Cargando…

Optimization of Primary Human Bronchial Epithelial 3D Cell Culture with Donor-Matched Fibroblasts and Comparison of Two Different Culture Media

In vitro airway models are increasingly important for pathomechanistic analyses of respiratory diseases. Existing models are limited in their validity by their incomplete cellular complexity. We therefore aimed to generate a more complex and meaningful three-dimensional (3D) airway model. Primary hu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Maurer, Julian, Walles, Thorsten, Wiese-Rischke, Cornelia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965758/ https://www.ncbi.nlm.nih.gov/pubmed/36835529 http://dx.doi.org/10.3390/ijms24044113

Ejemplares similares

Biological Models of the Lower Human Airways—Challenges and Special Requirements of Human 3D Barrier Models for Biomedical Research
por: Wiese-Rischke, Cornelia, et al.
Publicado: (2021)

Equine bronchial fibroblasts enhance proliferation and differentiation of primary equine bronchial epithelial cells co-cultured under air-liquid interface
por: Abs, Vanessa, et al.
Publicado: (2019)

Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading
por: Möckel, Marion, et al.
Publicado: (2022)

Susceptibility of primary human airway epithelial cells to Bordetella pertussis adenylate cyclase toxin in two- and three-dimensional culture conditions
por: Bianchi, Maria, et al.
Publicado: (2020)

Effects of ozone stimulation of bronchial epithelial cells on proliferation and collagen synthesis of co-cultured lung fibroblasts
por: Wang, Yue, et al.
Publicado: (2018)

In vitro skin culture media influence the viability and inflammatory response of primary macrophages
por: Griffoni, Chiara, et al.
Publicado: (2021)

Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
por: Iskandar, Anita R., et al.
Publicado: (2015)

Fibroblast gene expression following asthmatic bronchial epithelial cell conditioning correlates with epithelial donor lung function and exacerbation history
por: Reeves, Stephen R., et al.
Publicado: (2018)

Optimized Protocol for Isolation and Culture of Murine Neonatal Primary Lung Fibroblasts
por: Fuentes-Mateos, Rocío, et al.
Publicado: (2023)

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies
por: Rayner, Rachael E., et al.
Publicado: (2019)

Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research
por: Stewart, Ceri E., et al.
Publicado: (2012)

MIXED CULTURES OF PURE STRAINS OF FIBROBLASTS AND EPITHELIAL CELLS
por: Ebeling, Albert H., et al.
Publicado: (1922)

Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture
por: Lodes, Nina, et al.
Publicado: (2020)

The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD
por: Bostancieri, Nuray, et al.
Publicado: (2023)

The Effects on Bronchial Epithelial Mucociliary Cultures of Coarse, Fine, and Ultrafine Particulate Matter From an Underground Railway Station
por: Loxham, Matthew, et al.
Publicado: (2015)

Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions
por: Abraham, Getu, et al.
Publicado: (2011)

Bronchial epithelial spheroids: an alternative culture model to investigate epithelium inflammation-mediated COPD
por: Deslee, Gaetan, et al.
Publicado: (2007)

Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
por: Gabrielson, E W, et al.
Publicado: (1994)

Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media
por: Broadbent, Lindsay, et al.
Publicado: (2020)

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells
por: Pathak, Juhi, et al.
Publicado: (2023)

Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model
por: Callaghan, Patrick J., et al.
Publicado: (2020)

Co-Culture of Human Bronchial Fibroblasts and CD4+ T Cells Increases Th17 Cytokine Signature
por: Loubaki, Lionel, et al.
Publicado: (2013)

Different culture media modulate growth, heterogeneity, and senescence in human mammary epithelial cell cultures
por: Lee, Jonathan K., et al.
Publicado: (2018)

The larger attachment glycoprotein of respiratory syncytial virus produced in primary human bronchial epithelial cultures reduces infectivity for cell lines
por: King, Tiffany, et al.
Publicado: (2021)

Liquid Application Dosing Alters the Physiology of Air-Liquid Interface Primary Bronchial Epithelial Cultures and In vitro Testing Relevant Endpoints
por: Mallek, Nicholas M., et al.
Publicado: (2023)

Human rhinovirus infection of human bronchial epithelial cells results in migration of human bronchial fibroblast cells
por: Shelfoon, Christopher, et al.
Publicado: (2014)

Azithromycin induces anti-viral effects in cultured bronchial epithelial cells from COPD patients
por: Menzel, Mandy, et al.
Publicado: (2016)

Shoseiryuto Promotes the Formation of a Tight-Junction Barrier in Cultured Human Bronchial Epithelial Cells
por: Lu, Jingya, et al.
Publicado: (2023)

A molecular signature of epithelial host defense: comparative gene expression analysis of cultured bronchial epithelial cells and keratinocytes
por: Vos, Joost B, et al.
Publicado: (2006)

Reaction of mineral dusts with primary lung fibroblast cultures.
por: Richards, R J, et al.
Publicado: (1983)

Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures
por: Chaudhry, Imran S., et al.
Publicado: (2011)

Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells
por: ter Ellen, Bram M., et al.
Publicado: (2021)

Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts
por: Kawecki, Fabien, et al.
Publicado: (2022)

Responsiveness of human bronchial fibroblasts and epithelial cells from asthmatic and non-asthmatic donors to the transforming growth factor-β(1) in epithelial-mesenchymal trophic unit model
por: Paw, Milena, et al.
Publicado: (2021)

Increased levels of circulating autoantibodies to cultured human bronchial epithelial cell in adult patients with nonatopic asthma.
por: Nahm, D. H., et al.
Publicado: (2001)

Organotypic sinonasal airway culture systems are predictive of the mucociliary phenotype produced by bronchial airway epithelial cells
por: Delhove, Juliette, et al.
Publicado: (2022)

Capsaicin suppresses ciliary function, while inducing permeability in bronchial epithelial cell cultures of COPD patients
por: Toy, Halil Ibrahim, et al.
Publicado: (2022)

The impact of amphotericin B-fortified preservation media on donor rim cultures and posttransplant infection
por: Hussey, Vincent, et al.
Publicado: (2022)

A review of algorithmic approaches for cell culture media optimization
por: Zhou, Tianxun, et al.
Publicado: (2023)

Optimizing a 3D Culture System to Study the Interaction between Epithelial Breast Cancer and Its Surrounding Fibroblasts
por: Li, Liyuan, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_10vk7iukic15rto49pcjjst942