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Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types

Biopolymer-like collagen has great industrial potential in terms of its excellent properties, such as strong biocompatibility, high degradability, and low antigenicity. Collagen derived from fish by-products is preferable as it is safer (free from transmittable diseases) and acceptable to most relig...

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Autores principales: Fatiroi, Nurul Syazwanie, Jaziri, Abdul Aziz, Shapawi, Rossita, Mokhtar, Ruzaidi Azli Mohd, Noordin, Wan Norhana Md., Huda, Nurul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964761/
https://www.ncbi.nlm.nih.gov/pubmed/36850337
http://dx.doi.org/10.3390/polym15041054
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author Fatiroi, Nurul Syazwanie
Jaziri, Abdul Aziz
Shapawi, Rossita
Mokhtar, Ruzaidi Azli Mohd
Noordin, Wan Norhana Md.
Huda, Nurul
author_facet Fatiroi, Nurul Syazwanie
Jaziri, Abdul Aziz
Shapawi, Rossita
Mokhtar, Ruzaidi Azli Mohd
Noordin, Wan Norhana Md.
Huda, Nurul
author_sort Fatiroi, Nurul Syazwanie
collection PubMed
description Biopolymer-like collagen has great industrial potential in terms of its excellent properties, such as strong biocompatibility, high degradability, and low antigenicity. Collagen derived from fish by-products is preferable as it is safer (free from transmittable diseases) and acceptable to most religious beliefs. This study aimed to characterize the unicornfish (Naso reticulatus Randall, 2001) bone collagens prepared with different type of acids, i.e., acetic acid, lactic acid, and citric acid. A higher yield (Y) (p < 0.05) was obtained in the citric-acid-soluble collagen (CASC) (Y = 1.36%), followed by the lactic-acid-soluble collagen (LASC) (Y = 1.08%) and acetic-acid-soluble collagen (AASC) (Y = 0.40%). All extracted collagens were classified as type I due to the presence of 2-alpha chains (α1 and α2). Their prominent absorption spectra were located at the wavelengths of 229.83 nm to 231.17 nm. This is similar to wavelengths reported for other fish collagens. The X-ray diffraction (XRD) and infrared (IR) data demonstrated that the triple-helical structure of type I collagens was still preserved after the acid-extraction process. In terms of thermal stability, all samples had similar maximum transition temperatures (T(max) = 33.34–33.51 °C). A higher relative solubility (RS) of the unicornfish bone collagens was observed at low salt concentration (0–10 g/L) (RS > 80%) and at acidic condition (pH 1.0 to pH 3.0) (RS > 75%). The extracted collagen samples had an irregular and dense flake structure with random coiled filaments. Overall, bones of unicornfish may be used as a substitute source of collagen.
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spelling pubmed-99647612023-02-26 Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types Fatiroi, Nurul Syazwanie Jaziri, Abdul Aziz Shapawi, Rossita Mokhtar, Ruzaidi Azli Mohd Noordin, Wan Norhana Md. Huda, Nurul Polymers (Basel) Article Biopolymer-like collagen has great industrial potential in terms of its excellent properties, such as strong biocompatibility, high degradability, and low antigenicity. Collagen derived from fish by-products is preferable as it is safer (free from transmittable diseases) and acceptable to most religious beliefs. This study aimed to characterize the unicornfish (Naso reticulatus Randall, 2001) bone collagens prepared with different type of acids, i.e., acetic acid, lactic acid, and citric acid. A higher yield (Y) (p < 0.05) was obtained in the citric-acid-soluble collagen (CASC) (Y = 1.36%), followed by the lactic-acid-soluble collagen (LASC) (Y = 1.08%) and acetic-acid-soluble collagen (AASC) (Y = 0.40%). All extracted collagens were classified as type I due to the presence of 2-alpha chains (α1 and α2). Their prominent absorption spectra were located at the wavelengths of 229.83 nm to 231.17 nm. This is similar to wavelengths reported for other fish collagens. The X-ray diffraction (XRD) and infrared (IR) data demonstrated that the triple-helical structure of type I collagens was still preserved after the acid-extraction process. In terms of thermal stability, all samples had similar maximum transition temperatures (T(max) = 33.34–33.51 °C). A higher relative solubility (RS) of the unicornfish bone collagens was observed at low salt concentration (0–10 g/L) (RS > 80%) and at acidic condition (pH 1.0 to pH 3.0) (RS > 75%). The extracted collagen samples had an irregular and dense flake structure with random coiled filaments. Overall, bones of unicornfish may be used as a substitute source of collagen. MDPI 2023-02-20 /pmc/articles/PMC9964761/ /pubmed/36850337 http://dx.doi.org/10.3390/polym15041054 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fatiroi, Nurul Syazwanie
Jaziri, Abdul Aziz
Shapawi, Rossita
Mokhtar, Ruzaidi Azli Mohd
Noordin, Wan Norhana Md.
Huda, Nurul
Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title_full Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title_fullStr Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title_full_unstemmed Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title_short Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso reticulatus Randall, 2001) Bone Prepared with Various Acid Types
title_sort biochemical and microstructural characteristics of collagen biopolymer from unicornfish (naso reticulatus randall, 2001) bone prepared with various acid types
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964761/
https://www.ncbi.nlm.nih.gov/pubmed/36850337
http://dx.doi.org/10.3390/polym15041054
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