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Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato

Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new che...

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Autores principales: Schiavi, Daniele, Ronchetti, Riccardo, Di Lorenzo, Veronica, Vivani, Riccardo, Giovagnoli, Stefano, Camaioni, Emidio, Balestra, Giorgio M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963933/
https://www.ncbi.nlm.nih.gov/pubmed/36840287
http://dx.doi.org/10.3390/plants12040939
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author Schiavi, Daniele
Ronchetti, Riccardo
Di Lorenzo, Veronica
Vivani, Riccardo
Giovagnoli, Stefano
Camaioni, Emidio
Balestra, Giorgio M.
author_facet Schiavi, Daniele
Ronchetti, Riccardo
Di Lorenzo, Veronica
Vivani, Riccardo
Giovagnoli, Stefano
Camaioni, Emidio
Balestra, Giorgio M.
author_sort Schiavi, Daniele
collection PubMed
description Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new chemical and enzymatic CNC extraction methods from tomato harvest residues were evaluated. The obtained nanomaterials were characterized and tested for their antimicrobial properties on Pseudomonas syringae pv. tomato (Pto), the causal agent of bacterial speck disease on tomato. Both protocols were efficient. The enzymatic extraction method was greener, producing purer CNC at slightly lower yield. The obtained CNC, although they weakly inhibited cell growth and did not promote reactive oxygen species (ROS) formation, provoked bacterial aggregation and the inhibition of biofilm production and swimming motility. Both protocols produced CNC with similar morpho-chemical features, as well as promising antimicrobial activity against plant bacterial pathogens, suggesting their potential role in sustainable crop protection strategies. The new protocols could be a valuable alternative to conventional methods.
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spelling pubmed-99639332023-02-26 Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato Schiavi, Daniele Ronchetti, Riccardo Di Lorenzo, Veronica Vivani, Riccardo Giovagnoli, Stefano Camaioni, Emidio Balestra, Giorgio M. Plants (Basel) Article Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new chemical and enzymatic CNC extraction methods from tomato harvest residues were evaluated. The obtained nanomaterials were characterized and tested for their antimicrobial properties on Pseudomonas syringae pv. tomato (Pto), the causal agent of bacterial speck disease on tomato. Both protocols were efficient. The enzymatic extraction method was greener, producing purer CNC at slightly lower yield. The obtained CNC, although they weakly inhibited cell growth and did not promote reactive oxygen species (ROS) formation, provoked bacterial aggregation and the inhibition of biofilm production and swimming motility. Both protocols produced CNC with similar morpho-chemical features, as well as promising antimicrobial activity against plant bacterial pathogens, suggesting their potential role in sustainable crop protection strategies. The new protocols could be a valuable alternative to conventional methods. MDPI 2023-02-18 /pmc/articles/PMC9963933/ /pubmed/36840287 http://dx.doi.org/10.3390/plants12040939 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schiavi, Daniele
Ronchetti, Riccardo
Di Lorenzo, Veronica
Vivani, Riccardo
Giovagnoli, Stefano
Camaioni, Emidio
Balestra, Giorgio M.
Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title_full Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title_fullStr Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title_full_unstemmed Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title_short Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato
title_sort sustainable protocols for cellulose nanocrystals synthesis from tomato waste and their antimicrobial properties against pseudomonas syringae pv. tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963933/
https://www.ncbi.nlm.nih.gov/pubmed/36840287
http://dx.doi.org/10.3390/plants12040939
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