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Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest
Salmonella infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant Salmonella infections. Most Salmonella phages to date were collected from human-...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963771/ https://www.ncbi.nlm.nih.gov/pubmed/36835084 http://dx.doi.org/10.3390/ijms24043678 |
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author | Mutusamy, Prasanna Banga Singh, Kirnpal Kaur Su Yin, Lee Petersen, Bent Sicheritz-Ponten, Thomas Clokie, Martha R. J. Loke, Stella Millard, Andrew Parimannan, Sivachandran Rajandas, Heera |
author_facet | Mutusamy, Prasanna Banga Singh, Kirnpal Kaur Su Yin, Lee Petersen, Bent Sicheritz-Ponten, Thomas Clokie, Martha R. J. Loke, Stella Millard, Andrew Parimannan, Sivachandran Rajandas, Heera |
author_sort | Mutusamy, Prasanna |
collection | PubMed |
description | Salmonella infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant Salmonella infections. Most Salmonella phages to date were collected from human-impacted environments. To further explore the Salmonella phage space, and to potentially identify phages with novel characteristics, we characterized Salmonella-specific phages isolated from the Penang National Park, a conserved rainforest. Four phages with a broad lytic spectrum (kills >5 Salmonella serovars) were further characterized; they have isometric heads and cone-shaped tails, and genomes of ~39,900 bp, encoding 49 CDSs. As the genomes share a <95% sequence similarity to known genomes, the phages were classified as a new species within the genus Kayfunavirus. Interestingly, the phages displayed obvious differences in their lytic spectrum and pH stability, despite having a high sequence similarity (~99% ANI). Subsequent analysis revealed that the phages differed in the nucleotide sequence in the tail spike proteins, tail tubular proteins, and portal proteins, suggesting that the SNPs were responsible for their differing phenotypes. Our findings highlight the diversity of novel Salmonella bacteriophages from rainforest regions, which can be explored as an antimicrobial agent against MDR-Salmonella strains. |
format | Online Article Text |
id | pubmed-9963771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99637712023-02-26 Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest Mutusamy, Prasanna Banga Singh, Kirnpal Kaur Su Yin, Lee Petersen, Bent Sicheritz-Ponten, Thomas Clokie, Martha R. J. Loke, Stella Millard, Andrew Parimannan, Sivachandran Rajandas, Heera Int J Mol Sci Article Salmonella infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant Salmonella infections. Most Salmonella phages to date were collected from human-impacted environments. To further explore the Salmonella phage space, and to potentially identify phages with novel characteristics, we characterized Salmonella-specific phages isolated from the Penang National Park, a conserved rainforest. Four phages with a broad lytic spectrum (kills >5 Salmonella serovars) were further characterized; they have isometric heads and cone-shaped tails, and genomes of ~39,900 bp, encoding 49 CDSs. As the genomes share a <95% sequence similarity to known genomes, the phages were classified as a new species within the genus Kayfunavirus. Interestingly, the phages displayed obvious differences in their lytic spectrum and pH stability, despite having a high sequence similarity (~99% ANI). Subsequent analysis revealed that the phages differed in the nucleotide sequence in the tail spike proteins, tail tubular proteins, and portal proteins, suggesting that the SNPs were responsible for their differing phenotypes. Our findings highlight the diversity of novel Salmonella bacteriophages from rainforest regions, which can be explored as an antimicrobial agent against MDR-Salmonella strains. MDPI 2023-02-12 /pmc/articles/PMC9963771/ /pubmed/36835084 http://dx.doi.org/10.3390/ijms24043678 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mutusamy, Prasanna Banga Singh, Kirnpal Kaur Su Yin, Lee Petersen, Bent Sicheritz-Ponten, Thomas Clokie, Martha R. J. Loke, Stella Millard, Andrew Parimannan, Sivachandran Rajandas, Heera Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title | Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title_full | Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title_fullStr | Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title_full_unstemmed | Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title_short | Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest |
title_sort | phenotypic characterization and comparative genomic analysis of novel salmonella bacteriophages isolated from a tropical rainforest |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963771/ https://www.ncbi.nlm.nih.gov/pubmed/36835084 http://dx.doi.org/10.3390/ijms24043678 |
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