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Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique

Dengue infection has been a public health problem worldwide, especially in tropical areas. A lack of sensitive diagnostic methods in the early phase of the illness is one of the challenging problems in clinical practices. We, herein, analyzed 86 sera of acute febrile patients, from both dengue and n...

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Autores principales: Luvira, Viravarn, Thawornkuno, Charin, Lawpoolsri, Saranath, Thippornchai, Narin, Duangdee, Chatnapa, Ngamprasertchai, Thundon, Leaungwutiwong, Pornsawan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963119/
https://www.ncbi.nlm.nih.gov/pubmed/36828533
http://dx.doi.org/10.3390/tropicalmed8020117
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author Luvira, Viravarn
Thawornkuno, Charin
Lawpoolsri, Saranath
Thippornchai, Narin
Duangdee, Chatnapa
Ngamprasertchai, Thundon
Leaungwutiwong, Pornsawan
author_facet Luvira, Viravarn
Thawornkuno, Charin
Lawpoolsri, Saranath
Thippornchai, Narin
Duangdee, Chatnapa
Ngamprasertchai, Thundon
Leaungwutiwong, Pornsawan
author_sort Luvira, Viravarn
collection PubMed
description Dengue infection has been a public health problem worldwide, especially in tropical areas. A lack of sensitive diagnostic methods in the early phase of the illness is one of the challenging problems in clinical practices. We, herein, analyzed 86 sera of acute febrile patients, from both dengue and non-dengue febrile illness, to study the diagnostic performance of dengue diagnostics. When compared with detection by Polymerase Chain Reaction (PCR), dengue NS1 detection by enzyme-linked immunosorbent assay (ELISA) had the highest sensitivity of 82.4% (with 94.3% specificity), while NS1 by rapid diagnostic test (RDT) had 76.5% sensitivity. IgM detection by ELISA and RDT showed only 27.5% and 17.9% sensitivity, respectively. The combination of NS1 and IgM in RDT yielded a sensitivity of 78.4%, with 97.1% specificity. One of the essential steps in making a diagnosis from patient samples is the preparation process. At present, a variety of techniques have been used to increase the number of analytes in clinical samples. In this study, we focused on the sample concentration method. The sera were concentrated three times with the ultrafiltration method using a 10 kDa molecular weight cut-off membrane. The results showed an increase in the sensitivity of RDT-NS1 detection at 80.4%, with 100% specificity. When combining NS1 and IgM detection, the concentration method granted RDT an 82.4% sensitivity, with 100% specificity. In conclusion, serum concentration by the ultrafiltration method is a simple and applicable technique. It could increase the diagnostic performance of point-of-care dengue diagnostics.
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spelling pubmed-99631192023-02-26 Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique Luvira, Viravarn Thawornkuno, Charin Lawpoolsri, Saranath Thippornchai, Narin Duangdee, Chatnapa Ngamprasertchai, Thundon Leaungwutiwong, Pornsawan Trop Med Infect Dis Article Dengue infection has been a public health problem worldwide, especially in tropical areas. A lack of sensitive diagnostic methods in the early phase of the illness is one of the challenging problems in clinical practices. We, herein, analyzed 86 sera of acute febrile patients, from both dengue and non-dengue febrile illness, to study the diagnostic performance of dengue diagnostics. When compared with detection by Polymerase Chain Reaction (PCR), dengue NS1 detection by enzyme-linked immunosorbent assay (ELISA) had the highest sensitivity of 82.4% (with 94.3% specificity), while NS1 by rapid diagnostic test (RDT) had 76.5% sensitivity. IgM detection by ELISA and RDT showed only 27.5% and 17.9% sensitivity, respectively. The combination of NS1 and IgM in RDT yielded a sensitivity of 78.4%, with 97.1% specificity. One of the essential steps in making a diagnosis from patient samples is the preparation process. At present, a variety of techniques have been used to increase the number of analytes in clinical samples. In this study, we focused on the sample concentration method. The sera were concentrated three times with the ultrafiltration method using a 10 kDa molecular weight cut-off membrane. The results showed an increase in the sensitivity of RDT-NS1 detection at 80.4%, with 100% specificity. When combining NS1 and IgM detection, the concentration method granted RDT an 82.4% sensitivity, with 100% specificity. In conclusion, serum concentration by the ultrafiltration method is a simple and applicable technique. It could increase the diagnostic performance of point-of-care dengue diagnostics. MDPI 2023-02-14 /pmc/articles/PMC9963119/ /pubmed/36828533 http://dx.doi.org/10.3390/tropicalmed8020117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luvira, Viravarn
Thawornkuno, Charin
Lawpoolsri, Saranath
Thippornchai, Narin
Duangdee, Chatnapa
Ngamprasertchai, Thundon
Leaungwutiwong, Pornsawan
Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title_full Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title_fullStr Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title_full_unstemmed Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title_short Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
title_sort diagnostic performance of dengue ns1 and antibodies by serum concentration technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963119/
https://www.ncbi.nlm.nih.gov/pubmed/36828533
http://dx.doi.org/10.3390/tropicalmed8020117
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