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Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease

Background: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance of these findings in humans and the roles of other viruses in the pathogenesis of CHD remain unknown. Methods: We obtained plasma samples, collected at...

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Autores principales: Garand, Mathieu, Huang, Susie S. Y., Goessling, Lisa S., Wan, Fei, Santillan, Donna A., Santillan, Mark K., Brar, Anoop, Wylie, Todd N., Wylie, Kristine M., Eghtesady, Pirooz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963073/
https://www.ncbi.nlm.nih.gov/pubmed/36838226
http://dx.doi.org/10.3390/microorganisms11020262
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author Garand, Mathieu
Huang, Susie S. Y.
Goessling, Lisa S.
Wan, Fei
Santillan, Donna A.
Santillan, Mark K.
Brar, Anoop
Wylie, Todd N.
Wylie, Kristine M.
Eghtesady, Pirooz
author_facet Garand, Mathieu
Huang, Susie S. Y.
Goessling, Lisa S.
Wan, Fei
Santillan, Donna A.
Santillan, Mark K.
Brar, Anoop
Wylie, Todd N.
Wylie, Kristine M.
Eghtesady, Pirooz
author_sort Garand, Mathieu
collection PubMed
description Background: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance of these findings in humans and the roles of other viruses in the pathogenesis of CHD remain unknown. Methods: We obtained plasma samples, collected at all trimesters, from 89 subjects (104 pregnancies), 73 healthy controls (88 pregnancies), and 16 with CHD–affected birth (16 pregnancies), from the Perinatal Family Tissue Bank (PFTB). We performed CVB IgG/IgM serological assays on plasma. We also used ViroCap sequencing and PCR to test for viral nucleic acid in plasma, circulating leukocytes from the buffy coat, and in the media of a co-culture system. Results: CVB IgG/IgM results indicated that prior exposure was 7.8 times more common in the CHD group (95% CI, 1.14–54.24, adj. p-value = 0.036). However, the CVB viral genome was not detected in plasma, buffy coat, or co-culture supernatant by molecular assays, although other viruses were detected. Conclusion: Detection of viral nucleic acid in plasma was infrequent and specifically no CVB genome was detected. However, serology demonstrated that prior CVB exposure is higher in CHD-affected pregnancies. Further studies are warranted to understand the magnitude of the contribution of the maternal blood virome to the pathogenesis of CHD.
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spelling pubmed-99630732023-02-26 Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease Garand, Mathieu Huang, Susie S. Y. Goessling, Lisa S. Wan, Fei Santillan, Donna A. Santillan, Mark K. Brar, Anoop Wylie, Todd N. Wylie, Kristine M. Eghtesady, Pirooz Microorganisms Article Background: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance of these findings in humans and the roles of other viruses in the pathogenesis of CHD remain unknown. Methods: We obtained plasma samples, collected at all trimesters, from 89 subjects (104 pregnancies), 73 healthy controls (88 pregnancies), and 16 with CHD–affected birth (16 pregnancies), from the Perinatal Family Tissue Bank (PFTB). We performed CVB IgG/IgM serological assays on plasma. We also used ViroCap sequencing and PCR to test for viral nucleic acid in plasma, circulating leukocytes from the buffy coat, and in the media of a co-culture system. Results: CVB IgG/IgM results indicated that prior exposure was 7.8 times more common in the CHD group (95% CI, 1.14–54.24, adj. p-value = 0.036). However, the CVB viral genome was not detected in plasma, buffy coat, or co-culture supernatant by molecular assays, although other viruses were detected. Conclusion: Detection of viral nucleic acid in plasma was infrequent and specifically no CVB genome was detected. However, serology demonstrated that prior CVB exposure is higher in CHD-affected pregnancies. Further studies are warranted to understand the magnitude of the contribution of the maternal blood virome to the pathogenesis of CHD. MDPI 2023-01-19 /pmc/articles/PMC9963073/ /pubmed/36838226 http://dx.doi.org/10.3390/microorganisms11020262 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garand, Mathieu
Huang, Susie S. Y.
Goessling, Lisa S.
Wan, Fei
Santillan, Donna A.
Santillan, Mark K.
Brar, Anoop
Wylie, Todd N.
Wylie, Kristine M.
Eghtesady, Pirooz
Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title_full Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title_fullStr Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title_full_unstemmed Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title_short Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
title_sort virome analysis and association of positive coxsackievirus b serology during pregnancy with congenital heart disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963073/
https://www.ncbi.nlm.nih.gov/pubmed/36838226
http://dx.doi.org/10.3390/microorganisms11020262
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