Cargando…

Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’

Black rot (BR), caused by Guignardia bidwellii, is an emergent fungal disease threatening viticulture and affecting several mildew-tolerant varieties. However, its genetic bases are not fully dissected yet. For this purpose, a segregating population derived from the cross ‘Merzling’ (hybrid, resista...

Descripción completa

Detalles Bibliográficos
Autores principales: Bettinelli, Paola, Nicolini, Daniela, Costantini, Laura, Stefanini, Marco, Hausmann, Ludger, Vezzulli, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961920/
https://www.ncbi.nlm.nih.gov/pubmed/36834979
http://dx.doi.org/10.3390/ijms24043568
_version_ 1784895874806054912
author Bettinelli, Paola
Nicolini, Daniela
Costantini, Laura
Stefanini, Marco
Hausmann, Ludger
Vezzulli, Silvia
author_facet Bettinelli, Paola
Nicolini, Daniela
Costantini, Laura
Stefanini, Marco
Hausmann, Ludger
Vezzulli, Silvia
author_sort Bettinelli, Paola
collection PubMed
description Black rot (BR), caused by Guignardia bidwellii, is an emergent fungal disease threatening viticulture and affecting several mildew-tolerant varieties. However, its genetic bases are not fully dissected yet. For this purpose, a segregating population derived from the cross ‘Merzling’ (hybrid, resistant) × ‘Teroldego’ (V. vinifera, susceptible) was evaluated for BR resistance at the shoot and bunch level. The progeny was genotyped with the GrapeReSeq Illumina 20K SNPchip, and 7175 SNPs were combined with 194 SSRs to generate a high-density linkage map of 1677 cM. The QTL analysis based on shoot trials confirmed the previously identified Resistance to Guignardia bidwellii (Rgb)1 locus on chromosome 14, which explained up to 29.2% of the phenotypic variance, reducing the genomic interval from 2.4 to 0.7 Mb. Upstream of Rgb1, this study revealed a new QTL explaining up to 79.9% of the variance for bunch resistance, designated Rgb3. The physical region encompassing the two QTLs does not underlie annotated resistance (R)-genes. The Rgb1 locus resulted enriched in genes belonging to phloem dynamics and mitochondrial proton transfer, while Rgb3 presented a cluster of pathogenesis-related Germin-like protein genes, promoters of the programmed cell death. These outcomes suggest a strong involvement of mitochondrial oxidative burst and phloem occlusion in BR resistance mechanisms and provide new molecular tools for grapevine marker-assisted breeding.
format Online
Article
Text
id pubmed-9961920
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99619202023-02-26 Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’ Bettinelli, Paola Nicolini, Daniela Costantini, Laura Stefanini, Marco Hausmann, Ludger Vezzulli, Silvia Int J Mol Sci Article Black rot (BR), caused by Guignardia bidwellii, is an emergent fungal disease threatening viticulture and affecting several mildew-tolerant varieties. However, its genetic bases are not fully dissected yet. For this purpose, a segregating population derived from the cross ‘Merzling’ (hybrid, resistant) × ‘Teroldego’ (V. vinifera, susceptible) was evaluated for BR resistance at the shoot and bunch level. The progeny was genotyped with the GrapeReSeq Illumina 20K SNPchip, and 7175 SNPs were combined with 194 SSRs to generate a high-density linkage map of 1677 cM. The QTL analysis based on shoot trials confirmed the previously identified Resistance to Guignardia bidwellii (Rgb)1 locus on chromosome 14, which explained up to 29.2% of the phenotypic variance, reducing the genomic interval from 2.4 to 0.7 Mb. Upstream of Rgb1, this study revealed a new QTL explaining up to 79.9% of the variance for bunch resistance, designated Rgb3. The physical region encompassing the two QTLs does not underlie annotated resistance (R)-genes. The Rgb1 locus resulted enriched in genes belonging to phloem dynamics and mitochondrial proton transfer, while Rgb3 presented a cluster of pathogenesis-related Germin-like protein genes, promoters of the programmed cell death. These outcomes suggest a strong involvement of mitochondrial oxidative burst and phloem occlusion in BR resistance mechanisms and provide new molecular tools for grapevine marker-assisted breeding. MDPI 2023-02-10 /pmc/articles/PMC9961920/ /pubmed/36834979 http://dx.doi.org/10.3390/ijms24043568 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bettinelli, Paola
Nicolini, Daniela
Costantini, Laura
Stefanini, Marco
Hausmann, Ludger
Vezzulli, Silvia
Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title_full Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title_fullStr Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title_full_unstemmed Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title_short Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’
title_sort towards marker-assisted breeding for black rot bunch resistance: identification of a major qtl in the grapevine cultivar ‘merzling’
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961920/
https://www.ncbi.nlm.nih.gov/pubmed/36834979
http://dx.doi.org/10.3390/ijms24043568
work_keys_str_mv AT bettinellipaola towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling
AT nicolinidaniela towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling
AT costantinilaura towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling
AT stefaninimarco towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling
AT hausmannludger towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling
AT vezzullisilvia towardsmarkerassistedbreedingforblackrotbunchresistanceidentificationofamajorqtlinthegrapevinecultivarmerzling