Cargando…

Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion

Domestic ruminant abortions due to infectious agents represent an important cause of economic losses in the agricultural industry. This study aimed to optimise and apply qPCR assays for detection of Chlamydiales in domestic ruminant abortion cases. Primers and probes for detection of the order Chlam...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jonker, Annelize, Michel, Anita L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965055/ https://www.ncbi.nlm.nih.gov/pubmed/36839562 http://dx.doi.org/10.3390/pathogens12020290

Ejemplares similares

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants
por: Jonker, Annelize, et al.
Publicado: (2023)

Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands
por: Zhang, Jilei, et al.
Publicado: (2015)

Data on the optimization of an archaea-specific probe-based qPCR assay
por: Bomberg, Malin, et al.
Publicado: (2020)

Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum
por: da Silva, Bianca Kelly Neves Izidro, et al.
Publicado: (2023)

Artificial Substrates Coupled with qPCR (AS-qPCR) Assay for the Detection of the Toxic Benthopelagic Dinoflagellate Vulcanodinium rugosum
por: Bouquet, Aurélien, et al.
Publicado: (2023)

Technical Evaluation of qPCR Multiplex Assays for the Detection of Ixodes ricinus-Borne Pathogens
por: Azagi, Tal, et al.
Publicado: (2022)

TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
por: Zhang, Qian, et al.
Publicado: (2015)

MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay
por: Nagy, Alexander, et al.
Publicado: (2016)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

Correction: MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay
por: Nagy, Alexander, et al.
Publicado: (2018)

Sample-ready multiplex qPCR assay for detection of malaria
por: Kamau, Edwin, et al.
Publicado: (2014)

Postmenopausal Osteoporosis reference genes for qPCR expression assays
por: de Lima, Camilla Albertina Dantas, et al.
Publicado: (2019)

An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections
por: Sazed, Saiful Arefeen, et al.
Publicado: (2021)

PCR-Stop analysis as a new tool for qPCR assay validation
por: Witte, Anna Kristina, et al.
Publicado: (2018)

Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
por: Yang, Ting‐bang, et al.
Publicado: (2020)

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats
por: Modise, Boitumelo M., et al.
Publicado: (2023)

Prevalence and diversity of Chlamydiales and other amoeba-resisting bacteria in domestic drinking water systems
por: Lienard, J., et al.
Publicado: (2016)

A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach
por: Mei, Qian, et al.
Publicado: (2012)

The ‘PREXCEL-Q Method’ for qPCR
por: Gallup, Jack M., et al.
Publicado: (2008)

Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta
por: Rueda-Martínez, Carmen, et al.
Publicado: (2014)

Comparison of qPCR and culture methods for group B Streptococcus colonization detection in pregnant women: evaluation of a new qPCR assay
por: Carrillo-Ávila, J. A., et al.
Publicado: (2018)

Simultaneous Detection of Ricin and Abrin DNA by Real-Time PCR (qPCR)
por: Felder, Eva, et al.
Publicado: (2012)

Probe-Based Real-Time qPCR Assays for a Reliable Differentiation of Capripox Virus Species
por: Wolff, Janika, et al.
Publicado: (2021)

Development of a Genus-Specific Brucella Real-Time PCR Assay Targeting the 16S-23S rDNA Internal Transcribed Spacer from Different Specimen Types
por: Nyarku, Rejoice, et al.
Publicado: (2020)

Evaluation of Various Campylobacter-Specific Quantitative PCR (qPCR) Assays for Detection and Enumeration of Campylobacteraceae in Irrigation Water and Wastewater via a Miniaturized Most-Probable-Number–qPCR Assay
por: Banting, Graham S., et al.
Publicado: (2016)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae
por: Saad, Osama S., et al.
Publicado: (2020)

A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
por: Kamber, Tim, et al.
Publicado: (2021)

A qPCR-duplex assay for sex determination in ancient DNA
por: Poma, Anna, et al.
Publicado: (2022)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

BlotIt—Optimal alignment of Western blot and qPCR experiments
por: Kemmer, Svenja, et al.
Publicado: (2022)

The Development of a Novel qPCR Assay-Set for Identifying Fecal Contamination Originating from Domestic Fowls and Waterfowl in Israel
por: Ohad, Shoshanit, et al.
Publicado: (2016)

MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
por: Staggemeier, Rodrigo, et al.
Publicado: (2014)

Performance Characteristics of qPCR Assays Targeting Human- and Ruminant-Associated Bacteroidetes for Microbial Source Tracking across Sixteen Countries on Six Continents
por: Reischer, Georg H., et al.
Publicado: (2013)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
por: Li, Baowei, et al.
Publicado: (2021)

Multiplex Real-Time qPCR Assay for Simultaneous and Sensitive Detection of Phytoplasmas in Sesame Plants and Insect Vectors
por: Ikten, Cengiz, et al.
Publicado: (2016)

Development of real-time reverse transcriptase qPCR assays for the detection of Punta Toro virus and Pichinde virus
por: Stefan, Christopher P., et al.
Publicado: (2016)

Evaluation of qPCR-Based Assays for Leprosy Diagnosis Directly in Clinical Specimens
por: Martinez, Alejandra Nóbrega, et al.
Publicado: (2011)

A New qPCR Assay for the Rapid Diagnosis of Anthonomus grandis Subspecies
por: Raszick, Tyler Jay, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_uq54ghkc2u7hfli0hk2sfj835u