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Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin

To study the distribution features of microorganisms in distinct hydrological areas of the southern Qinshui Basin, C-N-S microorganisms were studied using 16S RNA sequencing, metagenome sequencing and geochemical technologies, showing the high sensitivity of microorganisms to the hydrodynamic dynami...

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Autores principales: Shi, Wei, Tang, Shuheng, Zhang, Songhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963281/
https://www.ncbi.nlm.nih.gov/pubmed/36838462
http://dx.doi.org/10.3390/microorganisms11020497
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author Shi, Wei
Tang, Shuheng
Zhang, Songhang
author_facet Shi, Wei
Tang, Shuheng
Zhang, Songhang
author_sort Shi, Wei
collection PubMed
description To study the distribution features of microorganisms in distinct hydrological areas of the southern Qinshui Basin, C-N-S microorganisms were studied using 16S RNA sequencing, metagenome sequencing and geochemical technologies, showing the high sensitivity of microorganisms to the hydrodynamic dynamics of coal. The hydrodynamic intensity of the #3 coal gradually decreased from the runoff areas to the stagnant areas. The stagnant zones have higher reservoir pressure, methane content, δ(13)C(DIC) and TDS and lower SO(4)(2−), Fe(3+) and NO(3)(−) concentrations than the runoff areas. C-N-S-cycling microorganisms, including those engaged in methanogenesis, nitrate respiration, fermentation, nitrate reduction, dark oxidation of sulfur compounds, sulfate respiration, iron respiration, chlorate reduction, aromatic compound degradation, denitrification, ammonification and nitrogen fixation, were more abundant in the stagnant areas. The relative abundance of C-N-S functional genes, including genes related to C metabolism (e.g., mcr, mer, mtr, fwd and mtd), N metabolism (e.g., nifDKH, nirK, narGHI, nosZ, amoB, norC and napAB) and sulfur metabolism (e.g., dsrAB and PAPSS), increased in the stagnant zones, indicating that there was active microbiological C-N-S cycling in the stagnant areas. The degradation and fermentation of terrestrial plant organic carbon and coal seam organic matter could provide substrates for methanogens, while nitrogen fixation and nitrification can provide nitrogen for methanogens, which are all favorable factors for stronger methanogenesis in stagnant areas. The coal in the study area is currently in the secondary biogenic gas generation stage because of the rising of the strata, which recharges atmospheric precipitation. The random forest model shows that the abundance of C-N-S microorganisms and genes could be used to distinguish different hydrological zones in coal reservoirs. Since stagnant zones are usually high-gas-bearing zones and high-production areas of CBM exploration, these microbiological indicators can be used as effective parameters to identify high-production-potential zones. In addition, nitrate respiration and sulfate respiration microorganisms consumed NO(3)(−) and SO(4)(2−), causing a decrease in the content of these two ions in the stagnant areas.
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spelling pubmed-99632812023-02-26 Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin Shi, Wei Tang, Shuheng Zhang, Songhang Microorganisms Article To study the distribution features of microorganisms in distinct hydrological areas of the southern Qinshui Basin, C-N-S microorganisms were studied using 16S RNA sequencing, metagenome sequencing and geochemical technologies, showing the high sensitivity of microorganisms to the hydrodynamic dynamics of coal. The hydrodynamic intensity of the #3 coal gradually decreased from the runoff areas to the stagnant areas. The stagnant zones have higher reservoir pressure, methane content, δ(13)C(DIC) and TDS and lower SO(4)(2−), Fe(3+) and NO(3)(−) concentrations than the runoff areas. C-N-S-cycling microorganisms, including those engaged in methanogenesis, nitrate respiration, fermentation, nitrate reduction, dark oxidation of sulfur compounds, sulfate respiration, iron respiration, chlorate reduction, aromatic compound degradation, denitrification, ammonification and nitrogen fixation, were more abundant in the stagnant areas. The relative abundance of C-N-S functional genes, including genes related to C metabolism (e.g., mcr, mer, mtr, fwd and mtd), N metabolism (e.g., nifDKH, nirK, narGHI, nosZ, amoB, norC and napAB) and sulfur metabolism (e.g., dsrAB and PAPSS), increased in the stagnant zones, indicating that there was active microbiological C-N-S cycling in the stagnant areas. The degradation and fermentation of terrestrial plant organic carbon and coal seam organic matter could provide substrates for methanogens, while nitrogen fixation and nitrification can provide nitrogen for methanogens, which are all favorable factors for stronger methanogenesis in stagnant areas. The coal in the study area is currently in the secondary biogenic gas generation stage because of the rising of the strata, which recharges atmospheric precipitation. The random forest model shows that the abundance of C-N-S microorganisms and genes could be used to distinguish different hydrological zones in coal reservoirs. Since stagnant zones are usually high-gas-bearing zones and high-production areas of CBM exploration, these microbiological indicators can be used as effective parameters to identify high-production-potential zones. In addition, nitrate respiration and sulfate respiration microorganisms consumed NO(3)(−) and SO(4)(2−), causing a decrease in the content of these two ions in the stagnant areas. MDPI 2023-02-16 /pmc/articles/PMC9963281/ /pubmed/36838462 http://dx.doi.org/10.3390/microorganisms11020497 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Wei
Tang, Shuheng
Zhang, Songhang
Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title_full Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title_fullStr Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title_full_unstemmed Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title_short Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin
title_sort microbiome of high-rank coal reservoirs in the high-production areas of the southern qinshui basin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963281/
https://www.ncbi.nlm.nih.gov/pubmed/36838462
http://dx.doi.org/10.3390/microorganisms11020497
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