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Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial lung disease of unknown etiology. At present, the mortality rate of the deadly disease is still very high, while the existing treatments only delay the progression of the disease and improve the quality of life of p...

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Autores principales: Yu, Rui, Gao, Dandi, Bao, Jiali, Sun, Ronghao, Cui, Mengqi, Mao, Yunyun, Li, Kai, Hu, Enbo, Zhai, Yanfang, Liu, Yanhong, Gao, Yuemei, Xiao, Ting, Zhou, Honggang, Yang, Cheng, Xu, Junjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965428/
https://www.ncbi.nlm.nih.gov/pubmed/36835236
http://dx.doi.org/10.3390/ijms24043818
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author Yu, Rui
Gao, Dandi
Bao, Jiali
Sun, Ronghao
Cui, Mengqi
Mao, Yunyun
Li, Kai
Hu, Enbo
Zhai, Yanfang
Liu, Yanhong
Gao, Yuemei
Xiao, Ting
Zhou, Honggang
Yang, Cheng
Xu, Junjie
author_facet Yu, Rui
Gao, Dandi
Bao, Jiali
Sun, Ronghao
Cui, Mengqi
Mao, Yunyun
Li, Kai
Hu, Enbo
Zhai, Yanfang
Liu, Yanhong
Gao, Yuemei
Xiao, Ting
Zhou, Honggang
Yang, Cheng
Xu, Junjie
author_sort Yu, Rui
collection PubMed
description Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial lung disease of unknown etiology. At present, the mortality rate of the deadly disease is still very high, while the existing treatments only delay the progression of the disease and improve the quality of life of patients. Lung cancer (LC) is the most fatal disease in the world. In recent years, IPF has been considered to be an independent risk factor for the development of LC. The incidence of lung cancer is increased in the patients with IPF and the mortality is also significantly increased in the patients inflicted with the two diseases. In this study, we evaluated an animal model of pulmonary fibrosis complicated with LC by implanting LC cells orthotopically into the lungs of mice several days after bleomycin induction of the pulmonary fibrosis in the same mice. In vivo studies with the model showed that exogenous recombinant human thymosin beta 4 (exo-rhTβ4) alleviated the impairment of lung function and severity of damage of the alveolar structure by the pulmonary fibrosis and inhibited the proliferation of LC tumor growth. In addition, in vitro studies showed that exo-rhTβ4 inhibited the proliferation and migration of A549 and Mlg cells. Furthermore, our results also showed that rhTβ4 could effectively inhibit the JAK2-STAT3 signaling pathway and this might exert an anti-IPF-LC effect. The establishment of the IPF-LC animal model will be helpful for the development of drugs for the treatment of IPF-LC. Exogenous rhTβ4 can be potentially used for the treatment of IPF and LC.
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spelling pubmed-99654282023-02-26 Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway Yu, Rui Gao, Dandi Bao, Jiali Sun, Ronghao Cui, Mengqi Mao, Yunyun Li, Kai Hu, Enbo Zhai, Yanfang Liu, Yanhong Gao, Yuemei Xiao, Ting Zhou, Honggang Yang, Cheng Xu, Junjie Int J Mol Sci Article Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial lung disease of unknown etiology. At present, the mortality rate of the deadly disease is still very high, while the existing treatments only delay the progression of the disease and improve the quality of life of patients. Lung cancer (LC) is the most fatal disease in the world. In recent years, IPF has been considered to be an independent risk factor for the development of LC. The incidence of lung cancer is increased in the patients with IPF and the mortality is also significantly increased in the patients inflicted with the two diseases. In this study, we evaluated an animal model of pulmonary fibrosis complicated with LC by implanting LC cells orthotopically into the lungs of mice several days after bleomycin induction of the pulmonary fibrosis in the same mice. In vivo studies with the model showed that exogenous recombinant human thymosin beta 4 (exo-rhTβ4) alleviated the impairment of lung function and severity of damage of the alveolar structure by the pulmonary fibrosis and inhibited the proliferation of LC tumor growth. In addition, in vitro studies showed that exo-rhTβ4 inhibited the proliferation and migration of A549 and Mlg cells. Furthermore, our results also showed that rhTβ4 could effectively inhibit the JAK2-STAT3 signaling pathway and this might exert an anti-IPF-LC effect. The establishment of the IPF-LC animal model will be helpful for the development of drugs for the treatment of IPF-LC. Exogenous rhTβ4 can be potentially used for the treatment of IPF and LC. MDPI 2023-02-14 /pmc/articles/PMC9965428/ /pubmed/36835236 http://dx.doi.org/10.3390/ijms24043818 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Rui
Gao, Dandi
Bao, Jiali
Sun, Ronghao
Cui, Mengqi
Mao, Yunyun
Li, Kai
Hu, Enbo
Zhai, Yanfang
Liu, Yanhong
Gao, Yuemei
Xiao, Ting
Zhou, Honggang
Yang, Cheng
Xu, Junjie
Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title_full Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title_fullStr Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title_full_unstemmed Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title_short Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway
title_sort exogenous thymosin beta 4 suppresses ipf-lung cancer in mice: possibly associated with its inhibitory effect on the jak2/stat3 signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965428/
https://www.ncbi.nlm.nih.gov/pubmed/36835236
http://dx.doi.org/10.3390/ijms24043818
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