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Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells

Lavandula pedunculata (Mill.) Cav., Mentha cervina L. and Thymus mastichina (L.) L. subsp. mastichina are representative species of the Côa Valley’s flora, a Portuguese UNESCO World Heritage Site. L. pedunculata and T. mastichina are traditionally used to preserve olives and to aromatize bonfires on...

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Autores principales: Marques, Mário Pedro, Neves, Beatriz Guapo, Varela, Carla, Zuzarte, Mónica, Gonçalves, Ana Cristina, Dias, Maria Inês, Amaral, Joana S., Barros, Lillian, Magalhães, Mariana, Cabral, Célia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964318/
https://www.ncbi.nlm.nih.gov/pubmed/36839664
http://dx.doi.org/10.3390/pharmaceutics15020341
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author Marques, Mário Pedro
Neves, Beatriz Guapo
Varela, Carla
Zuzarte, Mónica
Gonçalves, Ana Cristina
Dias, Maria Inês
Amaral, Joana S.
Barros, Lillian
Magalhães, Mariana
Cabral, Célia
author_facet Marques, Mário Pedro
Neves, Beatriz Guapo
Varela, Carla
Zuzarte, Mónica
Gonçalves, Ana Cristina
Dias, Maria Inês
Amaral, Joana S.
Barros, Lillian
Magalhães, Mariana
Cabral, Célia
author_sort Marques, Mário Pedro
collection PubMed
description Lavandula pedunculata (Mill.) Cav., Mentha cervina L. and Thymus mastichina (L.) L. subsp. mastichina are representative species of the Côa Valley’s flora, a Portuguese UNESCO World Heritage Site. L. pedunculata and T. mastichina are traditionally used to preserve olives and to aromatize bonfires on Saint John’s Eve, while M. cervina is mainly used as a spice for river fish dishes. Despite their traditional uses, these aromatic plants are still undervalued, and literature regarding their bioactivity, especially anticancer, is scarce. In this work, the morphology of secretory structures was assessed by scanning electron microscopy (SEM), and the composition of essential oils (EOs) was characterized by gas chromatography-mass spectrometry (GC-MS). The study proceeded with cytotoxic evaluation of EOs in tumor and non-tumor cells with the cell death mechanism explored in glioblastoma (GB) cells. L. pedunculata EO presented the most pronounced cytotoxic/antiproliferative activity against tumor cells, with moderate cytotoxicity against non-tumor cells. Whereas, M. cervina EO exhibited a slightly lower cytotoxic effect against tumor cells and did not affect the viability of non-tumor cells. Meanwhile, T. mastichina EO did not induce a strong cytotoxic effect against GB cells. L. pedunculata and M. cervina EOs lead to cell death by inducing apoptosis in a dose-dependent manner. The present study suggests that L. pedunculata and M. cervina EOs have a strong cytotoxic and antiproliferative potential to be further studied as efficient antitumor agents.
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spelling pubmed-99643182023-02-26 Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells Marques, Mário Pedro Neves, Beatriz Guapo Varela, Carla Zuzarte, Mónica Gonçalves, Ana Cristina Dias, Maria Inês Amaral, Joana S. Barros, Lillian Magalhães, Mariana Cabral, Célia Pharmaceutics Article Lavandula pedunculata (Mill.) Cav., Mentha cervina L. and Thymus mastichina (L.) L. subsp. mastichina are representative species of the Côa Valley’s flora, a Portuguese UNESCO World Heritage Site. L. pedunculata and T. mastichina are traditionally used to preserve olives and to aromatize bonfires on Saint John’s Eve, while M. cervina is mainly used as a spice for river fish dishes. Despite their traditional uses, these aromatic plants are still undervalued, and literature regarding their bioactivity, especially anticancer, is scarce. In this work, the morphology of secretory structures was assessed by scanning electron microscopy (SEM), and the composition of essential oils (EOs) was characterized by gas chromatography-mass spectrometry (GC-MS). The study proceeded with cytotoxic evaluation of EOs in tumor and non-tumor cells with the cell death mechanism explored in glioblastoma (GB) cells. L. pedunculata EO presented the most pronounced cytotoxic/antiproliferative activity against tumor cells, with moderate cytotoxicity against non-tumor cells. Whereas, M. cervina EO exhibited a slightly lower cytotoxic effect against tumor cells and did not affect the viability of non-tumor cells. Meanwhile, T. mastichina EO did not induce a strong cytotoxic effect against GB cells. L. pedunculata and M. cervina EOs lead to cell death by inducing apoptosis in a dose-dependent manner. The present study suggests that L. pedunculata and M. cervina EOs have a strong cytotoxic and antiproliferative potential to be further studied as efficient antitumor agents. MDPI 2023-01-19 /pmc/articles/PMC9964318/ /pubmed/36839664 http://dx.doi.org/10.3390/pharmaceutics15020341 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marques, Mário Pedro
Neves, Beatriz Guapo
Varela, Carla
Zuzarte, Mónica
Gonçalves, Ana Cristina
Dias, Maria Inês
Amaral, Joana S.
Barros, Lillian
Magalhães, Mariana
Cabral, Célia
Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title_full Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title_fullStr Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title_full_unstemmed Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title_short Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells
title_sort essential oils from côa valley lamiaceae species: cytotoxicity and antiproliferative effect on glioblastoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964318/
https://www.ncbi.nlm.nih.gov/pubmed/36839664
http://dx.doi.org/10.3390/pharmaceutics15020341
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