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Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli

In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycl...

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Autores principales: Santos-Beneit, Fernando, Chen, Le Min, Bordel, Sergio, Frutos de la Flor, Raquel, García-Depraect, Octavio, Lebrero, Raquel, Rodriguez-Vega, Sara, Muñoz, Raúl, Börner, Rosa Aragão, Börner, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963400/
https://www.ncbi.nlm.nih.gov/pubmed/36838293
http://dx.doi.org/10.3390/microorganisms11020328
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author Santos-Beneit, Fernando
Chen, Le Min
Bordel, Sergio
Frutos de la Flor, Raquel
García-Depraect, Octavio
Lebrero, Raquel
Rodriguez-Vega, Sara
Muñoz, Raúl
Börner, Rosa Aragão
Börner, Tim
author_facet Santos-Beneit, Fernando
Chen, Le Min
Bordel, Sergio
Frutos de la Flor, Raquel
García-Depraect, Octavio
Lebrero, Raquel
Rodriguez-Vega, Sara
Muñoz, Raúl
Börner, Rosa Aragão
Börner, Tim
author_sort Santos-Beneit, Fernando
collection PubMed
description In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium.
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spelling pubmed-99634002023-02-26 Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli Santos-Beneit, Fernando Chen, Le Min Bordel, Sergio Frutos de la Flor, Raquel García-Depraect, Octavio Lebrero, Raquel Rodriguez-Vega, Sara Muñoz, Raúl Börner, Rosa Aragão Börner, Tim Microorganisms Article In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium. MDPI 2023-01-28 /pmc/articles/PMC9963400/ /pubmed/36838293 http://dx.doi.org/10.3390/microorganisms11020328 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santos-Beneit, Fernando
Chen, Le Min
Bordel, Sergio
Frutos de la Flor, Raquel
García-Depraect, Octavio
Lebrero, Raquel
Rodriguez-Vega, Sara
Muñoz, Raúl
Börner, Rosa Aragão
Börner, Tim
Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title_full Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title_fullStr Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title_full_unstemmed Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title_short Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
title_sort screening enzymes that can depolymerize commercial biodegradable polymers: heterologous expression of fusarium solani cutinase in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963400/
https://www.ncbi.nlm.nih.gov/pubmed/36838293
http://dx.doi.org/10.3390/microorganisms11020328
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