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Improved DNA Vaccine Delivery with Needle-Free Injection Systems
DNA vaccines have inherent advantages compared to other vaccine types, including safety, rapid design and construction, ease and speed to manufacture, and thermostability. However, a major drawback of candidate DNA vaccines delivered by needle and syringe is the poor immunogenicity associated with i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964240/ https://www.ncbi.nlm.nih.gov/pubmed/36851159 http://dx.doi.org/10.3390/vaccines11020280 |
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author | Ledesma-Feliciano, Carmen Chapman, Ros Hooper, Jay W. Elma, Kira Zehrung, Darin Brennan, Miles B. Spiegel, Erin K. |
author_facet | Ledesma-Feliciano, Carmen Chapman, Ros Hooper, Jay W. Elma, Kira Zehrung, Darin Brennan, Miles B. Spiegel, Erin K. |
author_sort | Ledesma-Feliciano, Carmen |
collection | PubMed |
description | DNA vaccines have inherent advantages compared to other vaccine types, including safety, rapid design and construction, ease and speed to manufacture, and thermostability. However, a major drawback of candidate DNA vaccines delivered by needle and syringe is the poor immunogenicity associated with inefficient cellular uptake of the DNA. This uptake is essential because the target vaccine antigen is produced within cells and then presented to the immune system. Multiple techniques have been employed to boost the immunogenicity and protective efficacy of DNA vaccines, including physical delivery methods, molecular and traditional adjuvants, and genetic sequence enhancements. Needle-free injection systems (NFIS) are an attractive alternative due to the induction of potent immunogenicity, enhanced protective efficacy, and elimination of needles. These advantages led to a milestone achievement in the field with the approval for Restricted Use in Emergency Situation of a DNA vaccine against COVID-19, delivered exclusively with NFIS. In this review, we discuss physical delivery methods for DNA vaccines with an emphasis on commercially available NFIS and their resulting safety, immunogenic effectiveness, and protective efficacy. As is discussed, prophylactic DNA vaccines delivered by NFIS tend to induce non-inferior immunogenicity to electroporation and enhanced responses compared to needle and syringe. |
format | Online Article Text |
id | pubmed-9964240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99642402023-02-26 Improved DNA Vaccine Delivery with Needle-Free Injection Systems Ledesma-Feliciano, Carmen Chapman, Ros Hooper, Jay W. Elma, Kira Zehrung, Darin Brennan, Miles B. Spiegel, Erin K. Vaccines (Basel) Review DNA vaccines have inherent advantages compared to other vaccine types, including safety, rapid design and construction, ease and speed to manufacture, and thermostability. However, a major drawback of candidate DNA vaccines delivered by needle and syringe is the poor immunogenicity associated with inefficient cellular uptake of the DNA. This uptake is essential because the target vaccine antigen is produced within cells and then presented to the immune system. Multiple techniques have been employed to boost the immunogenicity and protective efficacy of DNA vaccines, including physical delivery methods, molecular and traditional adjuvants, and genetic sequence enhancements. Needle-free injection systems (NFIS) are an attractive alternative due to the induction of potent immunogenicity, enhanced protective efficacy, and elimination of needles. These advantages led to a milestone achievement in the field with the approval for Restricted Use in Emergency Situation of a DNA vaccine against COVID-19, delivered exclusively with NFIS. In this review, we discuss physical delivery methods for DNA vaccines with an emphasis on commercially available NFIS and their resulting safety, immunogenic effectiveness, and protective efficacy. As is discussed, prophylactic DNA vaccines delivered by NFIS tend to induce non-inferior immunogenicity to electroporation and enhanced responses compared to needle and syringe. MDPI 2023-01-28 /pmc/articles/PMC9964240/ /pubmed/36851159 http://dx.doi.org/10.3390/vaccines11020280 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Ledesma-Feliciano, Carmen Chapman, Ros Hooper, Jay W. Elma, Kira Zehrung, Darin Brennan, Miles B. Spiegel, Erin K. Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title | Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title_full | Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title_fullStr | Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title_full_unstemmed | Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title_short | Improved DNA Vaccine Delivery with Needle-Free Injection Systems |
title_sort | improved dna vaccine delivery with needle-free injection systems |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964240/ https://www.ncbi.nlm.nih.gov/pubmed/36851159 http://dx.doi.org/10.3390/vaccines11020280 |
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