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Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro

Probiotic bacteria have many protective effects against inflammatory disorders, though the mechanisms underlying their actions are poorly understood. The Lab4b consortium of probiotics contains four strains of lactic acid bacteria and bifidobacteria that are reflective of the gut of newborn babies a...

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Autores principales: O’Morain, Victoria L., Chen, Jing, Plummer, Sue F., Michael, Daryn R., Ramji, Dipak P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964490/
https://www.ncbi.nlm.nih.gov/pubmed/36835055
http://dx.doi.org/10.3390/ijms24043639
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author O’Morain, Victoria L.
Chen, Jing
Plummer, Sue F.
Michael, Daryn R.
Ramji, Dipak P.
author_facet O’Morain, Victoria L.
Chen, Jing
Plummer, Sue F.
Michael, Daryn R.
Ramji, Dipak P.
author_sort O’Morain, Victoria L.
collection PubMed
description Probiotic bacteria have many protective effects against inflammatory disorders, though the mechanisms underlying their actions are poorly understood. The Lab4b consortium of probiotics contains four strains of lactic acid bacteria and bifidobacteria that are reflective of the gut of newborn babies and infants. The effect of Lab4b on atherosclerosis, an inflammatory disorder of the vasculature, has not yet been determined and was investigated on key processes associated with this disease in human monocytes/macrophages and vascular smooth muscle cells in vitro. The Lab4b conditioned medium (CM) attenuated chemokine-driven monocytic migration, monocyte/macrophage proliferation, uptake of modified LDL and macropinocytosis in macrophages together with the proliferation and platelet-derived growth factor-induced migration of vascular smooth muscle cells. The Lab4b CM also induced phagocytosis in macrophages and cholesterol efflux from macrophage-derived foam cells. The effect of Lab4b CM on macrophage foam cell formation was associated with a decrease in the expression of several key genes implicated in the uptake of modified LDL and induced expression of those involved in cholesterol efflux. These studies reveal, for the first time, several anti-atherogenic actions of Lab4b and strongly implicate further studies in mouse models of the disease in vivo and in clinical trials.
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spelling pubmed-99644902023-02-26 Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro O’Morain, Victoria L. Chen, Jing Plummer, Sue F. Michael, Daryn R. Ramji, Dipak P. Int J Mol Sci Article Probiotic bacteria have many protective effects against inflammatory disorders, though the mechanisms underlying their actions are poorly understood. The Lab4b consortium of probiotics contains four strains of lactic acid bacteria and bifidobacteria that are reflective of the gut of newborn babies and infants. The effect of Lab4b on atherosclerosis, an inflammatory disorder of the vasculature, has not yet been determined and was investigated on key processes associated with this disease in human monocytes/macrophages and vascular smooth muscle cells in vitro. The Lab4b conditioned medium (CM) attenuated chemokine-driven monocytic migration, monocyte/macrophage proliferation, uptake of modified LDL and macropinocytosis in macrophages together with the proliferation and platelet-derived growth factor-induced migration of vascular smooth muscle cells. The Lab4b CM also induced phagocytosis in macrophages and cholesterol efflux from macrophage-derived foam cells. The effect of Lab4b CM on macrophage foam cell formation was associated with a decrease in the expression of several key genes implicated in the uptake of modified LDL and induced expression of those involved in cholesterol efflux. These studies reveal, for the first time, several anti-atherogenic actions of Lab4b and strongly implicate further studies in mouse models of the disease in vivo and in clinical trials. MDPI 2023-02-11 /pmc/articles/PMC9964490/ /pubmed/36835055 http://dx.doi.org/10.3390/ijms24043639 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O’Morain, Victoria L.
Chen, Jing
Plummer, Sue F.
Michael, Daryn R.
Ramji, Dipak P.
Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title_full Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title_fullStr Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title_full_unstemmed Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title_short Anti-Atherogenic Actions of the Lab4b Consortium of Probiotics In Vitro
title_sort anti-atherogenic actions of the lab4b consortium of probiotics in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964490/
https://www.ncbi.nlm.nih.gov/pubmed/36835055
http://dx.doi.org/10.3390/ijms24043639
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