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GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway

Short-chain fatty acids (SCFAs) play a pivotal role in regulating the proliferation and development of bovine rumen epithelial cells (BRECs). G protein-coupled receptor 41 (GPR41) is involved in the signal transduction in BRECs as a receptor for SCFAs. Nevertheless, the impact of GPR41 on the prolif...

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Autores principales: Meng, Zitong, Tan, Dejin, Cheng, Zhiqiang, Jiang, Maocheng, Zhan, Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963637/
https://www.ncbi.nlm.nih.gov/pubmed/36835615
http://dx.doi.org/10.3390/ijms24044203
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author Meng, Zitong
Tan, Dejin
Cheng, Zhiqiang
Jiang, Maocheng
Zhan, Kang
author_facet Meng, Zitong
Tan, Dejin
Cheng, Zhiqiang
Jiang, Maocheng
Zhan, Kang
author_sort Meng, Zitong
collection PubMed
description Short-chain fatty acids (SCFAs) play a pivotal role in regulating the proliferation and development of bovine rumen epithelial cells (BRECs). G protein-coupled receptor 41 (GPR41) is involved in the signal transduction in BRECs as a receptor for SCFAs. Nevertheless, the impact of GPR41 on the proliferation of BRECs has not been reported. The results of this research showed that the knockdown of GPR41 (GRP41KD) decreased BRECs proliferation compared with the wild-type BRECs (WT) (p < 0.001). The RNA sequencing (RNA-seq) analysis showed that the gene expression profiles differed between WT and GPR41KD BRECs, with the major differential genes enriched in phosphatidylinositol 3-kinase (PIK3) signaling, cell cycle, and amino acid transport pathways (p < 0.05). The transcriptome data were further validated by Western blot and qRT-PCR. It was evident that the GPR41KD BRECs downregulated the level of the PIK3-Protein kinase B (AKT)-mammalian target of the rapamycin (mTOR) signaling pathway core genes, such as PIK3, AKT, eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and mTOR contrasted with the WT cells (p < 0.01). Furthermore, the GPR41KD BRECs downregulated the level of Cyclin D2 p < 0.001) and Cyclin E2 (p < 0.05) compared with the WT cells. Therefore, it was proposed that GPR41 may affect the proliferation of BRECs by mediating the PIK3-AKT-mTOR signaling pathway.
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spelling pubmed-99636372023-02-26 GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway Meng, Zitong Tan, Dejin Cheng, Zhiqiang Jiang, Maocheng Zhan, Kang Int J Mol Sci Article Short-chain fatty acids (SCFAs) play a pivotal role in regulating the proliferation and development of bovine rumen epithelial cells (BRECs). G protein-coupled receptor 41 (GPR41) is involved in the signal transduction in BRECs as a receptor for SCFAs. Nevertheless, the impact of GPR41 on the proliferation of BRECs has not been reported. The results of this research showed that the knockdown of GPR41 (GRP41KD) decreased BRECs proliferation compared with the wild-type BRECs (WT) (p < 0.001). The RNA sequencing (RNA-seq) analysis showed that the gene expression profiles differed between WT and GPR41KD BRECs, with the major differential genes enriched in phosphatidylinositol 3-kinase (PIK3) signaling, cell cycle, and amino acid transport pathways (p < 0.05). The transcriptome data were further validated by Western blot and qRT-PCR. It was evident that the GPR41KD BRECs downregulated the level of the PIK3-Protein kinase B (AKT)-mammalian target of the rapamycin (mTOR) signaling pathway core genes, such as PIK3, AKT, eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and mTOR contrasted with the WT cells (p < 0.01). Furthermore, the GPR41KD BRECs downregulated the level of Cyclin D2 p < 0.001) and Cyclin E2 (p < 0.05) compared with the WT cells. Therefore, it was proposed that GPR41 may affect the proliferation of BRECs by mediating the PIK3-AKT-mTOR signaling pathway. MDPI 2023-02-20 /pmc/articles/PMC9963637/ /pubmed/36835615 http://dx.doi.org/10.3390/ijms24044203 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meng, Zitong
Tan, Dejin
Cheng, Zhiqiang
Jiang, Maocheng
Zhan, Kang
GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title_full GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title_fullStr GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title_full_unstemmed GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title_short GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway
title_sort gpr41 regulates the proliferation of brecs via the pik3-akt-mtor pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963637/
https://www.ncbi.nlm.nih.gov/pubmed/36835615
http://dx.doi.org/10.3390/ijms24044203
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