Cargando…

Optimized Fast Filtration-Based Sampling and Extraction Enables Precise and Absolute Quantification of the Escherichia coli Central Carbon Metabolome

Precise and accurate quantification is a prerequisite for interpretation of targeted metabolomics data, but this task is challenged by the inherent instability of the analytes. The sampling, quenching, extraction, and sample purification conditions required to recover and stabilize metabolites in re...

Descripción completa

Detalles Bibliográficos
Autores principales:	Thorfinnsdottir, Lilja Brekke, García-Calvo, Laura, Bø, Gaute Hovde, Bruheim, Per, Røst, Lisa Marie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965072/ https://www.ncbi.nlm.nih.gov/pubmed/36837769 http://dx.doi.org/10.3390/metabo13020150

Ejemplares similares

Survival of Escherichia coli after high-antibiotic stress is dependent on both the pregrown physiological state and incubation conditions
por: Thorfinnsdottir, Lilja Brekke, et al.
Publicado: (2023)

Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems
por: Røst, Lisa M., et al.
Publicado: (2020)

Experimental determination of Escherichia coli biomass composition for constraint-based metabolic modeling
por: Simensen, Vetle, et al.
Publicado: (2022)

Nutrient-depended metabolic switching during batch cultivation of Streptomyces coelicolor explored with absolute quantitative mass spectrometry-based metabolite profiling
por: Kumar, Kanhaiya, et al.
Publicado: (2022)

A method for metabolomic sampling of suspended animal cells using fast filtration
por: Volmer, Martin, et al.
Publicado: (2011)

Fast Filtration of Bacterial or Mammalian Suspension Cell Cultures for Optimal Metabolomics Results
por: Bordag, Natalie, et al.
Publicado: (2016)

Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers
por: Oliveira, Beatriz, et al.
Publicado: (2020)

Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification
por: Tao, Hui, et al.
Publicado: (2016)

Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression
por: Pinto, Rita, et al.
Publicado: (2017)

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards
por: Rzagalinski, Ignacy, et al.
Publicado: (2022)

SCALiR: a web application for automating absolute quantification of mass spectrometry-based metabolomics data
por: Bishop, Stephanie L., et al.
Publicado: (2023)

Proton NMR Enables the Absolute Quantification of Aqueous Metabolites and Lipid Classes in Unique Mouse Liver Samples
por: Amiel, Aurélien, et al.
Publicado: (2019)

Fast MS/MS acquisition without dynamic exclusion enables precise and accurate quantification of proteome by MS/MS fragment intensity
por: Zhang, Shen, et al.
Publicado: (2016)

A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification
por: Rutledge, Robert G.
Publicado: (2011)

Metabolomics enables precision medicine: “A White Paper, Community Perspective”
por: Beger, Richard D., et al.
Publicado: (2016)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Absolute Quantification of Endogenous Ras Isoform Abundance
por: Mageean, Craig J., et al.
Publicado: (2015)

Absolute Quantification of Amyloid Propagons by Digital Microfluidics
por: Pfammatter, Manuela, et al.
Publicado: (2017)

Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
por: Jacob, Dominik, et al.
Publicado: (2019)

Absolute Quantification of Drug Vector Delivery to the Cytosol
por: Lucchino, Marco, et al.
Publicado: (2021)

Digital Encoding of Cellular mRNAs Enabling Precise and Absolute Gene Expression Measurement by Single-Molecule Counting
por: Fu, Glenn K., et al.
Publicado: (2014)

Cross-Flow Filtration of Escherichia coli at a Nanofluidic Gap for Fast Immobilization and Antibiotic Susceptibility Testing
por: Busche, Jan F., et al.
Publicado: (2019)

Synthetic Gene Circuit-Based Assay with Multilevel Switch Enables Background-Free and Absolute Quantification of Circulating Tumor DNA
por: Zhang, Chao, et al.
Publicado: (2023)

Multiplex qPCR for Detection and Absolute Quantification of Malaria
por: Kamau, Edwin, et al.
Publicado: (2013)

Absolute Quantification of Selected Proteins in the Human Osteoarthritic Secretome
por: Peffers, Mandy J., et al.
Publicado: (2013)

Absolute quantification of somatic DNA alterations in human cancer
por: Carter, Scott L., et al.
Publicado: (2012)

Absolute quantification of cohesin, CTCF and their regulators in human cells
por: Holzmann, Johann, et al.
Publicado: (2019)

Feasibility of absolute quantification for (31)P MRS at 7 T
por: Purvis, Lucian A. B., et al.
Publicado: (2019)

Absolute Quantification in Diagnostic SPECT/CT: The Phantom Premise
por: De Schepper, Stijn, et al.
Publicado: (2021)

Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling
por: Jang, Mi, et al.
Publicado: (2022)

High-Precision Absolute Pose Sensing for Parallel Mechanisms
por: Schempp, Constantin, et al.
Publicado: (2022)

The Tumor Necrosis Factor Alpha and Interleukin 6 Auto-paracrine Signaling Loop Controls Mycobacterium avium Infection via Induction of IRF1/IRG1 in Human Primary Macrophages
por: Gidon, Alexandre, et al.
Publicado: (2021)

Pyruvate Supports RET-Dependent Mitochondrial ROS Production to Control Mycobacterium avium Infection in Human Primary Macrophages
por: Røst, Lisa Marie, et al.
Publicado: (2022)

Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT)
por: Nakano, Dan, et al.
Publicado: (2020)

Absolute Quantification of Individual Biomass Concentrations in a Methanogenic Coculture
por: Junicke, Helena, et al.
Publicado: (2014)

Absolute and relative quantification of RNA modifications via biosynthetic isotopomers
por: Kellner, Stefanie, et al.
Publicado: (2014)

Absolute quantification of cell-free microRNAs in cancer patients
por: Ferracin, Manuela, et al.
Publicado: (2015)

Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR
por: Thapa, Punam, et al.
Publicado: (2022)

Absolute Proteome Quantification in the Gas-Fermenting Acetogen Clostridium autoethanogenum
por: Valgepea, Kaspar, et al.
Publicado: (2022)

Absolute protein quantification using fluorescence measurements with FPCountR
por: Csibra, Eszter, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_me6ov013tiv489m0rgno38q21n