Flexible Substrate of Cellulose Fiber/Structured Plasmonic Silver Nanoparticles Applied for Label-Free SERS Detection of Malathion

Surface-enhanced Raman scattering (SERS) is considered an efficient technique providing high sensitivity and fingerprint specificity for the detection of pesticide residues. Recent developments in SERS-based detection aim to create flexible plasmonic substrates that meet the requirements for non-destructive analysis of contaminants on curved surfaces by simply wrapping or wiping. Herein, we reported a flexible SERS substrate based on cellulose fiber (CF) modified with silver nanostructures (AgNS). A silver film was fabricated on the membrane surface with an in situ silver mirror reaction leading to the formation of a AgNS–CF substrate. Then, the substrate was decorated through in situ synthesis of raspberry-like silver nanostructures (rAgNS). The SERS performance of the prepared substrate was tested using 4-mercaptobenzoic acid (4-MBA) as a Raman probe and compared with that of the CF-based plasmonic substrates. The sensitivity of the rAgNS/AgNS–CF substrate was evaluated by determining the detection limit of 4-MBA and an analytical enhancement factor, which were 10 nM and ~10(7), respectively. Further, the proposed flexible rAgNS/AgNS–CF substrate was applied for SERS detection of malathion. The detection limit for malathion reached 0.15 mg/L, which meets the requirements about its maximum residue level in food. Thus, the characteristics of the rAgNS/AgNS–CF substrate demonstrate the potential of its application as a label-free and ready-to-use sensing platform for the SERS detection of trace hazardous substances.