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Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration
Triamcinolone acetonide (TA) is a powerful anti-inflammatory drug used in the treatment of inflammatory ocular disorders; however, its poor aqueous solubility and ocular anatomical barriers hinder optimal treatment. The aim of this work was to obtain triamcinolone acetonide nanocrystals (TA-NC) to i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962019/ https://www.ncbi.nlm.nih.gov/pubmed/36840006 http://dx.doi.org/10.3390/pharmaceutics15020683 |
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author | Formica, María Lina Awde Alfonso, Hamoudi Ghassan Paredes, Alejandro Javier Melian, María Elisa Camacho, Nahuel Matías Faccio, Ricardo Tártara, Luis Ignacio Palma, Santiago Daniel |
author_facet | Formica, María Lina Awde Alfonso, Hamoudi Ghassan Paredes, Alejandro Javier Melian, María Elisa Camacho, Nahuel Matías Faccio, Ricardo Tártara, Luis Ignacio Palma, Santiago Daniel |
author_sort | Formica, María Lina |
collection | PubMed |
description | Triamcinolone acetonide (TA) is a powerful anti-inflammatory drug used in the treatment of inflammatory ocular disorders; however, its poor aqueous solubility and ocular anatomical barriers hinder optimal treatment. The aim of this work was to obtain triamcinolone acetonide nanocrystals (TA-NC) to improve ocular corticosteroid therapy. Self-dispersible TA-NC were prepared by the bead milling technique followed by spray-drying, exhaustively characterized and then evaluated in vivo in an ocular model of endotoxin-induced uveitis (EIU). Self-dispersible TA-NC presented an average particle size of 257 ± 30 nm, a narrow size distribution and a zeta potential of −25 ± 3 mV, which remained unchanged for 120 days under storage conditions at 25 °C. In addition, SEM studies of the TA-NC showed uniform and spherical morphology, and FTIR and XRDP analyses indicated no apparent chemical and crystallinity changes. The subconjunctival administration of TA-NC in albino male white rabbits showed no clinical signs of ocular damage. In vivo studies proved that treatment with self-dispersible TA-NC alleviated the inflammatory response in the anterior chamber and iris in EUI rabbit eyes. Dispersible TA-NC are a promising approach to obtaining a novel nanometric TA formulation for ocular disorders. |
format | Online Article Text |
id | pubmed-9962019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99620192023-02-26 Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration Formica, María Lina Awde Alfonso, Hamoudi Ghassan Paredes, Alejandro Javier Melian, María Elisa Camacho, Nahuel Matías Faccio, Ricardo Tártara, Luis Ignacio Palma, Santiago Daniel Pharmaceutics Article Triamcinolone acetonide (TA) is a powerful anti-inflammatory drug used in the treatment of inflammatory ocular disorders; however, its poor aqueous solubility and ocular anatomical barriers hinder optimal treatment. The aim of this work was to obtain triamcinolone acetonide nanocrystals (TA-NC) to improve ocular corticosteroid therapy. Self-dispersible TA-NC were prepared by the bead milling technique followed by spray-drying, exhaustively characterized and then evaluated in vivo in an ocular model of endotoxin-induced uveitis (EIU). Self-dispersible TA-NC presented an average particle size of 257 ± 30 nm, a narrow size distribution and a zeta potential of −25 ± 3 mV, which remained unchanged for 120 days under storage conditions at 25 °C. In addition, SEM studies of the TA-NC showed uniform and spherical morphology, and FTIR and XRDP analyses indicated no apparent chemical and crystallinity changes. The subconjunctival administration of TA-NC in albino male white rabbits showed no clinical signs of ocular damage. In vivo studies proved that treatment with self-dispersible TA-NC alleviated the inflammatory response in the anterior chamber and iris in EUI rabbit eyes. Dispersible TA-NC are a promising approach to obtaining a novel nanometric TA formulation for ocular disorders. MDPI 2023-02-17 /pmc/articles/PMC9962019/ /pubmed/36840006 http://dx.doi.org/10.3390/pharmaceutics15020683 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Formica, María Lina Awde Alfonso, Hamoudi Ghassan Paredes, Alejandro Javier Melian, María Elisa Camacho, Nahuel Matías Faccio, Ricardo Tártara, Luis Ignacio Palma, Santiago Daniel Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title | Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title_full | Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title_fullStr | Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title_full_unstemmed | Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title_short | Development of Triamcinolone Acetonide Nanocrystals for Ocular Administration |
title_sort | development of triamcinolone acetonide nanocrystals for ocular administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962019/ https://www.ncbi.nlm.nih.gov/pubmed/36840006 http://dx.doi.org/10.3390/pharmaceutics15020683 |
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