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Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli
In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycl...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963400/ https://www.ncbi.nlm.nih.gov/pubmed/36838293 http://dx.doi.org/10.3390/microorganisms11020328 |
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author | Santos-Beneit, Fernando Chen, Le Min Bordel, Sergio Frutos de la Flor, Raquel García-Depraect, Octavio Lebrero, Raquel Rodriguez-Vega, Sara Muñoz, Raúl Börner, Rosa Aragão Börner, Tim |
author_facet | Santos-Beneit, Fernando Chen, Le Min Bordel, Sergio Frutos de la Flor, Raquel García-Depraect, Octavio Lebrero, Raquel Rodriguez-Vega, Sara Muñoz, Raúl Börner, Rosa Aragão Börner, Tim |
author_sort | Santos-Beneit, Fernando |
collection | PubMed |
description | In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium. |
format | Online Article Text |
id | pubmed-9963400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99634002023-02-26 Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli Santos-Beneit, Fernando Chen, Le Min Bordel, Sergio Frutos de la Flor, Raquel García-Depraect, Octavio Lebrero, Raquel Rodriguez-Vega, Sara Muñoz, Raúl Börner, Rosa Aragão Börner, Tim Microorganisms Article In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium. MDPI 2023-01-28 /pmc/articles/PMC9963400/ /pubmed/36838293 http://dx.doi.org/10.3390/microorganisms11020328 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santos-Beneit, Fernando Chen, Le Min Bordel, Sergio Frutos de la Flor, Raquel García-Depraect, Octavio Lebrero, Raquel Rodriguez-Vega, Sara Muñoz, Raúl Börner, Rosa Aragão Börner, Tim Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title | Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title_full | Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title_fullStr | Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title_full_unstemmed | Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title_short | Screening Enzymes That Can Depolymerize Commercial Biodegradable Polymers: Heterologous Expression of Fusarium solani Cutinase in Escherichia coli |
title_sort | screening enzymes that can depolymerize commercial biodegradable polymers: heterologous expression of fusarium solani cutinase in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963400/ https://www.ncbi.nlm.nih.gov/pubmed/36838293 http://dx.doi.org/10.3390/microorganisms11020328 |
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