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Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements

So far, Bacillus species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of Bacillus species as ‘natural’ probiotics and evaluate their microbiological characteristics. An indu...

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Autores principales: Łubkowska, Beata, Jeżewska-Frąckowiak, Joanna, Sroczyński, Michał, Dzitkowska-Zabielska, Magdalena, Bojarczuk, Aleksandra, Skowron, Piotr M., Cięszczyk, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962517/
https://www.ncbi.nlm.nih.gov/pubmed/36838453
http://dx.doi.org/10.3390/microorganisms11020488
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author Łubkowska, Beata
Jeżewska-Frąckowiak, Joanna
Sroczyński, Michał
Dzitkowska-Zabielska, Magdalena
Bojarczuk, Aleksandra
Skowron, Piotr M.
Cięszczyk, Paweł
author_facet Łubkowska, Beata
Jeżewska-Frąckowiak, Joanna
Sroczyński, Michał
Dzitkowska-Zabielska, Magdalena
Bojarczuk, Aleksandra
Skowron, Piotr M.
Cięszczyk, Paweł
author_sort Łubkowska, Beata
collection PubMed
description So far, Bacillus species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of Bacillus species as ‘natural’ probiotics and evaluate their microbiological characteristics. An industrially used microbiological concentrates and their components of mixed Bacillus species cultures were tested, which may be a promising bacteria source for food probiotic preparation for supplementary diet. In this study, antagonistic activities and probiotic potential of Bacillus species, derived from an industrial microbiological concentrate, were demonstrated. The cell free supernatants (CFS) from Bacillus licheniformis mostly inhibited the growth of foodborne pathogenic bacteria, such as Escherichia coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, and Staphylococcus aureus KCCM 11335, while some of Bacillus strains showed synergistic effect with foodborne pathogenic bacteria. Moreover, Bacillus strains identified by the MALDI TOF-MS method were found sensitive to chloramphenicol, kanamycin, and rifampicin. B. licheniformis and B. cereus displayed the least sensitivity to the other tested antibiotics, such as ampicillin, ampicillin and sulfbactam, streptomycin, and oxacillin and bacitracin. Furthermore, some of the bacterial species detected extended their growth range from the mesophilic to moderately thermophilic range, up to 54 °C. Thus, their potential sensitivity to thermophilic TP-84 bacteriophage, infecting thermophilic Bacilli, was tested for the purpose of isolation a new bacterial host for engineered bionanoparticles construction. We reason that the natural environmental microflora of non-pathogenic Bacillus species, especially B. licheniformis, can become a present probiotic remedy for many contemporary issues related to gastrointestinal tract health, especially for individuals under metabolic strain or for the increasingly growing group of lactose-intolerant people.
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spelling pubmed-99625172023-02-26 Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements Łubkowska, Beata Jeżewska-Frąckowiak, Joanna Sroczyński, Michał Dzitkowska-Zabielska, Magdalena Bojarczuk, Aleksandra Skowron, Piotr M. Cięszczyk, Paweł Microorganisms Article So far, Bacillus species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of Bacillus species as ‘natural’ probiotics and evaluate their microbiological characteristics. An industrially used microbiological concentrates and their components of mixed Bacillus species cultures were tested, which may be a promising bacteria source for food probiotic preparation for supplementary diet. In this study, antagonistic activities and probiotic potential of Bacillus species, derived from an industrial microbiological concentrate, were demonstrated. The cell free supernatants (CFS) from Bacillus licheniformis mostly inhibited the growth of foodborne pathogenic bacteria, such as Escherichia coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, and Staphylococcus aureus KCCM 11335, while some of Bacillus strains showed synergistic effect with foodborne pathogenic bacteria. Moreover, Bacillus strains identified by the MALDI TOF-MS method were found sensitive to chloramphenicol, kanamycin, and rifampicin. B. licheniformis and B. cereus displayed the least sensitivity to the other tested antibiotics, such as ampicillin, ampicillin and sulfbactam, streptomycin, and oxacillin and bacitracin. Furthermore, some of the bacterial species detected extended their growth range from the mesophilic to moderately thermophilic range, up to 54 °C. Thus, their potential sensitivity to thermophilic TP-84 bacteriophage, infecting thermophilic Bacilli, was tested for the purpose of isolation a new bacterial host for engineered bionanoparticles construction. We reason that the natural environmental microflora of non-pathogenic Bacillus species, especially B. licheniformis, can become a present probiotic remedy for many contemporary issues related to gastrointestinal tract health, especially for individuals under metabolic strain or for the increasingly growing group of lactose-intolerant people. MDPI 2023-02-16 /pmc/articles/PMC9962517/ /pubmed/36838453 http://dx.doi.org/10.3390/microorganisms11020488 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Łubkowska, Beata
Jeżewska-Frąckowiak, Joanna
Sroczyński, Michał
Dzitkowska-Zabielska, Magdalena
Bojarczuk, Aleksandra
Skowron, Piotr M.
Cięszczyk, Paweł
Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title_full Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title_fullStr Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title_full_unstemmed Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title_short Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements
title_sort analysis of industrial bacillus species as potential probiotics for dietary supplements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962517/
https://www.ncbi.nlm.nih.gov/pubmed/36838453
http://dx.doi.org/10.3390/microorganisms11020488
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