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Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults

Triacylglycerols (TAGs) and cholesterol lipoprotein levels are widely used to predict cardiovascular risk and metabolic disorders. The aim of this study is to determine how the comprehensive lipidome (individual molecular lipid species) determined by mass spectrometry is correlated to the serum whol...

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Autores principales: Sánchez-Vinces, Salvador, Garcia, Pedro Henrique Dias, Silva, Alex Ap. Rosini, Fernandes, Anna Maria Alves de Piloto, Barreto, Joyce Aparecida, Duarte, Gustavo Henrique Bueno, Antonio, Marcia Aparecida, Birbrair, Alexander, Porcari, Andreia M., Carvalho, Patricia de Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964724/
https://www.ncbi.nlm.nih.gov/pubmed/36837840
http://dx.doi.org/10.3390/metabo13020222
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author Sánchez-Vinces, Salvador
Garcia, Pedro Henrique Dias
Silva, Alex Ap. Rosini
Fernandes, Anna Maria Alves de Piloto
Barreto, Joyce Aparecida
Duarte, Gustavo Henrique Bueno
Antonio, Marcia Aparecida
Birbrair, Alexander
Porcari, Andreia M.
Carvalho, Patricia de Oliveira
author_facet Sánchez-Vinces, Salvador
Garcia, Pedro Henrique Dias
Silva, Alex Ap. Rosini
Fernandes, Anna Maria Alves de Piloto
Barreto, Joyce Aparecida
Duarte, Gustavo Henrique Bueno
Antonio, Marcia Aparecida
Birbrair, Alexander
Porcari, Andreia M.
Carvalho, Patricia de Oliveira
author_sort Sánchez-Vinces, Salvador
collection PubMed
description Triacylglycerols (TAGs) and cholesterol lipoprotein levels are widely used to predict cardiovascular risk and metabolic disorders. The aim of this study is to determine how the comprehensive lipidome (individual molecular lipid species) determined by mass spectrometry is correlated to the serum whole-lipidic profile of adults with different lipidemic conditions. The study included samples from 128 adults of both sexes, and they were separated into four groups according to their lipid profile: Group I—normolipidemic (TAG < 150 mg/dL, LDL-C < 160 mg/dL and HDL-c > 40 mg/dL); Group II—isolated hypertriglyceridemia (TAG ≥ 150 mg/dL); Group III—isolated hypercholesterolemia (LDL-C ≥ 160 mg/dL) and Group IV—mixed dyslipidemia. An untargeted mass spectrometry (MS)-based approach was applied to determine the lipidomic signature of 32 healthy and 96 dyslipidemic adults. Limma linear regression was used to predict the correlation of serum TAGs and cholesterol lipoprotein levels with the abundance of the identified MS-annotated lipids found in the subgroups of subjects. Serum TAG levels of dyslipidemic adults have a positive correlation with some of the MS-annotated specific TAGs and ceramides (Cer) and a negative correlation with sphingomyelins (SMs). High-density lipoprotein-cholesterol (HDL-C) levels are positively correlated with some groups of glycerophosphocholine, while low-density lipoprotein-cholesterol (LDL-C) has a positive correlation with SMs.
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spelling pubmed-99647242023-02-26 Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults Sánchez-Vinces, Salvador Garcia, Pedro Henrique Dias Silva, Alex Ap. Rosini Fernandes, Anna Maria Alves de Piloto Barreto, Joyce Aparecida Duarte, Gustavo Henrique Bueno Antonio, Marcia Aparecida Birbrair, Alexander Porcari, Andreia M. Carvalho, Patricia de Oliveira Metabolites Article Triacylglycerols (TAGs) and cholesterol lipoprotein levels are widely used to predict cardiovascular risk and metabolic disorders. The aim of this study is to determine how the comprehensive lipidome (individual molecular lipid species) determined by mass spectrometry is correlated to the serum whole-lipidic profile of adults with different lipidemic conditions. The study included samples from 128 adults of both sexes, and they were separated into four groups according to their lipid profile: Group I—normolipidemic (TAG < 150 mg/dL, LDL-C < 160 mg/dL and HDL-c > 40 mg/dL); Group II—isolated hypertriglyceridemia (TAG ≥ 150 mg/dL); Group III—isolated hypercholesterolemia (LDL-C ≥ 160 mg/dL) and Group IV—mixed dyslipidemia. An untargeted mass spectrometry (MS)-based approach was applied to determine the lipidomic signature of 32 healthy and 96 dyslipidemic adults. Limma linear regression was used to predict the correlation of serum TAGs and cholesterol lipoprotein levels with the abundance of the identified MS-annotated lipids found in the subgroups of subjects. Serum TAG levels of dyslipidemic adults have a positive correlation with some of the MS-annotated specific TAGs and ceramides (Cer) and a negative correlation with sphingomyelins (SMs). High-density lipoprotein-cholesterol (HDL-C) levels are positively correlated with some groups of glycerophosphocholine, while low-density lipoprotein-cholesterol (LDL-C) has a positive correlation with SMs. MDPI 2023-02-03 /pmc/articles/PMC9964724/ /pubmed/36837840 http://dx.doi.org/10.3390/metabo13020222 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Vinces, Salvador
Garcia, Pedro Henrique Dias
Silva, Alex Ap. Rosini
Fernandes, Anna Maria Alves de Piloto
Barreto, Joyce Aparecida
Duarte, Gustavo Henrique Bueno
Antonio, Marcia Aparecida
Birbrair, Alexander
Porcari, Andreia M.
Carvalho, Patricia de Oliveira
Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title_full Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title_fullStr Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title_full_unstemmed Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title_short Mass-Spectrometry-Based Lipidomics Discriminates Specific Changes in Lipid Classes in Healthy and Dyslipidemic Adults
title_sort mass-spectrometry-based lipidomics discriminates specific changes in lipid classes in healthy and dyslipidemic adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964724/
https://www.ncbi.nlm.nih.gov/pubmed/36837840
http://dx.doi.org/10.3390/metabo13020222
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