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Nanobiotechnology-Enabled mRNA Stabilization
mRNA technology has attracted enormous interest due to its great therapeutic potential. Strategies that can stabilize fragile mRNA molecules are crucial for their widespread applications. There are numerous reviews on mRNA delivery, but few focus on the underlying causes of mRNA instability and how...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965532/ https://www.ncbi.nlm.nih.gov/pubmed/36839942 http://dx.doi.org/10.3390/pharmaceutics15020620 |
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author | Xian, He Zhang, Yue Yu, Chengzhong Wang, Yue |
author_facet | Xian, He Zhang, Yue Yu, Chengzhong Wang, Yue |
author_sort | Xian, He |
collection | PubMed |
description | mRNA technology has attracted enormous interest due to its great therapeutic potential. Strategies that can stabilize fragile mRNA molecules are crucial for their widespread applications. There are numerous reviews on mRNA delivery, but few focus on the underlying causes of mRNA instability and how to tackle the instability issues. Herein, the recent progress in nanobiotechnology-enabled strategies for stabilizing mRNA and better delivery is reviewed. First, factors that destabilize mRNA are introduced. Second, nanobiotechnology-enabled strategies to stabilize mRNA molecules are reviewed, including molecular and nanotechnology approaches. The impact of formulation processing on mRNA stability and shelf-life, including freezing and lyophilization, are also briefly discussed. Lastly, our perspectives on challenges and future directions are presented. This review may provide useful guidelines for understanding the structure–function relationship and the rational design of nanobiotechnology for mRNA stability enhancement and mRNA technology development. |
format | Online Article Text |
id | pubmed-9965532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99655322023-02-26 Nanobiotechnology-Enabled mRNA Stabilization Xian, He Zhang, Yue Yu, Chengzhong Wang, Yue Pharmaceutics Review mRNA technology has attracted enormous interest due to its great therapeutic potential. Strategies that can stabilize fragile mRNA molecules are crucial for their widespread applications. There are numerous reviews on mRNA delivery, but few focus on the underlying causes of mRNA instability and how to tackle the instability issues. Herein, the recent progress in nanobiotechnology-enabled strategies for stabilizing mRNA and better delivery is reviewed. First, factors that destabilize mRNA are introduced. Second, nanobiotechnology-enabled strategies to stabilize mRNA molecules are reviewed, including molecular and nanotechnology approaches. The impact of formulation processing on mRNA stability and shelf-life, including freezing and lyophilization, are also briefly discussed. Lastly, our perspectives on challenges and future directions are presented. This review may provide useful guidelines for understanding the structure–function relationship and the rational design of nanobiotechnology for mRNA stability enhancement and mRNA technology development. MDPI 2023-02-12 /pmc/articles/PMC9965532/ /pubmed/36839942 http://dx.doi.org/10.3390/pharmaceutics15020620 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Xian, He Zhang, Yue Yu, Chengzhong Wang, Yue Nanobiotechnology-Enabled mRNA Stabilization |
title | Nanobiotechnology-Enabled mRNA Stabilization |
title_full | Nanobiotechnology-Enabled mRNA Stabilization |
title_fullStr | Nanobiotechnology-Enabled mRNA Stabilization |
title_full_unstemmed | Nanobiotechnology-Enabled mRNA Stabilization |
title_short | Nanobiotechnology-Enabled mRNA Stabilization |
title_sort | nanobiotechnology-enabled mrna stabilization |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965532/ https://www.ncbi.nlm.nih.gov/pubmed/36839942 http://dx.doi.org/10.3390/pharmaceutics15020620 |
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