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On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography

Synchrotron-based soft X-ray tomography (SXT), providing three-dimensional morphology and quantitative distribution of linear absorption coefficient (LAC) of the imaged objects, is widely used in many fields to obtain ultra-structure images, especially in cellular imaging. Off-line fluorescence micr...

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Detalles Bibliográficos
Autores principales: Zhang, Chao, Wu, Zhao, Dang, Zheng, Tian, Lijiao, Guan, Yong, Liu, Gang, Tian, Yangchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963497/
https://www.ncbi.nlm.nih.gov/pubmed/36838026
http://dx.doi.org/10.3390/mi14020326
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author Zhang, Chao
Wu, Zhao
Dang, Zheng
Tian, Lijiao
Guan, Yong
Liu, Gang
Tian, Yangchao
author_facet Zhang, Chao
Wu, Zhao
Dang, Zheng
Tian, Lijiao
Guan, Yong
Liu, Gang
Tian, Yangchao
author_sort Zhang, Chao
collection PubMed
description Synchrotron-based soft X-ray tomography (SXT), providing three-dimensional morphology and quantitative distribution of linear absorption coefficient (LAC) of the imaged objects, is widely used in many fields to obtain ultra-structure images, especially in cellular imaging. Off-line fluorescence microscopies (FMs) are combined to identify the type of organelles and status of cells. However, deformation and displacement usually occur during the transfer and loading process, which decreases the precision of two-modal images’ registration. In this paper, we report on an on-line FM, at the SXT station (BL07W) of the National Synchrotron Radiation Laboratory (NSRL), which avoids deformation and displacement. Therefore, researchers can easily find the sample and take the useful data without tedious post-processing. Combining SXT with on-line FM, we achieved the identification and high-resolution imaging of an apoptotic cell. The experiments revealed that the LAC of the nucleus of the apoptotic cell was larger than that of a normal cell, which could be explained by nucleus pyknosis of the apoptotic cell.
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spelling pubmed-99634972023-02-26 On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography Zhang, Chao Wu, Zhao Dang, Zheng Tian, Lijiao Guan, Yong Liu, Gang Tian, Yangchao Micromachines (Basel) Article Synchrotron-based soft X-ray tomography (SXT), providing three-dimensional morphology and quantitative distribution of linear absorption coefficient (LAC) of the imaged objects, is widely used in many fields to obtain ultra-structure images, especially in cellular imaging. Off-line fluorescence microscopies (FMs) are combined to identify the type of organelles and status of cells. However, deformation and displacement usually occur during the transfer and loading process, which decreases the precision of two-modal images’ registration. In this paper, we report on an on-line FM, at the SXT station (BL07W) of the National Synchrotron Radiation Laboratory (NSRL), which avoids deformation and displacement. Therefore, researchers can easily find the sample and take the useful data without tedious post-processing. Combining SXT with on-line FM, we achieved the identification and high-resolution imaging of an apoptotic cell. The experiments revealed that the LAC of the nucleus of the apoptotic cell was larger than that of a normal cell, which could be explained by nucleus pyknosis of the apoptotic cell. MDPI 2023-01-27 /pmc/articles/PMC9963497/ /pubmed/36838026 http://dx.doi.org/10.3390/mi14020326 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Chao
Wu, Zhao
Dang, Zheng
Tian, Lijiao
Guan, Yong
Liu, Gang
Tian, Yangchao
On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title_full On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title_fullStr On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title_full_unstemmed On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title_short On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
title_sort on-line fluorescence microscopy for identification and imaging of apoptotic cell with synchrotron-based soft x-ray tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963497/
https://www.ncbi.nlm.nih.gov/pubmed/36838026
http://dx.doi.org/10.3390/mi14020326
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