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Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance

Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a catastrophic disease affecting kiwifruit worldwide. As no effective cure has been developed, planting Psa-resistant cultivars is the best way to avoid bacterial canker in kiwifruit cultivation. However, the differe...

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Autores principales: Wang, Yan, Tan, Zecheng, Zhen, Xi, Liang, Yuanyuan, Gao, Jianyou, Zhao, Yanhui, Liu, Shibiao, Zha, Manrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962870/
https://www.ncbi.nlm.nih.gov/pubmed/36840266
http://dx.doi.org/10.3390/plants12040918
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author Wang, Yan
Tan, Zecheng
Zhen, Xi
Liang, Yuanyuan
Gao, Jianyou
Zhao, Yanhui
Liu, Shibiao
Zha, Manrong
author_facet Wang, Yan
Tan, Zecheng
Zhen, Xi
Liang, Yuanyuan
Gao, Jianyou
Zhao, Yanhui
Liu, Shibiao
Zha, Manrong
author_sort Wang, Yan
collection PubMed
description Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a catastrophic disease affecting kiwifruit worldwide. As no effective cure has been developed, planting Psa-resistant cultivars is the best way to avoid bacterial canker in kiwifruit cultivation. However, the differences in the mechanism of resistance between cultivars is poorly understood. In the present study, five local kiwifruit cultivars were used for Psa resistance evaluation and classified into different resistance categories, tolerant (T), susceptible (S), and highly susceptible (HS), based on their various symptoms of lesions on the cane. Susceptible and highly susceptible varieties had a higher sucrose concentration, and a greater decrease in sucrose content was observed after Psa inoculation in phloem than in tolerant varieties. Three invertase activities and their corresponding gene expressions were detected in the phloem with lesions and showed the same trends as the variations in sucrose concentration. Meanwhile, after Psa inoculation, enzyme activities involved in antioxidant defense responses, such as PAL, POD, and CAT, were also altered in the phloem of the lesion position. With no differences among cultivars, PAL and POD activities in phloem first increased and then decreased after Psa inoculation. However, great differences in CAT activities were observed between T and S/HS categories. Our results demonstrate that sucrose content was negatively correlated with the disease resistance of different cultivars and that the increase in immune response enzymes is likely caused by increased sucrose metabolism in the phloem.
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spelling pubmed-99628702023-02-26 Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance Wang, Yan Tan, Zecheng Zhen, Xi Liang, Yuanyuan Gao, Jianyou Zhao, Yanhui Liu, Shibiao Zha, Manrong Plants (Basel) Article Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a catastrophic disease affecting kiwifruit worldwide. As no effective cure has been developed, planting Psa-resistant cultivars is the best way to avoid bacterial canker in kiwifruit cultivation. However, the differences in the mechanism of resistance between cultivars is poorly understood. In the present study, five local kiwifruit cultivars were used for Psa resistance evaluation and classified into different resistance categories, tolerant (T), susceptible (S), and highly susceptible (HS), based on their various symptoms of lesions on the cane. Susceptible and highly susceptible varieties had a higher sucrose concentration, and a greater decrease in sucrose content was observed after Psa inoculation in phloem than in tolerant varieties. Three invertase activities and their corresponding gene expressions were detected in the phloem with lesions and showed the same trends as the variations in sucrose concentration. Meanwhile, after Psa inoculation, enzyme activities involved in antioxidant defense responses, such as PAL, POD, and CAT, were also altered in the phloem of the lesion position. With no differences among cultivars, PAL and POD activities in phloem first increased and then decreased after Psa inoculation. However, great differences in CAT activities were observed between T and S/HS categories. Our results demonstrate that sucrose content was negatively correlated with the disease resistance of different cultivars and that the increase in immune response enzymes is likely caused by increased sucrose metabolism in the phloem. MDPI 2023-02-17 /pmc/articles/PMC9962870/ /pubmed/36840266 http://dx.doi.org/10.3390/plants12040918 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yan
Tan, Zecheng
Zhen, Xi
Liang, Yuanyuan
Gao, Jianyou
Zhao, Yanhui
Liu, Shibiao
Zha, Manrong
Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title_full Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title_fullStr Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title_full_unstemmed Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title_short Contribution of Sucrose Metabolism in Phloem to Kiwifruit Bacterial Canker Resistance
title_sort contribution of sucrose metabolism in phloem to kiwifruit bacterial canker resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9962870/
https://www.ncbi.nlm.nih.gov/pubmed/36840266
http://dx.doi.org/10.3390/plants12040918
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