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Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection leads to a wide range of clinical manifestations and determines the need for personalized and precision medicine. To better understand the biological determinants of this heterogeneity, we explored the plasma proteome of 43 COVID...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962231/ https://www.ncbi.nlm.nih.gov/pubmed/36834989 http://dx.doi.org/10.3390/ijms24043570 |
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author | Pagani, Lisa Chinello, Clizia Risca, Giulia Capitoli, Giulia Criscuolo, Lucrezia Lombardi, Andrea Ungaro, Riccardo Mangioni, Davide Piga, Isabella Muscatello, Antonio Blasi, Francesco Favalli, Andrea Martinovic, Martina Gori, Andrea Bandera, Alessandra Grifantini, Renata Magni, Fulvio |
author_facet | Pagani, Lisa Chinello, Clizia Risca, Giulia Capitoli, Giulia Criscuolo, Lucrezia Lombardi, Andrea Ungaro, Riccardo Mangioni, Davide Piga, Isabella Muscatello, Antonio Blasi, Francesco Favalli, Andrea Martinovic, Martina Gori, Andrea Bandera, Alessandra Grifantini, Renata Magni, Fulvio |
author_sort | Pagani, Lisa |
collection | PubMed |
description | Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection leads to a wide range of clinical manifestations and determines the need for personalized and precision medicine. To better understand the biological determinants of this heterogeneity, we explored the plasma proteome of 43 COVID-19 patients with different outcomes by an untargeted liquid chromatography-mass spectrometry approach. The comparison between asymptomatic or pauci-symptomatic subjects (MILDs), and hospitalised patients in need of oxygen support therapy (SEVEREs) highlighted 29 proteins emerged as differentially expressed: 12 overexpressed in MILDs and 17 in SEVEREs. Moreover, a supervised analysis based on a decision-tree recognised three proteins (Fetuin-A, Ig lambda-2chain-C-region, Vitronectin) that are able to robustly discriminate between the two classes independently from the infection stage. In silico functional annotation of the 29 deregulated proteins pinpointed several functions possibly related to the severity; no pathway was associated exclusively to MILDs, while several only to SEVEREs, and some associated to both MILDs and SEVEREs; SARS-CoV-2 signalling pathway was significantly enriched by proteins up-expressed in SEVEREs (SAA1/2, CRP, HP, LRG1) and in MILDs (GSN, HRG). In conclusion, our analysis could provide key information for ‘proteomically’ defining possible upstream mechanisms and mediators triggering or limiting the domino effect of the immune-related response and characterizing severe exacerbations. |
format | Online Article Text |
id | pubmed-9962231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99622312023-02-26 Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation Pagani, Lisa Chinello, Clizia Risca, Giulia Capitoli, Giulia Criscuolo, Lucrezia Lombardi, Andrea Ungaro, Riccardo Mangioni, Davide Piga, Isabella Muscatello, Antonio Blasi, Francesco Favalli, Andrea Martinovic, Martina Gori, Andrea Bandera, Alessandra Grifantini, Renata Magni, Fulvio Int J Mol Sci Article Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection leads to a wide range of clinical manifestations and determines the need for personalized and precision medicine. To better understand the biological determinants of this heterogeneity, we explored the plasma proteome of 43 COVID-19 patients with different outcomes by an untargeted liquid chromatography-mass spectrometry approach. The comparison between asymptomatic or pauci-symptomatic subjects (MILDs), and hospitalised patients in need of oxygen support therapy (SEVEREs) highlighted 29 proteins emerged as differentially expressed: 12 overexpressed in MILDs and 17 in SEVEREs. Moreover, a supervised analysis based on a decision-tree recognised three proteins (Fetuin-A, Ig lambda-2chain-C-region, Vitronectin) that are able to robustly discriminate between the two classes independently from the infection stage. In silico functional annotation of the 29 deregulated proteins pinpointed several functions possibly related to the severity; no pathway was associated exclusively to MILDs, while several only to SEVEREs, and some associated to both MILDs and SEVEREs; SARS-CoV-2 signalling pathway was significantly enriched by proteins up-expressed in SEVEREs (SAA1/2, CRP, HP, LRG1) and in MILDs (GSN, HRG). In conclusion, our analysis could provide key information for ‘proteomically’ defining possible upstream mechanisms and mediators triggering or limiting the domino effect of the immune-related response and characterizing severe exacerbations. MDPI 2023-02-10 /pmc/articles/PMC9962231/ /pubmed/36834989 http://dx.doi.org/10.3390/ijms24043570 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pagani, Lisa Chinello, Clizia Risca, Giulia Capitoli, Giulia Criscuolo, Lucrezia Lombardi, Andrea Ungaro, Riccardo Mangioni, Davide Piga, Isabella Muscatello, Antonio Blasi, Francesco Favalli, Andrea Martinovic, Martina Gori, Andrea Bandera, Alessandra Grifantini, Renata Magni, Fulvio Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title | Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title_full | Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title_fullStr | Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title_full_unstemmed | Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title_short | Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation |
title_sort | plasma proteomic variables related to covid-19 severity: an untargeted nlc-ms/ms investigation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962231/ https://www.ncbi.nlm.nih.gov/pubmed/36834989 http://dx.doi.org/10.3390/ijms24043570 |
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