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Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth
Cervicovaginal fluid (CVF) is an excellent specimen for monitoring preterm birth (PTB) as it characterizes cervical metabolites, the vaginal environment, and specific host immune responses. However, extensive lipid analysis of CVF to explain PTB has not been studied. In this study, we performed a sy...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963162/ https://www.ncbi.nlm.nih.gov/pubmed/36837795 http://dx.doi.org/10.3390/metabo13020177 |
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author | Hong, Se Hee Lee, Ji-Youn Seo, Sumin Shin, Bohyun Jeong, Cho Hee Bae, Eunbin Kim, Jiyu Lee, Donghee An, Byungchan Shim, Minki Shin, Jung Hoon Lee, Dong-Kyu Kim, Young Ju Han, Sang Beom |
author_facet | Hong, Se Hee Lee, Ji-Youn Seo, Sumin Shin, Bohyun Jeong, Cho Hee Bae, Eunbin Kim, Jiyu Lee, Donghee An, Byungchan Shim, Minki Shin, Jung Hoon Lee, Dong-Kyu Kim, Young Ju Han, Sang Beom |
author_sort | Hong, Se Hee |
collection | PubMed |
description | Cervicovaginal fluid (CVF) is an excellent specimen for monitoring preterm birth (PTB) as it characterizes cervical metabolites, the vaginal environment, and specific host immune responses. However, extensive lipid analysis of CVF to explain PTB has not been studied. In this study, we performed a systematic analysis combining high-throughput lipid analysis and omics to discover the unique metabolic properties of the cervix. Liquid chromatography-high resolution mass spectrometry successfully detected a total of 190 lipids in the CVF of 30 PTB and 30 term birth (TB) pregnant women. The whole lipidomics dataset analyzed by combining multivariate and univariate statistical analysis revealed 35 lipid biomarkers, including phospholipids and sphingolipids. Remarkably, sphingomyelin, which plays a physiologically essential role in sphingolipids, was significantly downregulated in PTB. Metabolic pathway study provides a close relationship between vaginal microbial organization and cell membrane formation, further supporting the robustness of our findings. Sphingolipids and phospholipids, which were determined to be important lipids for predicting PTB in our study, showed a high value of receiver operating characteristic (ROC) curve >0.7, indicating that a lipid diagnostic test and understanding the mechanism of lipids is highly related to the vaginal microbiome. Therefore, our result has high potential as a predictor of PTB. |
format | Online Article Text |
id | pubmed-9963162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99631622023-02-26 Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth Hong, Se Hee Lee, Ji-Youn Seo, Sumin Shin, Bohyun Jeong, Cho Hee Bae, Eunbin Kim, Jiyu Lee, Donghee An, Byungchan Shim, Minki Shin, Jung Hoon Lee, Dong-Kyu Kim, Young Ju Han, Sang Beom Metabolites Article Cervicovaginal fluid (CVF) is an excellent specimen for monitoring preterm birth (PTB) as it characterizes cervical metabolites, the vaginal environment, and specific host immune responses. However, extensive lipid analysis of CVF to explain PTB has not been studied. In this study, we performed a systematic analysis combining high-throughput lipid analysis and omics to discover the unique metabolic properties of the cervix. Liquid chromatography-high resolution mass spectrometry successfully detected a total of 190 lipids in the CVF of 30 PTB and 30 term birth (TB) pregnant women. The whole lipidomics dataset analyzed by combining multivariate and univariate statistical analysis revealed 35 lipid biomarkers, including phospholipids and sphingolipids. Remarkably, sphingomyelin, which plays a physiologically essential role in sphingolipids, was significantly downregulated in PTB. Metabolic pathway study provides a close relationship between vaginal microbial organization and cell membrane formation, further supporting the robustness of our findings. Sphingolipids and phospholipids, which were determined to be important lipids for predicting PTB in our study, showed a high value of receiver operating characteristic (ROC) curve >0.7, indicating that a lipid diagnostic test and understanding the mechanism of lipids is highly related to the vaginal microbiome. Therefore, our result has high potential as a predictor of PTB. MDPI 2023-01-25 /pmc/articles/PMC9963162/ /pubmed/36837795 http://dx.doi.org/10.3390/metabo13020177 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hong, Se Hee Lee, Ji-Youn Seo, Sumin Shin, Bohyun Jeong, Cho Hee Bae, Eunbin Kim, Jiyu Lee, Donghee An, Byungchan Shim, Minki Shin, Jung Hoon Lee, Dong-Kyu Kim, Young Ju Han, Sang Beom Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title | Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title_full | Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title_fullStr | Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title_full_unstemmed | Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title_short | Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth |
title_sort | lipidomic analysis of cervicovaginal fluid for elucidating prognostic biomarkers and relevant phospholipid and sphingolipid pathways in preterm birth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963162/ https://www.ncbi.nlm.nih.gov/pubmed/36837795 http://dx.doi.org/10.3390/metabo13020177 |
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