri T.
Neville, Tammi L.
Ward, Alison K.
Borowicz, Pawel P.
Reynolds, Lawrence P.
Sedivec, Kevin K.
Forcherio, J. Chris
Scott, Ronald
Caton, Joel S.
Crouse, Matthew S.
author_facet Menezes, Ana Clara B.
Dahlen, Carl R.
McCarthy, Kacie L.
Kassetas, Cierrah J.
Baumgaertner, Friederike
Kirsch, James D.
Dorsam, Sheri T.
Neville, Tammi L.
Ward, Alison K.
Borowicz, Pawel P.
Reynolds, Lawrence P.
Sedivec, Kevin K.
Forcherio, J. Chris
Scott, Ronald
Caton, Joel S.
Crouse, Matthew S.
author_sort Menezes, Ana Clara B.
collection PubMed
description Herein, we evaluated the hepatic lipid metabolic profiles of bovine fetuses in response to maternal vitamin and mineral supplementation (VMSUP; supplemented (VTM) or not (NoVTM)) and two different rates of gain (GAIN; low gain (LG), 0.28 kg/d, or moderate gain (MG), 0.79 kg/d). Crossbred Angus heifers (n = 35; initial BW = 359.5 ± 7.1 kg) were randomly assigned to a 2 × 2 factorial arrangement, resulting in the following treatment combinations: NoVTM-LG (n = 9), NoVTM-MG (n = 9), VTM-LG (n = 9), and VTM-MG (n = 8). Heifers received their treatments until d 83 of gestation, when they were ovariohysterectomized. Fetuses were harvested and liver samples were analyzed via ultrahigh-performance liquid chromatography–tandem mass spectroscopy to characterize lipid profiles and abundances. We identified 374 biochemicals/metabolites belonging to 57 sub-pathways of the lipid metabolism super-pathway. The majority of the biochemicals/metabolites (n = 152) were significantly affected by the main effect of GAIN. Maternal moderate rates of gain resulted in greater abundances (p ≤ 0.0001) of ω-3 fatty acids (eicosapentaenoate, docosapentaenoate, and docosahexaenoate) and lower abundances (p ≤ 0.0001) of ω-6 fatty acids. Further, MG resulted in the accumulation of several diacylglycerols and depletion of the majority of the monoacylglycerols. Concentrations of nearly all acylcarnitines (p ≤ 0.03) were decreased in VTM-LG fetal livers compared to all other treatment combinations, indicating a greater rate of complete oxidation of fatty acids. Levels of secondary bile acids were impacted by VMSUP, being greater (p ≤ 0.0048) in NoVTM than in VTM fetal livers. Moreover, NoVTM combined with lower rate of gain resulted in greater concentrations of most secondary bile acid biochemicals/metabolites. These data indicate that maternal diet influenced and altered fetal hepatic lipid composition in the first trimester of gestation. Maternal body weight gain exerted a greater influence on fetal lipid profiles than vitamin and mineral supplementation. Specifically, lower rate of gain (0.28 kg/d) resulted in an increased abundance of the majority of the biochemicals/metabolites identified in this study.
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spelling pubmed-99617972023-02-26 Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation Menezes, Ana Clara B. Dahlen, Carl R. McCarthy, Kacie L. Kassetas, Cierrah J. Baumgaertner, Friederike Kirsch, James D. Dorsam, Sheri T. Neville, Tammi L. Ward, Alison K. Borowicz, Pawel P. Reynolds, Lawrence P. Sedivec, Kevin K. Forcherio, J. Chris Scott, Ronald Caton, Joel S. Crouse, Matthew S. Metabolites Article Herein, we evaluated the hepatic lipid metabolic profiles of bovine fetuses in response to maternal vitamin and mineral supplementation (VMSUP; supplemented (VTM) or not (NoVTM)) and two different rates of gain (GAIN; low gain (LG), 0.28 kg/d, or moderate gain (MG), 0.79 kg/d). Crossbred Angus heifers (n = 35; initial BW = 359.5 ± 7.1 kg) were randomly assigned to a 2 × 2 factorial arrangement, resulting in the following treatment combinations: NoVTM-LG (n = 9), NoVTM-MG (n = 9), VTM-LG (n = 9), and VTM-MG (n = 8). Heifers received their treatments until d 83 of gestation, when they were ovariohysterectomized. Fetuses were harvested and liver samples were analyzed via ultrahigh-performance liquid chromatography–tandem mass spectroscopy to characterize lipid profiles and abundances. We identified 374 biochemicals/metabolites belonging to 57 sub-pathways of the lipid metabolism super-pathway. The majority of the biochemicals/metabolites (n = 152) were significantly affected by the main effect of GAIN. Maternal moderate rates of gain resulted in greater abundances (p ≤ 0.0001) of ω-3 fatty acids (eicosapentaenoate, docosapentaenoate, and docosahexaenoate) and lower abundances (p ≤ 0.0001) of ω-6 fatty acids. Further, MG resulted in the accumulation of several diacylglycerols and depletion of the majority of the monoacylglycerols. Concentrations of nearly all acylcarnitines (p ≤ 0.03) were decreased in VTM-LG fetal livers compared to all other treatment combinations, indicating a greater rate of complete oxidation of fatty acids. Levels of secondary bile acids were impacted by VMSUP, being greater (p ≤ 0.0048) in NoVTM than in VTM fetal livers. Moreover, NoVTM combined with lower rate of gain resulted in greater concentrations of most secondary bile acid biochemicals/metabolites. These data indicate that maternal diet influenced and altered fetal hepatic lipid composition in the first trimester of gestation. Maternal body weight gain exerted a greater influence on fetal lipid profiles than vitamin and mineral supplementation. Specifically, lower rate of gain (0.28 kg/d) resulted in an increased abundance of the majority of the biochemicals/metabolites identified in this study. MDPI 2023-01-25 /pmc/articles/PMC9961797/ /pubmed/36837794 http://dx.doi.org/10.3390/metabo13020175 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Menezes, Ana Clara B.
Dahlen, Carl R.
McCarthy, Kacie L.
Kassetas, Cierrah J.
Baumgaertner, Friederike
Kirsch, James D.
Dorsam, Sheri T.
Neville, Tammi L.
Ward, Alison K.
Borowicz, Pawel P.
Reynolds, Lawrence P.
Sedivec, Kevin K.
Forcherio, J. Chris
Scott, Ronald
Caton, Joel S.
Crouse, Matthew S.
Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title_full Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title_fullStr Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title_full_unstemmed Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title_short Fetal Hepatic Lipidome Is More Greatly Affected by Maternal Rate of Gain Compared with Vitamin and Mineral Supplementation at day 83 of Gestation
title_sort fetal hepatic lipidome is more greatly affected by maternal rate of gain compared with vitamin and mineral supplementation at day 83 of gestation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961797/
https://www.ncbi.nlm.nih.gov/pubmed/36837794
http://dx.doi.org/10.3390/metabo13020175
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