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Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness
Chilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susce...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965640/ https://www.ncbi.nlm.nih.gov/pubmed/36840114 http://dx.doi.org/10.3390/plants12040766 |
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author | Olmedo, Patricio Zepeda, Baltasar Delgado-Rioseco, Joaquín Leiva, Carol Moreno, Adrián A. Sagredo, Karen Blanco-Herrera, Francisca Pedreschi, Romina Infante, Rodrigo Meneses, Claudio Campos-Vargas, Reinaldo |
author_facet | Olmedo, Patricio Zepeda, Baltasar Delgado-Rioseco, Joaquín Leiva, Carol Moreno, Adrián A. Sagredo, Karen Blanco-Herrera, Francisca Pedreschi, Romina Infante, Rodrigo Meneses, Claudio Campos-Vargas, Reinaldo |
author_sort | Olmedo, Patricio |
collection | PubMed |
description | Chilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susceptibility to mealiness after cold storage. A non-targeted metabolomic analysis was conducted by GC-MS to understand if changes in metabolite abundance are associated with nectarine mealiness induced by cold storage. Multivariate analyses indicated that in unripe nectarines, cold storage promoted a higher accumulation of amino acids in both varieties. Interestingly, for ripe nectarines, cold storage induced an accumulation of fewer amino acids in both varieties and showed an increased abundance of sugars and organic acids. A pathway reconstruction of primary metabolism revealed that in ripe nectarines, cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle, leading to a differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines. |
format | Online Article Text |
id | pubmed-9965640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99656402023-02-26 Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness Olmedo, Patricio Zepeda, Baltasar Delgado-Rioseco, Joaquín Leiva, Carol Moreno, Adrián A. Sagredo, Karen Blanco-Herrera, Francisca Pedreschi, Romina Infante, Rodrigo Meneses, Claudio Campos-Vargas, Reinaldo Plants (Basel) Article Chilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susceptibility to mealiness after cold storage. A non-targeted metabolomic analysis was conducted by GC-MS to understand if changes in metabolite abundance are associated with nectarine mealiness induced by cold storage. Multivariate analyses indicated that in unripe nectarines, cold storage promoted a higher accumulation of amino acids in both varieties. Interestingly, for ripe nectarines, cold storage induced an accumulation of fewer amino acids in both varieties and showed an increased abundance of sugars and organic acids. A pathway reconstruction of primary metabolism revealed that in ripe nectarines, cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle, leading to a differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines. MDPI 2023-02-08 /pmc/articles/PMC9965640/ /pubmed/36840114 http://dx.doi.org/10.3390/plants12040766 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Olmedo, Patricio Zepeda, Baltasar Delgado-Rioseco, Joaquín Leiva, Carol Moreno, Adrián A. Sagredo, Karen Blanco-Herrera, Francisca Pedreschi, Romina Infante, Rodrigo Meneses, Claudio Campos-Vargas, Reinaldo Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title | Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title_full | Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title_fullStr | Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title_full_unstemmed | Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title_short | Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness |
title_sort | metabolite profiling reveals the effect of cold storage on primary metabolism in nectarine varieties with contrasting mealiness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965640/ https://www.ncbi.nlm.nih.gov/pubmed/36840114 http://dx.doi.org/10.3390/plants12040766 |
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