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Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection

Rice blast, caused by Pyricularia oryzae, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (Pi) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elit...

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Autores principales: Zampieri, Elisa, Volante, Andrea, Marè, Caterina, Orasen, Gabriele, Desiderio, Francesca, Biselli, Chiara, Canella, Marco, Carmagnola, Lorena, Milazzo, Joëlle, Adreit, Henri, Tharreau, Didier, Poncelet, Nicolas, Vaccino, Patrizia, Valè, Giampiero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963729/
https://www.ncbi.nlm.nih.gov/pubmed/36840105
http://dx.doi.org/10.3390/plants12040757
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author Zampieri, Elisa
Volante, Andrea
Marè, Caterina
Orasen, Gabriele
Desiderio, Francesca
Biselli, Chiara
Canella, Marco
Carmagnola, Lorena
Milazzo, Joëlle
Adreit, Henri
Tharreau, Didier
Poncelet, Nicolas
Vaccino, Patrizia
Valè, Giampiero
author_facet Zampieri, Elisa
Volante, Andrea
Marè, Caterina
Orasen, Gabriele
Desiderio, Francesca
Biselli, Chiara
Canella, Marco
Carmagnola, Lorena
Milazzo, Joëlle
Adreit, Henri
Tharreau, Didier
Poncelet, Nicolas
Vaccino, Patrizia
Valè, Giampiero
author_sort Zampieri, Elisa
collection PubMed
description Rice blast, caused by Pyricularia oryzae, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (Pi) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elite cultivar. In this work, MABC coupled to foreground and background selections based on KASP marker assays has been applied to introgress four Pi genes (Piz, Pib, Pita, and Pik) in a renowned japonica Italian rice variety, highly susceptible to blast. Molecular analyses on the backcross (BC) lines highlighted the presence of an additional blast-resistance gene, the Pita-linked Pita2/Ptr gene, therefore increasing the number of blast-resistance introgressed genes to five. The recurrent genome was recovered up to 95.65%. Several lines carrying four (including Pita2) Pi genes with high recovery percentage levels were also obtained. Phenotypic evaluations confirmed the effectiveness of the pyramided lines against multivirulent strains, which also had broad patterns of resistance in comparison to those expected based on the pyramided Pi genes. The developed blast-resistant japonica lines represent useful donors of multiple blast-resistance genes for future rice-breeding programs related to the japonica group.
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spelling pubmed-99637292023-02-26 Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection Zampieri, Elisa Volante, Andrea Marè, Caterina Orasen, Gabriele Desiderio, Francesca Biselli, Chiara Canella, Marco Carmagnola, Lorena Milazzo, Joëlle Adreit, Henri Tharreau, Didier Poncelet, Nicolas Vaccino, Patrizia Valè, Giampiero Plants (Basel) Article Rice blast, caused by Pyricularia oryzae, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (Pi) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elite cultivar. In this work, MABC coupled to foreground and background selections based on KASP marker assays has been applied to introgress four Pi genes (Piz, Pib, Pita, and Pik) in a renowned japonica Italian rice variety, highly susceptible to blast. Molecular analyses on the backcross (BC) lines highlighted the presence of an additional blast-resistance gene, the Pita-linked Pita2/Ptr gene, therefore increasing the number of blast-resistance introgressed genes to five. The recurrent genome was recovered up to 95.65%. Several lines carrying four (including Pita2) Pi genes with high recovery percentage levels were also obtained. Phenotypic evaluations confirmed the effectiveness of the pyramided lines against multivirulent strains, which also had broad patterns of resistance in comparison to those expected based on the pyramided Pi genes. The developed blast-resistant japonica lines represent useful donors of multiple blast-resistance genes for future rice-breeding programs related to the japonica group. MDPI 2023-02-08 /pmc/articles/PMC9963729/ /pubmed/36840105 http://dx.doi.org/10.3390/plants12040757 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zampieri, Elisa
Volante, Andrea
Marè, Caterina
Orasen, Gabriele
Desiderio, Francesca
Biselli, Chiara
Canella, Marco
Carmagnola, Lorena
Milazzo, Joëlle
Adreit, Henri
Tharreau, Didier
Poncelet, Nicolas
Vaccino, Patrizia
Valè, Giampiero
Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title_full Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title_fullStr Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title_full_unstemmed Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title_short Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection
title_sort marker-assisted pyramiding of blast-resistance genes in a japonica elite rice cultivar through forward and background selection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963729/
https://www.ncbi.nlm.nih.gov/pubmed/36840105
http://dx.doi.org/10.3390/plants12040757
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