Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

SIMPLE SUMMARY: Insect cell lines have often been employed in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review summarizes the progression of insect cell line establishment, then introduces several recent studies based on insect cell lines coupled with advanced technologies. These cell-based studies could increase efficiency and reduce the cost of insecticide studies, providing a global and in-depth perspective to reveal and verify the mode of action of insecticides. ABSTRACT: During the past decades, research on insect cell culture has grown tremendously. Thousands of lines have been established from different species of insect orders, originating from several tissue sources. These cell lines have often been employed in insect science research. In particular, they have played important roles in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review intends to first briefly summarize the progression of insect cell line establishment. Then, several recent studies based on insect cell lines coupled with advanced technologies are introduced. These investigations revealed that insect cell lines can be exploited as novel models with unique advantages such as increased efficiency and reduced cost compared with traditional insecticide research. Most notably, the insect cell line-based models provide a global and in-depth perspective to study the toxicology mechanisms of insecticides. However, challenges and limitations still exist, especially in the connection between in vitro activity and in vivo effectiveness. Despite all this, recent advances have suggested that insect cell line-based models promote the progress and sensible application of insecticides, which benefits pest management.