Cargando…

LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition

Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were...

Descripción completa

Detalles Bibliográficos
Autores principales: Körber, Tania T., Sitz, Tobias, Abdalla, Muna A., Mühling, Karl H., Rohn, Sascha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965601/
https://www.ncbi.nlm.nih.gov/pubmed/36835138
http://dx.doi.org/10.3390/ijms24043728
_version_ 1784896805723439104
author Körber, Tania T.
Sitz, Tobias
Abdalla, Muna A.
Mühling, Karl H.
Rohn, Sascha
author_facet Körber, Tania T.
Sitz, Tobias
Abdalla, Muna A.
Mühling, Karl H.
Rohn, Sascha
author_sort Körber, Tania T.
collection PubMed
description Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were used to identify individual thylakoid membrane lipids, which might act as markers in S nutrition (especially under stress conditions). The thylakoid membrane consists of the three glycolipid classes: monogalactosyl- (MGDG), digalactosyl- (DGDG), and sulfoquinovosyl diacylglycerols (SQDG). All of them have two fatty acids linked, differing in chain length and degree of saturation. LC-ESI-MS/MS served as a powerful method to identify trends in the change in individual lipids and to understand strategies of the plant responding to stress. Being a good model plant, but also one of the most important fresh-cut vegetables in the world, lettuce (Lactuca sativa L.) has already been shown to respond significantly to different states of sulfur supply. The results showed a transformation of the glycolipids in lettuce plants and trends towards a higher degree of saturation of the lipids and an increased level of oxidized SQDG under S-limiting conditions. Changes in individual MGDG, DGDG, and oxidized SQDG were associated to S-related stress for the first time. Promisingly, oxidized SQDG might even serve as markers for further abiotic stress factors.
format Online
Article
Text
id pubmed-9965601
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99656012023-02-26 LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition Körber, Tania T. Sitz, Tobias Abdalla, Muna A. Mühling, Karl H. Rohn, Sascha Int J Mol Sci Article Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were used to identify individual thylakoid membrane lipids, which might act as markers in S nutrition (especially under stress conditions). The thylakoid membrane consists of the three glycolipid classes: monogalactosyl- (MGDG), digalactosyl- (DGDG), and sulfoquinovosyl diacylglycerols (SQDG). All of them have two fatty acids linked, differing in chain length and degree of saturation. LC-ESI-MS/MS served as a powerful method to identify trends in the change in individual lipids and to understand strategies of the plant responding to stress. Being a good model plant, but also one of the most important fresh-cut vegetables in the world, lettuce (Lactuca sativa L.) has already been shown to respond significantly to different states of sulfur supply. The results showed a transformation of the glycolipids in lettuce plants and trends towards a higher degree of saturation of the lipids and an increased level of oxidized SQDG under S-limiting conditions. Changes in individual MGDG, DGDG, and oxidized SQDG were associated to S-related stress for the first time. Promisingly, oxidized SQDG might even serve as markers for further abiotic stress factors. MDPI 2023-02-13 /pmc/articles/PMC9965601/ /pubmed/36835138 http://dx.doi.org/10.3390/ijms24043728 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Körber, Tania T.
Sitz, Tobias
Abdalla, Muna A.
Mühling, Karl H.
Rohn, Sascha
LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title_full LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title_fullStr LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title_full_unstemmed LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title_short LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
title_sort lc-esi-ms/ms analysis of sulfolipids and galactolipids in green and red lettuce (lactuca sativa l.) as influenced by sulfur nutrition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965601/
https://www.ncbi.nlm.nih.gov/pubmed/36835138
http://dx.doi.org/10.3390/ijms24043728
work_keys_str_mv AT korbertaniat lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition
AT sitztobias lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition
AT abdallamunaa lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition
AT muhlingkarlh lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition
AT rohnsascha lcesimsmsanalysisofsulfolipidsandgalactolipidsingreenandredlettucelactucasativalasinfluencedbysulfurnutrition