Cargando…

Candida auris in Austria—What Is New and What Is Different

Candida auris is a novel and emerging pathogenic yeast which represents a serious global health threat. Since its first description in Japan 2009, it has been associated with large hospital outbreaks all over the world and is often resistant to more than one antifungal drug class. To date, five C. a...

Descripción completa

Detalles Bibliográficos
Autores principales: Spettel, Kathrin, Kriz, Richard, Wu, Christine, Achter, Lukas, Schmid, Stefan, Galazka, Sonia, Selitsch, Brigitte, Camp, Iris, Makristathis, Athanasios, Lagler, Heimo, Willinger, Birgit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962151/
https://www.ncbi.nlm.nih.gov/pubmed/36836244
http://dx.doi.org/10.3390/jof9020129
_version_ 1784895932528066560
author Spettel, Kathrin
Kriz, Richard
Wu, Christine
Achter, Lukas
Schmid, Stefan
Galazka, Sonia
Selitsch, Brigitte
Camp, Iris
Makristathis, Athanasios
Lagler, Heimo
Willinger, Birgit
author_facet Spettel, Kathrin
Kriz, Richard
Wu, Christine
Achter, Lukas
Schmid, Stefan
Galazka, Sonia
Selitsch, Brigitte
Camp, Iris
Makristathis, Athanasios
Lagler, Heimo
Willinger, Birgit
author_sort Spettel, Kathrin
collection PubMed
description Candida auris is a novel and emerging pathogenic yeast which represents a serious global health threat. Since its first description in Japan 2009, it has been associated with large hospital outbreaks all over the world and is often resistant to more than one antifungal drug class. To date, five C. auris isolates have been detected in Austria. Morphological characterization and antifungal susceptibility profiles against echinocandins, azoles, polyenes and pyrimidines, as well as the new antifungals ibrexafungerp and manogepix, were determined. In order to assess pathogenicity of these isolates, an infection model in Galleria mellonella was performed and whole genome sequencing (WGS) analysis was conducted to determine the phylogeographic origin. We could characterize four isolates as South Asian clade I and one isolate as African clade III. All of them had elevated minimal inhibitory concentrations to at least two different antifungal classes. The new antifungal manogepix showed high in vitro efficacy against all five C. auris isolates. One isolate, belonging to the African clade III, showed an aggregating phenotype, while the other isolates belonging to South Asian clade I were non-aggregating. In the Galleria mellonella infection model, the isolate belonging to African clade III exhibited the lowest in vivo pathogenicity. As the occurrence of C. auris increases globally, it is important to raise awareness to prevent transmission and hospital outbreaks.
format Online
Article
Text
id pubmed-9962151
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99621512023-02-26 Candida auris in Austria—What Is New and What Is Different Spettel, Kathrin Kriz, Richard Wu, Christine Achter, Lukas Schmid, Stefan Galazka, Sonia Selitsch, Brigitte Camp, Iris Makristathis, Athanasios Lagler, Heimo Willinger, Birgit J Fungi (Basel) Article Candida auris is a novel and emerging pathogenic yeast which represents a serious global health threat. Since its first description in Japan 2009, it has been associated with large hospital outbreaks all over the world and is often resistant to more than one antifungal drug class. To date, five C. auris isolates have been detected in Austria. Morphological characterization and antifungal susceptibility profiles against echinocandins, azoles, polyenes and pyrimidines, as well as the new antifungals ibrexafungerp and manogepix, were determined. In order to assess pathogenicity of these isolates, an infection model in Galleria mellonella was performed and whole genome sequencing (WGS) analysis was conducted to determine the phylogeographic origin. We could characterize four isolates as South Asian clade I and one isolate as African clade III. All of them had elevated minimal inhibitory concentrations to at least two different antifungal classes. The new antifungal manogepix showed high in vitro efficacy against all five C. auris isolates. One isolate, belonging to the African clade III, showed an aggregating phenotype, while the other isolates belonging to South Asian clade I were non-aggregating. In the Galleria mellonella infection model, the isolate belonging to African clade III exhibited the lowest in vivo pathogenicity. As the occurrence of C. auris increases globally, it is important to raise awareness to prevent transmission and hospital outbreaks. MDPI 2023-01-17 /pmc/articles/PMC9962151/ /pubmed/36836244 http://dx.doi.org/10.3390/jof9020129 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spettel, Kathrin
Kriz, Richard
Wu, Christine
Achter, Lukas
Schmid, Stefan
Galazka, Sonia
Selitsch, Brigitte
Camp, Iris
Makristathis, Athanasios
Lagler, Heimo
Willinger, Birgit
Candida auris in Austria—What Is New and What Is Different
title Candida auris in Austria—What Is New and What Is Different
title_full Candida auris in Austria—What Is New and What Is Different
title_fullStr Candida auris in Austria—What Is New and What Is Different
title_full_unstemmed Candida auris in Austria—What Is New and What Is Different
title_short Candida auris in Austria—What Is New and What Is Different
title_sort candida auris in austria—what is new and what is different
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962151/
https://www.ncbi.nlm.nih.gov/pubmed/36836244
http://dx.doi.org/10.3390/jof9020129
work_keys_str_mv AT spettelkathrin candidaaurisinaustriawhatisnewandwhatisdifferent
AT krizrichard candidaaurisinaustriawhatisnewandwhatisdifferent
AT wuchristine candidaaurisinaustriawhatisnewandwhatisdifferent
AT achterlukas candidaaurisinaustriawhatisnewandwhatisdifferent
AT schmidstefan candidaaurisinaustriawhatisnewandwhatisdifferent
AT galazkasonia candidaaurisinaustriawhatisnewandwhatisdifferent
AT selitschbrigitte candidaaurisinaustriawhatisnewandwhatisdifferent
AT campiris candidaaurisinaustriawhatisnewandwhatisdifferent
AT makristathisathanasios candidaaurisinaustriawhatisnewandwhatisdifferent
AT laglerheimo candidaaurisinaustriawhatisnewandwhatisdifferent
AT willingerbirgit candidaaurisinaustriawhatisnewandwhatisdifferent