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Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation
Titanium dioxide nanoparticles (TiO(2) NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO(2) NPs using a whole-body inhalation chamber model are rare. In this...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964099/ https://www.ncbi.nlm.nih.gov/pubmed/36839135 http://dx.doi.org/10.3390/nano13040767 |
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author | Lehotska Mikusova, Miroslava Busova, Milena Tulinska, Jana Masanova, Vlasta Liskova, Aurelia Uhnakova, Iveta Dusinska, Maria Krivosikova, Zora Rollerova, Eva Alacova, Radka Wsolova, Ladislava Horvathova, Mira Szabova, Michaela Lukan, Norbert Vecera, Zbynek Coufalik, Pavel Krumal, Kamil Alexa, Lukas Thon, Vojtech Piler, Pavel Buchtova, Marcela Vrlikova, Lucie Moravec, Pavel Galanda, Dusan Mikuska, Pavel |
author_facet | Lehotska Mikusova, Miroslava Busova, Milena Tulinska, Jana Masanova, Vlasta Liskova, Aurelia Uhnakova, Iveta Dusinska, Maria Krivosikova, Zora Rollerova, Eva Alacova, Radka Wsolova, Ladislava Horvathova, Mira Szabova, Michaela Lukan, Norbert Vecera, Zbynek Coufalik, Pavel Krumal, Kamil Alexa, Lukas Thon, Vojtech Piler, Pavel Buchtova, Marcela Vrlikova, Lucie Moravec, Pavel Galanda, Dusan Mikuska, Pavel |
author_sort | Lehotska Mikusova, Miroslava |
collection | PubMed |
description | Titanium dioxide nanoparticles (TiO(2) NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO(2) NPs using a whole-body inhalation chamber model are rare. In this study, the profile of lymphocyte markers, functional immunoassays, and antioxidant defense markers were analyzed to evaluate the potential adverse effects of seven-week inhalation exposure to two different concentrations of TiO(2) NPs (0.00167 and 0.1308 mg TiO(2)/m(3)) in mice. A dose-dependent effect of TiO(2) NPs on innate immunity was evident in the form of stimulated phagocytic activity of monocytes in low-dose mice and suppressed secretory function of monocytes (IL-18) in high-dose animals. The effect of TiO(2) NPs on adaptive immunity, manifested in the spleen by a decrease in the percentage of T-cells, a reduction in T-helper cells, and a dose-dependent decrease in lymphocyte cytokine production, may indicate immunosuppression in exposed mice. The dose-dependent increase in GSH concentration and GSH/GSSG ratio in whole blood demonstrated stimulated antioxidant defense against oxidative stress induced by TiO(2) NP exposure. |
format | Online Article Text |
id | pubmed-9964099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99640992023-02-26 Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation Lehotska Mikusova, Miroslava Busova, Milena Tulinska, Jana Masanova, Vlasta Liskova, Aurelia Uhnakova, Iveta Dusinska, Maria Krivosikova, Zora Rollerova, Eva Alacova, Radka Wsolova, Ladislava Horvathova, Mira Szabova, Michaela Lukan, Norbert Vecera, Zbynek Coufalik, Pavel Krumal, Kamil Alexa, Lukas Thon, Vojtech Piler, Pavel Buchtova, Marcela Vrlikova, Lucie Moravec, Pavel Galanda, Dusan Mikuska, Pavel Nanomaterials (Basel) Article Titanium dioxide nanoparticles (TiO(2) NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO(2) NPs using a whole-body inhalation chamber model are rare. In this study, the profile of lymphocyte markers, functional immunoassays, and antioxidant defense markers were analyzed to evaluate the potential adverse effects of seven-week inhalation exposure to two different concentrations of TiO(2) NPs (0.00167 and 0.1308 mg TiO(2)/m(3)) in mice. A dose-dependent effect of TiO(2) NPs on innate immunity was evident in the form of stimulated phagocytic activity of monocytes in low-dose mice and suppressed secretory function of monocytes (IL-18) in high-dose animals. The effect of TiO(2) NPs on adaptive immunity, manifested in the spleen by a decrease in the percentage of T-cells, a reduction in T-helper cells, and a dose-dependent decrease in lymphocyte cytokine production, may indicate immunosuppression in exposed mice. The dose-dependent increase in GSH concentration and GSH/GSSG ratio in whole blood demonstrated stimulated antioxidant defense against oxidative stress induced by TiO(2) NP exposure. MDPI 2023-02-18 /pmc/articles/PMC9964099/ /pubmed/36839135 http://dx.doi.org/10.3390/nano13040767 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lehotska Mikusova, Miroslava Busova, Milena Tulinska, Jana Masanova, Vlasta Liskova, Aurelia Uhnakova, Iveta Dusinska, Maria Krivosikova, Zora Rollerova, Eva Alacova, Radka Wsolova, Ladislava Horvathova, Mira Szabova, Michaela Lukan, Norbert Vecera, Zbynek Coufalik, Pavel Krumal, Kamil Alexa, Lukas Thon, Vojtech Piler, Pavel Buchtova, Marcela Vrlikova, Lucie Moravec, Pavel Galanda, Dusan Mikuska, Pavel Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title | Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title_full | Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title_fullStr | Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title_full_unstemmed | Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title_short | Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation |
title_sort | titanium dioxide nanoparticles modulate systemic immune response and increase levels of reduced glutathione in mice after seven-week inhalation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964099/ https://www.ncbi.nlm.nih.gov/pubmed/36839135 http://dx.doi.org/10.3390/nano13040767 |
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