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Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells

Carbon nanotubes (CNTs) possess excellent physicochemical and structural properties alongside their nano dimensions, constituting a medical platform for the delivery of different therapeutic molecules and drug systems. Hydroxytyrosol (HT) is a molecule with potent antioxidant properties that, howeve...

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Autores principales: Zygouri, Panagiota, Athinodorou, Antrea M., Spyrou, Konstantinos, Simos, Yannis V., Subrati, Mohammed, Asimakopoulos, Georgios, Vasilopoulos, Konstantinos C., Vezyraki, Patra, Peschos, Dimitrios, Tsamis, Konstantinos, Gournis, Dimitrios P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965370/
https://www.ncbi.nlm.nih.gov/pubmed/36839082
http://dx.doi.org/10.3390/nano13040714
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author Zygouri, Panagiota
Athinodorou, Antrea M.
Spyrou, Konstantinos
Simos, Yannis V.
Subrati, Mohammed
Asimakopoulos, Georgios
Vasilopoulos, Konstantinos C.
Vezyraki, Patra
Peschos, Dimitrios
Tsamis, Konstantinos
Gournis, Dimitrios P.
author_facet Zygouri, Panagiota
Athinodorou, Antrea M.
Spyrou, Konstantinos
Simos, Yannis V.
Subrati, Mohammed
Asimakopoulos, Georgios
Vasilopoulos, Konstantinos C.
Vezyraki, Patra
Peschos, Dimitrios
Tsamis, Konstantinos
Gournis, Dimitrios P.
author_sort Zygouri, Panagiota
collection PubMed
description Carbon nanotubes (CNTs) possess excellent physicochemical and structural properties alongside their nano dimensions, constituting a medical platform for the delivery of different therapeutic molecules and drug systems. Hydroxytyrosol (HT) is a molecule with potent antioxidant properties that, however, is rapidly metabolized in the organism. HT immobilized on functionalized CNTs could improve its oral absorption and protect it against rapid degradation and elimination. This study investigated the effects of cellular oxidized multiwall carbon nanotubes (oxMWCNTs) as biocompatible carriers of HT. The oxidation of MWCNTs via H(2)SO(4) and HNO(3) has a double effect since it leads to increased hydrophilicity, while the introduced oxygen functionalities can contribute to the delivery of the drug. The in vitro effects of HT, oxMWCNTS, and oxMWCNTS functionalized with HT (oxMWCNTS_HT) were studied against two different cell lines (NIH/3T3 and Tg/Tg). We evaluated the toxicity (MTT and clonogenic assay), cell cycle arrest, and reactive oxygen species (ROS) formation. Both cell lines coped with oxMWCNTs even at high doses. oxMWCNTS_HT acted as pro-oxidants in Tg/Tg cells and as antioxidants in NIH/3T3 cells. These findings suggest that oxMWCNTs could evolve into a promising nanocarrier suitable for targeted drug delivery in the future.
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spelling pubmed-99653702023-02-26 Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells Zygouri, Panagiota Athinodorou, Antrea M. Spyrou, Konstantinos Simos, Yannis V. Subrati, Mohammed Asimakopoulos, Georgios Vasilopoulos, Konstantinos C. Vezyraki, Patra Peschos, Dimitrios Tsamis, Konstantinos Gournis, Dimitrios P. Nanomaterials (Basel) Article Carbon nanotubes (CNTs) possess excellent physicochemical and structural properties alongside their nano dimensions, constituting a medical platform for the delivery of different therapeutic molecules and drug systems. Hydroxytyrosol (HT) is a molecule with potent antioxidant properties that, however, is rapidly metabolized in the organism. HT immobilized on functionalized CNTs could improve its oral absorption and protect it against rapid degradation and elimination. This study investigated the effects of cellular oxidized multiwall carbon nanotubes (oxMWCNTs) as biocompatible carriers of HT. The oxidation of MWCNTs via H(2)SO(4) and HNO(3) has a double effect since it leads to increased hydrophilicity, while the introduced oxygen functionalities can contribute to the delivery of the drug. The in vitro effects of HT, oxMWCNTS, and oxMWCNTS functionalized with HT (oxMWCNTS_HT) were studied against two different cell lines (NIH/3T3 and Tg/Tg). We evaluated the toxicity (MTT and clonogenic assay), cell cycle arrest, and reactive oxygen species (ROS) formation. Both cell lines coped with oxMWCNTs even at high doses. oxMWCNTS_HT acted as pro-oxidants in Tg/Tg cells and as antioxidants in NIH/3T3 cells. These findings suggest that oxMWCNTs could evolve into a promising nanocarrier suitable for targeted drug delivery in the future. MDPI 2023-02-13 /pmc/articles/PMC9965370/ /pubmed/36839082 http://dx.doi.org/10.3390/nano13040714 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zygouri, Panagiota
Athinodorou, Antrea M.
Spyrou, Konstantinos
Simos, Yannis V.
Subrati, Mohammed
Asimakopoulos, Georgios
Vasilopoulos, Konstantinos C.
Vezyraki, Patra
Peschos, Dimitrios
Tsamis, Konstantinos
Gournis, Dimitrios P.
Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title_full Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title_fullStr Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title_full_unstemmed Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title_short Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells
title_sort oxidized-multiwalled carbon nanotubes as non-toxic nanocarriers for hydroxytyrosol delivery in cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965370/
https://www.ncbi.nlm.nih.gov/pubmed/36839082
http://dx.doi.org/10.3390/nano13040714
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