Cargando…

Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications

Recent studies in experimental animals found that oral exposure to micro- and nano-plastics (MNPs) during pregnancy had multiple adverse effects on outcomes and progeny, although no study has yet identified the translocation of ingested MNPs to the placenta or fetal tissues, which might account for...

Descripción completa

Detalles Bibliográficos
Autores principales: Cary, Chelsea M., DeLoid, Glen M., Yang, Zhenning, Bitounis, Dimitrios, Polunas, Marianne, Goedken, Michael J., Buckley, Brian, Cheatham, Byron, Stapleton, Phoebe A., Demokritou, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965230/
https://www.ncbi.nlm.nih.gov/pubmed/36839088
http://dx.doi.org/10.3390/nano13040720
_version_ 1784896706325774336
author Cary, Chelsea M.
DeLoid, Glen M.
Yang, Zhenning
Bitounis, Dimitrios
Polunas, Marianne
Goedken, Michael J.
Buckley, Brian
Cheatham, Byron
Stapleton, Phoebe A.
Demokritou, Philip
author_facet Cary, Chelsea M.
DeLoid, Glen M.
Yang, Zhenning
Bitounis, Dimitrios
Polunas, Marianne
Goedken, Michael J.
Buckley, Brian
Cheatham, Byron
Stapleton, Phoebe A.
Demokritou, Philip
author_sort Cary, Chelsea M.
collection PubMed
description Recent studies in experimental animals found that oral exposure to micro- and nano-plastics (MNPs) during pregnancy had multiple adverse effects on outcomes and progeny, although no study has yet identified the translocation of ingested MNPs to the placenta or fetal tissues, which might account for those effects. We therefore assessed the placental and fetal translocation of ingested nanoscale polystyrene MNPs in pregnant rats. Sprague Dawley rats (N = 5) were gavaged on gestational day 19 with 10 mL/kg of 250 µg/mL 25 nm carboxylated polystyrene spheres (PS25C) and sacrificed after 24 h. Hyperspectral imaging of harvested placental and fetal tissues identified abundant PS25C within the placenta and in all fetal tissues examined, including liver, kidney, heart, lung and brain, where they appeared in 10–25 µm clusters. These findings demonstrate that ingested nanoscale polystyrene MNPs can breach the intestinal barrier and subsequently the maternal–fetal barrier of the placenta to access the fetal circulation and all fetal tissues. Further studies are needed to assess the mechanisms of MNP translocation across the intestinal and placental barriers, the effects of MNP polymer, size and other physicochemical properties on translocation, as well as the potential adverse effects of MNP translocation on the developing fetus.
format Online
Article
Text
id pubmed-9965230
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99652302023-02-26 Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications Cary, Chelsea M. DeLoid, Glen M. Yang, Zhenning Bitounis, Dimitrios Polunas, Marianne Goedken, Michael J. Buckley, Brian Cheatham, Byron Stapleton, Phoebe A. Demokritou, Philip Nanomaterials (Basel) Article Recent studies in experimental animals found that oral exposure to micro- and nano-plastics (MNPs) during pregnancy had multiple adverse effects on outcomes and progeny, although no study has yet identified the translocation of ingested MNPs to the placenta or fetal tissues, which might account for those effects. We therefore assessed the placental and fetal translocation of ingested nanoscale polystyrene MNPs in pregnant rats. Sprague Dawley rats (N = 5) were gavaged on gestational day 19 with 10 mL/kg of 250 µg/mL 25 nm carboxylated polystyrene spheres (PS25C) and sacrificed after 24 h. Hyperspectral imaging of harvested placental and fetal tissues identified abundant PS25C within the placenta and in all fetal tissues examined, including liver, kidney, heart, lung and brain, where they appeared in 10–25 µm clusters. These findings demonstrate that ingested nanoscale polystyrene MNPs can breach the intestinal barrier and subsequently the maternal–fetal barrier of the placenta to access the fetal circulation and all fetal tissues. Further studies are needed to assess the mechanisms of MNP translocation across the intestinal and placental barriers, the effects of MNP polymer, size and other physicochemical properties on translocation, as well as the potential adverse effects of MNP translocation on the developing fetus. MDPI 2023-02-14 /pmc/articles/PMC9965230/ /pubmed/36839088 http://dx.doi.org/10.3390/nano13040720 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cary, Chelsea M.
DeLoid, Glen M.
Yang, Zhenning
Bitounis, Dimitrios
Polunas, Marianne
Goedken, Michael J.
Buckley, Brian
Cheatham, Byron
Stapleton, Phoebe A.
Demokritou, Philip
Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title_full Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title_fullStr Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title_full_unstemmed Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title_short Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications
title_sort ingested polystyrene nanospheres translocate to placenta and fetal tissues in pregnant rats: potential health implications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965230/
https://www.ncbi.nlm.nih.gov/pubmed/36839088
http://dx.doi.org/10.3390/nano13040720
work_keys_str_mv AT carychelseam ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT deloidglenm ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT yangzhenning ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT bitounisdimitrios ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT polunasmarianne ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT goedkenmichaelj ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT buckleybrian ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT cheathambyron ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT stapletonphoebea ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications
AT demokritouphilip ingestedpolystyrenenanospherestranslocatetoplacentaandfetaltissuesinpregnantratspotentialhealthimplications