Cargando…
Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds
Salmonella enterica is considered a significant threat to the global poultry industry and public health. In recent decades, antimicrobial resistance in Salmonella enterica has attracted increasing concern throughout the world. However, limited information is available on Salmonella enterica among di...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962450/ https://www.ncbi.nlm.nih.gov/pubmed/36838355 http://dx.doi.org/10.3390/microorganisms11020390 |
_version_ | 1784896008051752960 |
---|---|
author | Shi, Wenjian Tang, Wenli Li, Yafei Han, Yu Cui, Lulu Sun, Shuhong |
author_facet | Shi, Wenjian Tang, Wenli Li, Yafei Han, Yu Cui, Lulu Sun, Shuhong |
author_sort | Shi, Wenjian |
collection | PubMed |
description | Salmonella enterica is considered a significant threat to the global poultry industry and public health. In recent decades, antimicrobial resistance in Salmonella enterica has attracted increasing concern throughout the world. However, limited information is available on Salmonella enterica among different breeds of breeder chickens. Thus, this study aimed to compare the prevalence, serotype distribution, emergence of extended-spectrum beta-lactamases (ESBLs), antimicrobial resistance, and genetic resistance mechanisms in Salmonella enterica among different breeds of breeder chickens. A total of 693 samples (dead embryos, cloacal swabs, water, feed, environmental swabs, and meconium of newly hatched chicks) were selected and cultured for Salmonella from four breeder chicken farms in Shandong province, China, representing one imported and three native breeds, and the isolates were further serotyped. Of the Salmonella isolates, susceptibility to 11 antimicrobials of 5 classes, ESBL screening, and the presence of 21 antimicrobial resistance genes were determined in the present study. Overall, 94 (13.6%) isolates were recovered, which were divided into 3 serotypes (Salmonella Pullorum (n = 36), Salmonella Thompson (n = 32), and Salmonella Enteritidis (n = 26)). The results showed that the prevalence of Salmonella enterica isolates from the imported breeds was higher compared with the three domestic breeds. Eight of the ninety-four isolates were ESBL-positive strains, which were recovered from a domestic breed chicken farm. These eight ESBL-producing isolates were serotyped to Pullorum. Surprisingly, Salmonella Enteritidis (S. enteritidis) and S. pullorum were simultaneously isolated from a single dead embryo observed among one native breed. Meanwhile, among the Salmonella isolates, 53.2% (50/94) were multidrug-resistant strains, and 44.7% (42/94) of the isolates presented resistance to at least five antibiotics. Nearly all of the isolates (97.9%, 92/94) were resistant to at least one antimicrobial; one isolate of S. Thompson was resistant to seven antimicrobial agents belonging to four different classes. The carriage rate of three resistance genes (tetA, tetB, and sul1) among isolates from the imported breeds (87%, 70%, and 65.2%) was higher than that in those from domestic breeds (35.2%, 36.6, and 14.1%). To our knowledge, this is the first report of ESBLs-producing Salmonella isolated from a Chinese native breed of breeder chickens. Our results also highlight that a high prevalence of multidrug-resistant Salmonella enterica contamination is widespread among different breeds of breeder chickens, which is a major risk of food-borne diseases and public health. |
format | Online Article Text |
id | pubmed-9962450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99624502023-02-26 Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds Shi, Wenjian Tang, Wenli Li, Yafei Han, Yu Cui, Lulu Sun, Shuhong Microorganisms Article Salmonella enterica is considered a significant threat to the global poultry industry and public health. In recent decades, antimicrobial resistance in Salmonella enterica has attracted increasing concern throughout the world. However, limited information is available on Salmonella enterica among different breeds of breeder chickens. Thus, this study aimed to compare the prevalence, serotype distribution, emergence of extended-spectrum beta-lactamases (ESBLs), antimicrobial resistance, and genetic resistance mechanisms in Salmonella enterica among different breeds of breeder chickens. A total of 693 samples (dead embryos, cloacal swabs, water, feed, environmental swabs, and meconium of newly hatched chicks) were selected and cultured for Salmonella from four breeder chicken farms in Shandong province, China, representing one imported and three native breeds, and the isolates were further serotyped. Of the Salmonella isolates, susceptibility to 11 antimicrobials of 5 classes, ESBL screening, and the presence of 21 antimicrobial resistance genes were determined in the present study. Overall, 94 (13.6%) isolates were recovered, which were divided into 3 serotypes (Salmonella Pullorum (n = 36), Salmonella Thompson (n = 32), and Salmonella Enteritidis (n = 26)). The results showed that the prevalence of Salmonella enterica isolates from the imported breeds was higher compared with the three domestic breeds. Eight of the ninety-four isolates were ESBL-positive strains, which were recovered from a domestic breed chicken farm. These eight ESBL-producing isolates were serotyped to Pullorum. Surprisingly, Salmonella Enteritidis (S. enteritidis) and S. pullorum were simultaneously isolated from a single dead embryo observed among one native breed. Meanwhile, among the Salmonella isolates, 53.2% (50/94) were multidrug-resistant strains, and 44.7% (42/94) of the isolates presented resistance to at least five antibiotics. Nearly all of the isolates (97.9%, 92/94) were resistant to at least one antimicrobial; one isolate of S. Thompson was resistant to seven antimicrobial agents belonging to four different classes. The carriage rate of three resistance genes (tetA, tetB, and sul1) among isolates from the imported breeds (87%, 70%, and 65.2%) was higher than that in those from domestic breeds (35.2%, 36.6, and 14.1%). To our knowledge, this is the first report of ESBLs-producing Salmonella isolated from a Chinese native breed of breeder chickens. Our results also highlight that a high prevalence of multidrug-resistant Salmonella enterica contamination is widespread among different breeds of breeder chickens, which is a major risk of food-borne diseases and public health. MDPI 2023-02-03 /pmc/articles/PMC9962450/ /pubmed/36838355 http://dx.doi.org/10.3390/microorganisms11020390 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shi, Wenjian Tang, Wenli Li, Yafei Han, Yu Cui, Lulu Sun, Shuhong Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title | Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title_full | Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title_fullStr | Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title_full_unstemmed | Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title_short | Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds |
title_sort | comparative analysis between salmonella enterica isolated from imported and chinese native chicken breeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962450/ https://www.ncbi.nlm.nih.gov/pubmed/36838355 http://dx.doi.org/10.3390/microorganisms11020390 |
work_keys_str_mv | AT shiwenjian comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds AT tangwenli comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds AT liyafei comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds AT hanyu comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds AT cuilulu comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds AT sunshuhong comparativeanalysisbetweensalmonellaentericaisolatedfromimportedandchinesenativechickenbreeds |