Cargando…
LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965601/ https://www.ncbi.nlm.nih.gov/pubmed/36835138 http://dx.doi.org/10.3390/ijms24043728 |
_version_ | 1784896805723439104 |
---|---|
author | Körber, Tania T. Sitz, Tobias Abdalla, Muna A. Mühling, Karl H. Rohn, Sascha |
author_facet | Körber, Tania T. Sitz, Tobias Abdalla, Muna A. Mühling, Karl H. Rohn, Sascha |
author_sort | Körber, Tania T. |
collection | PubMed |
description | Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were used to identify individual thylakoid membrane lipids, which might act as markers in S nutrition (especially under stress conditions). The thylakoid membrane consists of the three glycolipid classes: monogalactosyl- (MGDG), digalactosyl- (DGDG), and sulfoquinovosyl diacylglycerols (SQDG). All of them have two fatty acids linked, differing in chain length and degree of saturation. LC-ESI-MS/MS served as a powerful method to identify trends in the change in individual lipids and to understand strategies of the plant responding to stress. Being a good model plant, but also one of the most important fresh-cut vegetables in the world, lettuce (Lactuca sativa L.) has already been shown to respond significantly to different states of sulfur supply. The results showed a transformation of the glycolipids in lettuce plants and trends towards a higher degree of saturation of the lipids and an increased level of oxidized SQDG under S-limiting conditions. Changes in individual MGDG, DGDG, and oxidized SQDG were associated to S-related stress for the first time. Promisingly, oxidized SQDG might even serve as markers for further abiotic stress factors. |
format | Online Article Text |
id | pubmed-9965601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99656012023-02-26 LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition Körber, Tania T. Sitz, Tobias Abdalla, Muna A. Mühling, Karl H. Rohn, Sascha Int J Mol Sci Article Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were used to identify individual thylakoid membrane lipids, which might act as markers in S nutrition (especially under stress conditions). The thylakoid membrane consists of the three glycolipid classes: monogalactosyl- (MGDG), digalactosyl- (DGDG), and sulfoquinovosyl diacylglycerols (SQDG). All of them have two fatty acids linked, differing in chain length and degree of saturation. LC-ESI-MS/MS served as a powerful method to identify trends in the change in individual lipids and to understand strategies of the plant responding to stress. Being a good model plant, but also one of the most important fresh-cut vegetables in the world, lettuce (Lactuca sativa L.) has already been shown to respond significantly to different states of sulfur supply. The results showed a transformation of the glycolipids in lettuce plants and trends towards a higher degree of saturation of the lipids and an increased level of oxidized SQDG under S-limiting conditions. Changes in individual MGDG, DGDG, and oxidized SQDG were associated to S-related stress for the first time. Promisingly, oxidized SQDG might even serve as markers for further abiotic stress factors. MDPI 2023-02-13 /pmc/articles/PMC9965601/ /pubmed/36835138 http://dx.doi.org/10.3390/ijms24043728 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Körber, Tania T. Sitz, Tobias Abdalla, Muna A. Mühling, Karl H. Rohn, Sascha LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title | LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title_full | LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title_fullStr | LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title_full_unstemmed | LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title_short | LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition |
title_sort | lc-esi-ms/ms analysis of sulfolipids and galactolipids in green and red lettuce (lactuca sativa l.) as influenced by sulfur nutrition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965601/ https://www.ncbi.nlm.nih.gov/pubmed/36835138 http://dx.doi.org/10.3390/ijms24043728 |
work_keys_str_mv | AT korbertaniat lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition AT sitztobias lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition AT abdallamunaa lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition AT muhlingkarlh lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition AT rohnsascha lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition |