Cargando…
Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway
MicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after Rhizoctonia solani (R. solani) infection....
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963240/ https://www.ncbi.nlm.nih.gov/pubmed/36835070 http://dx.doi.org/10.3390/ijms24043653 |
_version_ | 1784896204104007680 |
---|---|
author | Feng, Tao Zhang, Zhao-Yang Gao, Peng Feng, Zhi-Ming Zuo, Shi-Min Ouyang, Shou-Qiang |
author_facet | Feng, Tao Zhang, Zhao-Yang Gao, Peng Feng, Zhi-Ming Zuo, Shi-Min Ouyang, Shou-Qiang |
author_sort | Feng, Tao |
collection | PubMed |
description | MicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after Rhizoctonia solani (R. solani) infection. In order to clarify the function of Osa-miR444b.2 responding to R. solani infection in rice, transgenic lines over-expressing and knocking out Osa-miR444b.2 were generated in the background of susceptible cultivar Xu3 and resistant cultivar YSBR1, respectively. Over-expressing Osa-miR444b.2 resulted in compromised resistance to R. solani. In contrast, the knocking out Osa-miR444b.2 lines exhibited improved resistance to R. solani. Furthermore, knocking out Osa-miR444b.2 resulted in increased height, tillers, smaller panicle, and decreased 1000-grain weight and primary branches. However, the transgenic lines over-expressing Osa-miR444b.2 showed decreased primary branches and tillers, but increased panicle length. These results indicated that Osa-miR444b.2 was also involved in regulating the agronomic traits in rice. The RNA-seq assay revealed that Osa-miR444b.2 mainly regulated the resistance to rice sheath blight disease by affecting the expression of plant hormone signaling pathways-related genes such as ET and IAA, and transcription factors such as WRKYs and F-boxes. Together, our results suggest that Osa-miR444b.2 negatively mediated the resistance to R. solani in rice, which will contribute to the cultivation of sheath blight resistant varieties. |
format | Online Article Text |
id | pubmed-9963240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99632402023-02-26 Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway Feng, Tao Zhang, Zhao-Yang Gao, Peng Feng, Zhi-Ming Zuo, Shi-Min Ouyang, Shou-Qiang Int J Mol Sci Article MicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after Rhizoctonia solani (R. solani) infection. In order to clarify the function of Osa-miR444b.2 responding to R. solani infection in rice, transgenic lines over-expressing and knocking out Osa-miR444b.2 were generated in the background of susceptible cultivar Xu3 and resistant cultivar YSBR1, respectively. Over-expressing Osa-miR444b.2 resulted in compromised resistance to R. solani. In contrast, the knocking out Osa-miR444b.2 lines exhibited improved resistance to R. solani. Furthermore, knocking out Osa-miR444b.2 resulted in increased height, tillers, smaller panicle, and decreased 1000-grain weight and primary branches. However, the transgenic lines over-expressing Osa-miR444b.2 showed decreased primary branches and tillers, but increased panicle length. These results indicated that Osa-miR444b.2 was also involved in regulating the agronomic traits in rice. The RNA-seq assay revealed that Osa-miR444b.2 mainly regulated the resistance to rice sheath blight disease by affecting the expression of plant hormone signaling pathways-related genes such as ET and IAA, and transcription factors such as WRKYs and F-boxes. Together, our results suggest that Osa-miR444b.2 negatively mediated the resistance to R. solani in rice, which will contribute to the cultivation of sheath blight resistant varieties. MDPI 2023-02-11 /pmc/articles/PMC9963240/ /pubmed/36835070 http://dx.doi.org/10.3390/ijms24043653 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feng, Tao Zhang, Zhao-Yang Gao, Peng Feng, Zhi-Ming Zuo, Shi-Min Ouyang, Shou-Qiang Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title_full | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title_fullStr | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title_full_unstemmed | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title_short | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
title_sort | suppression of rice osa-mir444.2 improves the resistance to sheath blight in rice mediating through the phytohormone pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963240/ https://www.ncbi.nlm.nih.gov/pubmed/36835070 http://dx.doi.org/10.3390/ijms24043653 |
work_keys_str_mv | AT fengtao suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway AT zhangzhaoyang suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway AT gaopeng suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway AT fengzhiming suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway AT zuoshimin suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway AT ouyangshouqiang suppressionofriceosamir4442improvestheresistancetosheathblightinricemediatingthroughthephytohormonepathway |