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Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate
Bone marrow is complex structure containing heterogenetic cells, making it difficult to regenerate using artificial scaffolds. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which is a cylindrical scaffold with a honeycomb arrangement of straight pores, and we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965701/ https://www.ncbi.nlm.nih.gov/pubmed/36837023 http://dx.doi.org/10.3390/ma16041393 |
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author | Inada, Yasunori Takabatake, Kiyofumi Tsujigiwa, Hidetsugu Nakano, Keisuke Shan, Qiusheng Piao, Tianyan Chang, Anqi Kawai, Hotaka Nagatsuka, Hitoshi |
author_facet | Inada, Yasunori Takabatake, Kiyofumi Tsujigiwa, Hidetsugu Nakano, Keisuke Shan, Qiusheng Piao, Tianyan Chang, Anqi Kawai, Hotaka Nagatsuka, Hitoshi |
author_sort | Inada, Yasunori |
collection | PubMed |
description | Bone marrow is complex structure containing heterogenetic cells, making it difficult to regenerate using artificial scaffolds. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which is a cylindrical scaffold with a honeycomb arrangement of straight pores, and we demonstrated that TCP with 300 and 500 μm pore diameters (300TCP and 500TCP) induced bone marrow structure within the pores. In this study, we examined the optimal scaffold structure for bone marrow with homeostatic bone metabolism using honeycomb TCP. 300TCP and 500TCP were transplanted into rat muscle, and bone marrow formation was histologically assessed. Immunohistochemistry for CD45, CD34, Runt-related transcription factor 2 (Runx2), c-kit single staining, Runx2/N-cadherin, and c-kit/Tie-2 double staining was performed. The area of bone marrow structure, which includes CD45(+) round-shaped hematopoietic cells and CD34(+) sinusoidal vessels, was larger in 300TCP than in 500TCP. Additionally, Runx2(+) osteoblasts and c-kit(+) hematopoietic stem cells were observed on the surface of bone tissue formed within TCP. Among Runx2(+) osteoblasts, spindle-shaped N-cadherin(+) cells existed in association with c-kit(+)Tie-2(+) hematopoietic stem cells on the bone tissue formed within TCP, which formed a hematopoietic stem cell niche similar to as in vivo. Therefore, honeycomb TCP with 300 μm pore diameters may be an artificial scaffold with an optimal geometric structure as a scaffold for bone marrow formation. |
format | Online Article Text |
id | pubmed-9965701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99657012023-02-26 Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate Inada, Yasunori Takabatake, Kiyofumi Tsujigiwa, Hidetsugu Nakano, Keisuke Shan, Qiusheng Piao, Tianyan Chang, Anqi Kawai, Hotaka Nagatsuka, Hitoshi Materials (Basel) Article Bone marrow is complex structure containing heterogenetic cells, making it difficult to regenerate using artificial scaffolds. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which is a cylindrical scaffold with a honeycomb arrangement of straight pores, and we demonstrated that TCP with 300 and 500 μm pore diameters (300TCP and 500TCP) induced bone marrow structure within the pores. In this study, we examined the optimal scaffold structure for bone marrow with homeostatic bone metabolism using honeycomb TCP. 300TCP and 500TCP were transplanted into rat muscle, and bone marrow formation was histologically assessed. Immunohistochemistry for CD45, CD34, Runt-related transcription factor 2 (Runx2), c-kit single staining, Runx2/N-cadherin, and c-kit/Tie-2 double staining was performed. The area of bone marrow structure, which includes CD45(+) round-shaped hematopoietic cells and CD34(+) sinusoidal vessels, was larger in 300TCP than in 500TCP. Additionally, Runx2(+) osteoblasts and c-kit(+) hematopoietic stem cells were observed on the surface of bone tissue formed within TCP. Among Runx2(+) osteoblasts, spindle-shaped N-cadherin(+) cells existed in association with c-kit(+)Tie-2(+) hematopoietic stem cells on the bone tissue formed within TCP, which formed a hematopoietic stem cell niche similar to as in vivo. Therefore, honeycomb TCP with 300 μm pore diameters may be an artificial scaffold with an optimal geometric structure as a scaffold for bone marrow formation. MDPI 2023-02-07 /pmc/articles/PMC9965701/ /pubmed/36837023 http://dx.doi.org/10.3390/ma16041393 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Inada, Yasunori Takabatake, Kiyofumi Tsujigiwa, Hidetsugu Nakano, Keisuke Shan, Qiusheng Piao, Tianyan Chang, Anqi Kawai, Hotaka Nagatsuka, Hitoshi Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title | Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title_full | Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title_fullStr | Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title_full_unstemmed | Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title_short | Novel Artificial Scaffold for Bone Marrow Regeneration: Honeycomb Tricalcium Phosphate |
title_sort | novel artificial scaffold for bone marrow regeneration: honeycomb tricalcium phosphate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965701/ https://www.ncbi.nlm.nih.gov/pubmed/36837023 http://dx.doi.org/10.3390/ma16041393 |
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