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Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts

HIGHLIGHTS: Ultrasonic extract of rosemary with ethanol was very rich in polyphenols. Higher antioxidant ability was observed at short time extraction (10 mins). Protective effect against oxidative stress was tested in HS-68 fibroblast cell lines. Strong protective effect was registered at low conce...

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Autores principales: Dhouibi, Nedra, Manuguerra, Simona, Arena, Rosaria, Messina, Concetta Maria, Santulli, Andrea, Kacem, Seifeddine, Dhaouadi, Hatem, Mahdhi, Abdelkarim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962555/
https://www.ncbi.nlm.nih.gov/pubmed/36837909
http://dx.doi.org/10.3390/metabo13020290
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author Dhouibi, Nedra
Manuguerra, Simona
Arena, Rosaria
Messina, Concetta Maria
Santulli, Andrea
Kacem, Seifeddine
Dhaouadi, Hatem
Mahdhi, Abdelkarim
author_facet Dhouibi, Nedra
Manuguerra, Simona
Arena, Rosaria
Messina, Concetta Maria
Santulli, Andrea
Kacem, Seifeddine
Dhaouadi, Hatem
Mahdhi, Abdelkarim
author_sort Dhouibi, Nedra
collection PubMed
description HIGHLIGHTS: Ultrasonic extract of rosemary with ethanol was very rich in polyphenols. Higher antioxidant ability was observed at short time extraction (10 mins). Protective effect against oxidative stress was tested in HS-68 fibroblast cell lines. Strong protective effect was registered at low concentrations. ABSTRACT: Rosmarinus officinalis L. is a dietary source that produces polyphenols as secondary metabolites. These natural compounds with potent antioxidant abilities are increasingly recommended as a supplement to inhibit oxidative stress. In the current work, we evaluated the impact of the extraction method on the chemical composition of R. officinalis extract, especially on the content of carnosic (CA) and rosmarinic (RA) acids using UPLC-MS-DAD as well as on their antioxidant potency. Four extracts of Tunisian rosemary were obtained from non-conventional extraction techniques:ultrasound-assisted extraction (UAE),supercritical extraction (SFE) and UAE and SFE combined ((UAE-SFE(I), UAE-SFE(II)). The UAE exhibited the best total phenolic compounds (i.e., 85.27 mg GAEg(−1)), the highest content of CAand RA and the strongest antioxidant abilities (i.e., IC(50) = 0.13 mg/mL and EC(50) = 0.93 mg/mL for DPPH scavenging test and iron reducing power ability assay). The evaluation of antioxidant activity of UAE inhuman skin fibroblast (HS-68) cell line was carried out after the induction of oxidative stress. The results determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a strong protective effect against H(2)O(2)oxidative stress induction in cells pretreated with UAE. The obtained results allow us to give new insight about the effect of the extraction method on the chemical composition and biological activities of the extract and the importance of the choice of the most appropriate processing technique to prepare rosemary extract with a high antioxidant potency and protective effect against oxidative stress.
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spelling pubmed-99625552023-02-26 Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts Dhouibi, Nedra Manuguerra, Simona Arena, Rosaria Messina, Concetta Maria Santulli, Andrea Kacem, Seifeddine Dhaouadi, Hatem Mahdhi, Abdelkarim Metabolites Article HIGHLIGHTS: Ultrasonic extract of rosemary with ethanol was very rich in polyphenols. Higher antioxidant ability was observed at short time extraction (10 mins). Protective effect against oxidative stress was tested in HS-68 fibroblast cell lines. Strong protective effect was registered at low concentrations. ABSTRACT: Rosmarinus officinalis L. is a dietary source that produces polyphenols as secondary metabolites. These natural compounds with potent antioxidant abilities are increasingly recommended as a supplement to inhibit oxidative stress. In the current work, we evaluated the impact of the extraction method on the chemical composition of R. officinalis extract, especially on the content of carnosic (CA) and rosmarinic (RA) acids using UPLC-MS-DAD as well as on their antioxidant potency. Four extracts of Tunisian rosemary were obtained from non-conventional extraction techniques:ultrasound-assisted extraction (UAE),supercritical extraction (SFE) and UAE and SFE combined ((UAE-SFE(I), UAE-SFE(II)). The UAE exhibited the best total phenolic compounds (i.e., 85.27 mg GAEg(−1)), the highest content of CAand RA and the strongest antioxidant abilities (i.e., IC(50) = 0.13 mg/mL and EC(50) = 0.93 mg/mL for DPPH scavenging test and iron reducing power ability assay). The evaluation of antioxidant activity of UAE inhuman skin fibroblast (HS-68) cell line was carried out after the induction of oxidative stress. The results determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a strong protective effect against H(2)O(2)oxidative stress induction in cells pretreated with UAE. The obtained results allow us to give new insight about the effect of the extraction method on the chemical composition and biological activities of the extract and the importance of the choice of the most appropriate processing technique to prepare rosemary extract with a high antioxidant potency and protective effect against oxidative stress. MDPI 2023-02-16 /pmc/articles/PMC9962555/ /pubmed/36837909 http://dx.doi.org/10.3390/metabo13020290 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dhouibi, Nedra
Manuguerra, Simona
Arena, Rosaria
Messina, Concetta Maria
Santulli, Andrea
Kacem, Seifeddine
Dhaouadi, Hatem
Mahdhi, Abdelkarim
Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title_full Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title_fullStr Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title_full_unstemmed Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title_short Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts
title_sort impact of the extraction method on the chemical composition and antioxidant potency of rosmarinus officinalis l. extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962555/
https://www.ncbi.nlm.nih.gov/pubmed/36837909
http://dx.doi.org/10.3390/metabo13020290
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