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New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics
Plant growth-promoting endophytic microbes have drawn the attention of researchers owing to their ability to confer fitness benefits in many plant species. Here, we report agriculturally beneficial traits of rice-leaf-adapted endophytic Microbacterium testaceum. Our polyphasic taxonomic investigatio...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963279/ https://www.ncbi.nlm.nih.gov/pubmed/36838327 http://dx.doi.org/10.3390/microorganisms11020362 |
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author | Patel, Asharani Sahu, Kuleshwar Prasad Mehta, Sahil Javed, Mohammed Balamurugan, Alexander Ashajyothi, Mushineni Sheoran, Neelam Ganesan, Prakash Kundu, Aditi Gopalakrishnan, Subbaiyan Gogoi, Robin Kumar, Aundy |
author_facet | Patel, Asharani Sahu, Kuleshwar Prasad Mehta, Sahil Javed, Mohammed Balamurugan, Alexander Ashajyothi, Mushineni Sheoran, Neelam Ganesan, Prakash Kundu, Aditi Gopalakrishnan, Subbaiyan Gogoi, Robin Kumar, Aundy |
author_sort | Patel, Asharani |
collection | PubMed |
description | Plant growth-promoting endophytic microbes have drawn the attention of researchers owing to their ability to confer fitness benefits in many plant species. Here, we report agriculturally beneficial traits of rice-leaf-adapted endophytic Microbacterium testaceum. Our polyphasic taxonomic investigations revealed its identity as M. testaceum. The bacterium displayed typical endophytism in rice leaves, indicated by the green fluorescence of GFP-tagged M. testaceum in confocal laser scanning microscopy. Furthermore, the bacterium showed mineral solubilization and production of IAA, ammonia, and hydrolytic enzymes. Tobacco leaf infiltration assay confirmed its non-pathogenic nature on plants. The bacterium showed antifungal activity on Magnaporthe oryzae, as exemplified by secreted and volatile organic metabolome-mediated mycelial growth inhibition. GC-MS analysis of the volatilome of M. testaceum indicated the abundance of antimicrobial compounds. Bacterization of rice seedlings showed phenotypic traits of MAMP-triggered immunity (MTI), over-expression of OsNPR1 and OsCERK, and the consequent blast suppressive activity. Strikingly, M. testaceum induced the transcriptional tradeoff between physiological growth and host defense pathways as indicated by up- and downregulated DEGs. Coupled with its plant probiotic features and the defense elicitation activity, the present study paves the way for developing Microbacterium testaceum-mediated bioformulation for sustainably managing rice blast disease. |
format | Online Article Text |
id | pubmed-9963279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99632792023-02-26 New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics Patel, Asharani Sahu, Kuleshwar Prasad Mehta, Sahil Javed, Mohammed Balamurugan, Alexander Ashajyothi, Mushineni Sheoran, Neelam Ganesan, Prakash Kundu, Aditi Gopalakrishnan, Subbaiyan Gogoi, Robin Kumar, Aundy Microorganisms Article Plant growth-promoting endophytic microbes have drawn the attention of researchers owing to their ability to confer fitness benefits in many plant species. Here, we report agriculturally beneficial traits of rice-leaf-adapted endophytic Microbacterium testaceum. Our polyphasic taxonomic investigations revealed its identity as M. testaceum. The bacterium displayed typical endophytism in rice leaves, indicated by the green fluorescence of GFP-tagged M. testaceum in confocal laser scanning microscopy. Furthermore, the bacterium showed mineral solubilization and production of IAA, ammonia, and hydrolytic enzymes. Tobacco leaf infiltration assay confirmed its non-pathogenic nature on plants. The bacterium showed antifungal activity on Magnaporthe oryzae, as exemplified by secreted and volatile organic metabolome-mediated mycelial growth inhibition. GC-MS analysis of the volatilome of M. testaceum indicated the abundance of antimicrobial compounds. Bacterization of rice seedlings showed phenotypic traits of MAMP-triggered immunity (MTI), over-expression of OsNPR1 and OsCERK, and the consequent blast suppressive activity. Strikingly, M. testaceum induced the transcriptional tradeoff between physiological growth and host defense pathways as indicated by up- and downregulated DEGs. Coupled with its plant probiotic features and the defense elicitation activity, the present study paves the way for developing Microbacterium testaceum-mediated bioformulation for sustainably managing rice blast disease. MDPI 2023-01-31 /pmc/articles/PMC9963279/ /pubmed/36838327 http://dx.doi.org/10.3390/microorganisms11020362 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Patel, Asharani Sahu, Kuleshwar Prasad Mehta, Sahil Javed, Mohammed Balamurugan, Alexander Ashajyothi, Mushineni Sheoran, Neelam Ganesan, Prakash Kundu, Aditi Gopalakrishnan, Subbaiyan Gogoi, Robin Kumar, Aundy New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title_full | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title_fullStr | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title_full_unstemmed | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title_short | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
title_sort | new insights on endophytic microbacterium-assisted blast disease suppression and growth promotion in rice: revelation by polyphasic functional characterization and transcriptomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963279/ https://www.ncbi.nlm.nih.gov/pubmed/36838327 http://dx.doi.org/10.3390/microorganisms11020362 |
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