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Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis

In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbit...

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Autores principales: Rakk, Dávid, Kukolya, József, Škrbić, Biljana D., Vágvölgyi, Csaba, Varga, Mónika, Szekeres, András
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965799/
https://www.ncbi.nlm.nih.gov/pubmed/36828448
http://dx.doi.org/10.3390/toxins15020134
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author Rakk, Dávid
Kukolya, József
Škrbić, Biljana D.
Vágvölgyi, Csaba
Varga, Mónika
Szekeres, András
author_facet Rakk, Dávid
Kukolya, József
Škrbić, Biljana D.
Vágvölgyi, Csaba
Varga, Mónika
Szekeres, András
author_sort Rakk, Dávid
collection PubMed
description In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbitrap analyzators, can provide stable instrumental background for high-throughput analyses. In this report, a short, 1 min MS-based multi-mycotoxin method was developed with the application of a short column as a reduced chromatographic separation, taking advantages of the multiplexing and high-resolution capability of the QExactive Orbitrap MS possessing sub-1 ppm mass accuracy. The performance of the method was evaluated regarding selectivity, LOD, LOQ, linearity, matrix effect, and recovery, and compared to a UHPLC-MS/MS method. The final multiplexing method was able to quantify 11 mycotoxins in defined ranges (aflatoxins (corn, 2.8–600 μg/kg; wheat, 1.5–350 μg/kg), deoxynivalenol (corn, 640–9600 μg/kg; wheat, 128–3500 μg/kg), fumonisins (corn, 20–1500 μg/kg; wheat, 30–3500 μg/kg), HT-2 (corn, 64–5200 μg/kg; wheat, 61–3500 μg/kg), T-2 (corn, 10–800 μg/kg; wheat, 4–250 μg/kg), ochratoxin (corn, 4.7–600 μg/kg; wheat, 1–1000 μg/kg), zearalenone (corn, 64–4800 μg/kg; wheat, 4–500 μg/kg)) within one minute in corn and wheat matrices at the MRL levels stated by the European Union.
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spelling pubmed-99657992023-02-26 Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis Rakk, Dávid Kukolya, József Škrbić, Biljana D. Vágvölgyi, Csaba Varga, Mónika Szekeres, András Toxins (Basel) Article In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbitrap analyzators, can provide stable instrumental background for high-throughput analyses. In this report, a short, 1 min MS-based multi-mycotoxin method was developed with the application of a short column as a reduced chromatographic separation, taking advantages of the multiplexing and high-resolution capability of the QExactive Orbitrap MS possessing sub-1 ppm mass accuracy. The performance of the method was evaluated regarding selectivity, LOD, LOQ, linearity, matrix effect, and recovery, and compared to a UHPLC-MS/MS method. The final multiplexing method was able to quantify 11 mycotoxins in defined ranges (aflatoxins (corn, 2.8–600 μg/kg; wheat, 1.5–350 μg/kg), deoxynivalenol (corn, 640–9600 μg/kg; wheat, 128–3500 μg/kg), fumonisins (corn, 20–1500 μg/kg; wheat, 30–3500 μg/kg), HT-2 (corn, 64–5200 μg/kg; wheat, 61–3500 μg/kg), T-2 (corn, 10–800 μg/kg; wheat, 4–250 μg/kg), ochratoxin (corn, 4.7–600 μg/kg; wheat, 1–1000 μg/kg), zearalenone (corn, 64–4800 μg/kg; wheat, 4–500 μg/kg)) within one minute in corn and wheat matrices at the MRL levels stated by the European Union. MDPI 2023-02-07 /pmc/articles/PMC9965799/ /pubmed/36828448 http://dx.doi.org/10.3390/toxins15020134 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rakk, Dávid
Kukolya, József
Škrbić, Biljana D.
Vágvölgyi, Csaba
Varga, Mónika
Szekeres, András
Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title_full Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title_fullStr Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title_full_unstemmed Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title_short Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
title_sort advantages of multiplexing ability of the orbitrap mass analyzer in the multi-mycotoxin analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965799/
https://www.ncbi.nlm.nih.gov/pubmed/36828448
http://dx.doi.org/10.3390/toxins15020134
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