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The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study

Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), s...

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Autores principales: d’Alessandro, Miriana, Gangi, Sara, Soccio, Piera, Cantó, Elisabet, Osuna-Gómez, Rubén, Bergantini, Laura, Cameli, Paolo, Fabbri, Gaia, Croce, Sara, Scioscia, Giulia, Montuori, Giusy, Fanetti, Matteo, Moriondo, Giorgia, Mezzasalma, Fabrizio, Castillo, Diego, Lacedonia, Donato, Vidal, Silvia, Bargagli, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964169/
https://www.ncbi.nlm.nih.gov/pubmed/36835481
http://dx.doi.org/10.3390/ijms24044071
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author d’Alessandro, Miriana
Gangi, Sara
Soccio, Piera
Cantó, Elisabet
Osuna-Gómez, Rubén
Bergantini, Laura
Cameli, Paolo
Fabbri, Gaia
Croce, Sara
Scioscia, Giulia
Montuori, Giusy
Fanetti, Matteo
Moriondo, Giorgia
Mezzasalma, Fabrizio
Castillo, Diego
Lacedonia, Donato
Vidal, Silvia
Bargagli, Elena
author_facet d’Alessandro, Miriana
Gangi, Sara
Soccio, Piera
Cantó, Elisabet
Osuna-Gómez, Rubén
Bergantini, Laura
Cameli, Paolo
Fabbri, Gaia
Croce, Sara
Scioscia, Giulia
Montuori, Giusy
Fanetti, Matteo
Moriondo, Giorgia
Mezzasalma, Fabrizio
Castillo, Diego
Lacedonia, Donato
Vidal, Silvia
Bargagli, Elena
author_sort d’Alessandro, Miriana
collection PubMed
description Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis and hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona and Foggia University Hospitals were enrolled. BAL supernatants were used to isolate the EVs. They were characterized by flow cytometry assay through MACSPlex Exsome KIT. The majority of alveolar EV markers were related to the fibrotic damage. CD56, CD105, CD142, CD31 and CD49e were exclusively expressed by alveolar samples from IPF patients, while HP showed only CD86 and CD24. Some EV markers were common between HP and sarcoidosis (CD11c, CD1c, CD209, CD4, CD40, CD44, CD8). Principal component analysis distinguished the three groups based on EV markers with total variance of 60.08%. This study has demonstrated the validity of the flow cytometric method to phenotype and characterize EV surface markers in BAL samples. The two granulomatous diseases, sarcoidosis and HP, cohorts shared alveolar EV markers not revealed in IPF patients. Our findings demonstrated the viability of the alveolar compartment allowing identification of lung-specific markers for IPF and HP.
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spelling pubmed-99641692023-02-26 The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study d’Alessandro, Miriana Gangi, Sara Soccio, Piera Cantó, Elisabet Osuna-Gómez, Rubén Bergantini, Laura Cameli, Paolo Fabbri, Gaia Croce, Sara Scioscia, Giulia Montuori, Giusy Fanetti, Matteo Moriondo, Giorgia Mezzasalma, Fabrizio Castillo, Diego Lacedonia, Donato Vidal, Silvia Bargagli, Elena Int J Mol Sci Article Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis and hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona and Foggia University Hospitals were enrolled. BAL supernatants were used to isolate the EVs. They were characterized by flow cytometry assay through MACSPlex Exsome KIT. The majority of alveolar EV markers were related to the fibrotic damage. CD56, CD105, CD142, CD31 and CD49e were exclusively expressed by alveolar samples from IPF patients, while HP showed only CD86 and CD24. Some EV markers were common between HP and sarcoidosis (CD11c, CD1c, CD209, CD4, CD40, CD44, CD8). Principal component analysis distinguished the three groups based on EV markers with total variance of 60.08%. This study has demonstrated the validity of the flow cytometric method to phenotype and characterize EV surface markers in BAL samples. The two granulomatous diseases, sarcoidosis and HP, cohorts shared alveolar EV markers not revealed in IPF patients. Our findings demonstrated the viability of the alveolar compartment allowing identification of lung-specific markers for IPF and HP. MDPI 2023-02-17 /pmc/articles/PMC9964169/ /pubmed/36835481 http://dx.doi.org/10.3390/ijms24044071 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
d’Alessandro, Miriana
Gangi, Sara
Soccio, Piera
Cantó, Elisabet
Osuna-Gómez, Rubén
Bergantini, Laura
Cameli, Paolo
Fabbri, Gaia
Croce, Sara
Scioscia, Giulia
Montuori, Giusy
Fanetti, Matteo
Moriondo, Giorgia
Mezzasalma, Fabrizio
Castillo, Diego
Lacedonia, Donato
Vidal, Silvia
Bargagli, Elena
The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title_full The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title_fullStr The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title_full_unstemmed The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title_short The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study
title_sort effects of interstitial lung diseases on alveolar extracellular vesicles profile: a multicenter study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964169/
https://www.ncbi.nlm.nih.gov/pubmed/36835481
http://dx.doi.org/10.3390/ijms24044071
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