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Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato

Verticillium dahliae is a soil-borne pathogenic fungus that causes Verticillium wilt in host plants, a particularly serious problem in potato cultivation. Several pathogenicity-related proteins play important roles in the host infection process, hence, identifying such proteins, especially those wit...

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Autores principales: Wang, Dahui, Wen, Shenglan, Zhao, Zhibo, Long, Youhua, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965449/
https://www.ncbi.nlm.nih.gov/pubmed/36835042
http://dx.doi.org/10.3390/ijms24043630
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author Wang, Dahui
Wen, Shenglan
Zhao, Zhibo
Long, Youhua
Fan, Rong
author_facet Wang, Dahui
Wen, Shenglan
Zhao, Zhibo
Long, Youhua
Fan, Rong
author_sort Wang, Dahui
collection PubMed
description Verticillium dahliae is a soil-borne pathogenic fungus that causes Verticillium wilt in host plants, a particularly serious problem in potato cultivation. Several pathogenicity-related proteins play important roles in the host infection process, hence, identifying such proteins, especially those with unknown functions, will surely aid in understanding the mechanism responsible for the pathogenesis of the fungus. Here, tandem mass tag (TMT) was used to quantitatively analyze the differentially expressed proteins in V. dahliae during the infection of the susceptible potato cultivar “Favorita”. Potato seedlings were infected with V. dahliae and incubated for 36 h, after which 181 proteins were found to be significantly upregulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that most of these proteins were involved in early growth and cell wall degradation. The hypothetical, secretory protein with an unknown function, VDAG_07742, was significantly upregulated during infection. The functional analysis with knockout and complementation mutants revealed that the associated gene was not involved in mycelial growth, conidial production, or germination; however, the penetration ability and pathogenicity of VDAG_07742 deletion mutants were significantly reduced. Therefore, our results strongly indicate that VDAG_07742 is essential in the early stage of potato infection by V. dahliae.
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spelling pubmed-99654492023-02-26 Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato Wang, Dahui Wen, Shenglan Zhao, Zhibo Long, Youhua Fan, Rong Int J Mol Sci Article Verticillium dahliae is a soil-borne pathogenic fungus that causes Verticillium wilt in host plants, a particularly serious problem in potato cultivation. Several pathogenicity-related proteins play important roles in the host infection process, hence, identifying such proteins, especially those with unknown functions, will surely aid in understanding the mechanism responsible for the pathogenesis of the fungus. Here, tandem mass tag (TMT) was used to quantitatively analyze the differentially expressed proteins in V. dahliae during the infection of the susceptible potato cultivar “Favorita”. Potato seedlings were infected with V. dahliae and incubated for 36 h, after which 181 proteins were found to be significantly upregulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that most of these proteins were involved in early growth and cell wall degradation. The hypothetical, secretory protein with an unknown function, VDAG_07742, was significantly upregulated during infection. The functional analysis with knockout and complementation mutants revealed that the associated gene was not involved in mycelial growth, conidial production, or germination; however, the penetration ability and pathogenicity of VDAG_07742 deletion mutants were significantly reduced. Therefore, our results strongly indicate that VDAG_07742 is essential in the early stage of potato infection by V. dahliae. MDPI 2023-02-11 /pmc/articles/PMC9965449/ /pubmed/36835042 http://dx.doi.org/10.3390/ijms24043630 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Dahui
Wen, Shenglan
Zhao, Zhibo
Long, Youhua
Fan, Rong
Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title_full Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title_fullStr Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title_full_unstemmed Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title_short Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
title_sort hypothetical protein vdag_07742 is required for verticillium dahliae pathogenicity in potato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965449/
https://www.ncbi.nlm.nih.gov/pubmed/36835042
http://dx.doi.org/10.3390/ijms24043630
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