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Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration

As a member of TALE family, Meis1 has been proven to regulate cell proliferation and differentiation during cell fate commitment; however, the mechanism is still not fully understood. The planarian, which has an abundance of stem cells (neoblasts) responsible for regenerating any organ after injury,...

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Autores principales: Wang, Shaocong, Sun, Yujia, Liu, Xiaomai, Guo, Yajun, Huang, Yongding, Zhang, Shoutao, Tian, Qingnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961902/
https://www.ncbi.nlm.nih.gov/pubmed/36834910
http://dx.doi.org/10.3390/ijms24043505
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author Wang, Shaocong
Sun, Yujia
Liu, Xiaomai
Guo, Yajun
Huang, Yongding
Zhang, Shoutao
Tian, Qingnan
author_facet Wang, Shaocong
Sun, Yujia
Liu, Xiaomai
Guo, Yajun
Huang, Yongding
Zhang, Shoutao
Tian, Qingnan
author_sort Wang, Shaocong
collection PubMed
description As a member of TALE family, Meis1 has been proven to regulate cell proliferation and differentiation during cell fate commitment; however, the mechanism is still not fully understood. The planarian, which has an abundance of stem cells (neoblasts) responsible for regenerating any organ after injury, is an ideal model for studying the mechanisms of tissue identity determination. Here, we characterized a planarian homolog of Meis1 from the planarian Dugesia japonica. Importantly, we found that knockdown of DjMeis1 inhibits the differentiation of neoblasts into eye progenitor cells and results in an eyeless phenotype with normal central nervous system. Furthermore, we observed that DjMeis1 is required for the activation of Wnt signaling pathway by promoting the Djwnt1 expression during posterior regeneration. The silencing of DjMeis1 suppresses the expression of Djwnt1 and results in the inability to reconstruct posterior poles. In general, our findings indicated that DjMeis1 acts as a trigger for the activation of eye and tail regeneration by regulating the differentiation of eye progenitor cells and the formation of posterior poles, respectively.
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spelling pubmed-99619022023-02-26 Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration Wang, Shaocong Sun, Yujia Liu, Xiaomai Guo, Yajun Huang, Yongding Zhang, Shoutao Tian, Qingnan Int J Mol Sci Article As a member of TALE family, Meis1 has been proven to regulate cell proliferation and differentiation during cell fate commitment; however, the mechanism is still not fully understood. The planarian, which has an abundance of stem cells (neoblasts) responsible for regenerating any organ after injury, is an ideal model for studying the mechanisms of tissue identity determination. Here, we characterized a planarian homolog of Meis1 from the planarian Dugesia japonica. Importantly, we found that knockdown of DjMeis1 inhibits the differentiation of neoblasts into eye progenitor cells and results in an eyeless phenotype with normal central nervous system. Furthermore, we observed that DjMeis1 is required for the activation of Wnt signaling pathway by promoting the Djwnt1 expression during posterior regeneration. The silencing of DjMeis1 suppresses the expression of Djwnt1 and results in the inability to reconstruct posterior poles. In general, our findings indicated that DjMeis1 acts as a trigger for the activation of eye and tail regeneration by regulating the differentiation of eye progenitor cells and the formation of posterior poles, respectively. MDPI 2023-02-09 /pmc/articles/PMC9961902/ /pubmed/36834910 http://dx.doi.org/10.3390/ijms24043505 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Shaocong
Sun, Yujia
Liu, Xiaomai
Guo, Yajun
Huang, Yongding
Zhang, Shoutao
Tian, Qingnan
Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title_full Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title_fullStr Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title_full_unstemmed Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title_short Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration
title_sort meis1 controls the differentiation of eye progenitor cells and the formation of posterior poles during planarian regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961902/
https://www.ncbi.nlm.nih.gov/pubmed/36834910
http://dx.doi.org/10.3390/ijms24043505
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