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LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats

Parsley (Petroselinum sativum Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To exte...

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Autores principales: Slighoua, Meryem, Mahdi, Ismail, Moussaid, Fatima Zahrae, Kamaly, Omkulthom Al, Amrati, Fatima Ez-zahra, Conte, Raffaele, Drioiche, Aziz, Saleh, Asmaa, Housseini, Abdelilah Iraqi, Bari, Amina, Bousta, Dalila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963972/
https://www.ncbi.nlm.nih.gov/pubmed/36837879
http://dx.doi.org/10.3390/metabo13020260
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author Slighoua, Meryem
Mahdi, Ismail
Moussaid, Fatima Zahrae
Kamaly, Omkulthom Al
Amrati, Fatima Ez-zahra
Conte, Raffaele
Drioiche, Aziz
Saleh, Asmaa
Housseini, Abdelilah Iraqi
Bari, Amina
Bousta, Dalila
author_facet Slighoua, Meryem
Mahdi, Ismail
Moussaid, Fatima Zahrae
Kamaly, Omkulthom Al
Amrati, Fatima Ez-zahra
Conte, Raffaele
Drioiche, Aziz
Saleh, Asmaa
Housseini, Abdelilah Iraqi
Bari, Amina
Bousta, Dalila
author_sort Slighoua, Meryem
collection PubMed
description Parsley (Petroselinum sativum Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To extend our investigation, we here characterized the phytochemical composition of the hydro-ethanolic extract using LC-MS/MS and GC-MS upon silylation, and evaluated the antioxidant, analgesic, antimicrobial, and wound healing activities of its hydro-ethanolic and polyphenolic fraction. The antioxidant property was assessed using FRAP, DPPH, and TAC assays. The antimicrobial activity was tested against four wound infectious microbes (Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans). The analgesic effect was studied using acetic acid (counting the number of writhes) and formalin (recording the licking and biting times) injections while the wound healing activity was evaluated using burn model in vivo. The LC-MS/MS showed that the hydro-ethanolic contains four polyphenols (oleuropein, arbutin, myricetin, and naringin) while GC-MS revealed that it contains 20 compounds including malic acid, D-glucose, and galactofuranoside. The hydro-ethanolic (1000 mg/kg) decreased abdominal writhes (38.96%) and licking time (37.34%). It also elicited a strong antioxidant activity using DPPH method (IC(50) = 19.38 ± 0.15 µg/mL). Polyphenols exhibited a good antimicrobial effect (MIC = 3.125–12.5 mg/mL). Moreover, both extracts showed high wound contraction by 97.17% and 94.98%, respectively. This study provides evidence that P. sativum could serve as a source of bio-compounds exhibiting analgesic effect and their promising application in mitigating ROS-related disorders, impeding wound infections, and enhancing burn healing.
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spelling pubmed-99639722023-02-26 LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats Slighoua, Meryem Mahdi, Ismail Moussaid, Fatima Zahrae Kamaly, Omkulthom Al Amrati, Fatima Ez-zahra Conte, Raffaele Drioiche, Aziz Saleh, Asmaa Housseini, Abdelilah Iraqi Bari, Amina Bousta, Dalila Metabolites Article Parsley (Petroselinum sativum Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To extend our investigation, we here characterized the phytochemical composition of the hydro-ethanolic extract using LC-MS/MS and GC-MS upon silylation, and evaluated the antioxidant, analgesic, antimicrobial, and wound healing activities of its hydro-ethanolic and polyphenolic fraction. The antioxidant property was assessed using FRAP, DPPH, and TAC assays. The antimicrobial activity was tested against four wound infectious microbes (Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans). The analgesic effect was studied using acetic acid (counting the number of writhes) and formalin (recording the licking and biting times) injections while the wound healing activity was evaluated using burn model in vivo. The LC-MS/MS showed that the hydro-ethanolic contains four polyphenols (oleuropein, arbutin, myricetin, and naringin) while GC-MS revealed that it contains 20 compounds including malic acid, D-glucose, and galactofuranoside. The hydro-ethanolic (1000 mg/kg) decreased abdominal writhes (38.96%) and licking time (37.34%). It also elicited a strong antioxidant activity using DPPH method (IC(50) = 19.38 ± 0.15 µg/mL). Polyphenols exhibited a good antimicrobial effect (MIC = 3.125–12.5 mg/mL). Moreover, both extracts showed high wound contraction by 97.17% and 94.98%, respectively. This study provides evidence that P. sativum could serve as a source of bio-compounds exhibiting analgesic effect and their promising application in mitigating ROS-related disorders, impeding wound infections, and enhancing burn healing. MDPI 2023-02-11 /pmc/articles/PMC9963972/ /pubmed/36837879 http://dx.doi.org/10.3390/metabo13020260 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Slighoua, Meryem
Mahdi, Ismail
Moussaid, Fatima Zahrae
Kamaly, Omkulthom Al
Amrati, Fatima Ez-zahra
Conte, Raffaele
Drioiche, Aziz
Saleh, Asmaa
Housseini, Abdelilah Iraqi
Bari, Amina
Bousta, Dalila
LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title_full LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title_fullStr LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title_full_unstemmed LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title_short LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats
title_sort lc-ms/ms and gc/ms profiling of petroselinum sativum hoffm. and its topical application on burn wound healing and related analgesic potential in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963972/
https://www.ncbi.nlm.nih.gov/pubmed/36837879
http://dx.doi.org/10.3390/metabo13020260
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