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Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines
More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as pote...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964626/ https://www.ncbi.nlm.nih.gov/pubmed/36835538 http://dx.doi.org/10.3390/ijms24044122 |
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author | Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben |
author_facet | Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben |
author_sort | Soares, Sílvia |
collection | PubMed |
description | More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNP(sp)-PEG, star, AuNP(st)-PEG, and rods, AuNP(r)-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells. |
format | Online Article Text |
id | pubmed-9964626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99646262023-02-26 Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben Int J Mol Sci Article More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNP(sp)-PEG, star, AuNP(st)-PEG, and rods, AuNP(r)-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells. MDPI 2023-02-18 /pmc/articles/PMC9964626/ /pubmed/36835538 http://dx.doi.org/10.3390/ijms24044122 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title | Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title_full | Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title_fullStr | Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title_full_unstemmed | Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title_short | Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell Lines |
title_sort | application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964626/ https://www.ncbi.nlm.nih.gov/pubmed/36835538 http://dx.doi.org/10.3390/ijms24044122 |
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