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Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection

Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs and exosomes in particular have the natural ability to transport nucleic acids, proteins, and other components of cellular or viral origi...

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Autores principales: Safadi, Daed El, Lebeau, Grégorie, Lagrave, Alisé, Mélade, Julien, Grondin, Lauriane, Rosanaly, Sarah, Begue, Floran, Hoareau, Mathilde, Veeren, Bryan, Roche, Marjolaine, Hoarau, Jean-Jacques, Meilhac, Olivier, Mavingui, Patrick, Desprès, Philippe, Viranaïcken, Wildriss, Krejbich-Trotot, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965858/
https://www.ncbi.nlm.nih.gov/pubmed/36851578
http://dx.doi.org/10.3390/v15020364
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author Safadi, Daed El
Lebeau, Grégorie
Lagrave, Alisé
Mélade, Julien
Grondin, Lauriane
Rosanaly, Sarah
Begue, Floran
Hoareau, Mathilde
Veeren, Bryan
Roche, Marjolaine
Hoarau, Jean-Jacques
Meilhac, Olivier
Mavingui, Patrick
Desprès, Philippe
Viranaïcken, Wildriss
Krejbich-Trotot, Pascale
author_facet Safadi, Daed El
Lebeau, Grégorie
Lagrave, Alisé
Mélade, Julien
Grondin, Lauriane
Rosanaly, Sarah
Begue, Floran
Hoareau, Mathilde
Veeren, Bryan
Roche, Marjolaine
Hoarau, Jean-Jacques
Meilhac, Olivier
Mavingui, Patrick
Desprès, Philippe
Viranaïcken, Wildriss
Krejbich-Trotot, Pascale
author_sort Safadi, Daed El
collection PubMed
description Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs and exosomes in particular have the natural ability to transport nucleic acids, proteins, and other components of cellular or viral origin. Thus, they participate in intercellular communication, immune responses, and infectious and pathophysiological processes. Some viruses are known to hijack the cell production and content of EVs for their benefit. Here, we investigate whether two pathogenic flaviviruses i.e., Zika Virus (ZIKV) and Dengue virus (DENV2) could have an impact on the features of EVs. The analysis of EVs produced by infected cells allowed us to identify that the non-structural protein 1 (NS1), described as a viral toxin, is associated with exosomes. This observation could be confirmed under conditions of overexpression of recombinant NS1 from each flavivirus. Using different isolation methods (i.e., exosome isolation kit, size exclusion chromatography, Polyethylene Glycol enrichment, and ELISA capture), we showed that NS1 was present as a dimer at the surface of excreted exosomes, and that this association could occur in the extracellular compartment. This finding could be of major importance in a physiological context. Indeed, this capacity of NS1 to address EVs and its implication in the pathophysiology during Dengue or Zika diseases should be explored. Furthermore, exosomes that have demonstrated a natural capacity to vectorize NS1 could serve as useful tools for vaccine development.
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spelling pubmed-99658582023-02-26 Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection Safadi, Daed El Lebeau, Grégorie Lagrave, Alisé Mélade, Julien Grondin, Lauriane Rosanaly, Sarah Begue, Floran Hoareau, Mathilde Veeren, Bryan Roche, Marjolaine Hoarau, Jean-Jacques Meilhac, Olivier Mavingui, Patrick Desprès, Philippe Viranaïcken, Wildriss Krejbich-Trotot, Pascale Viruses Article Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs and exosomes in particular have the natural ability to transport nucleic acids, proteins, and other components of cellular or viral origin. Thus, they participate in intercellular communication, immune responses, and infectious and pathophysiological processes. Some viruses are known to hijack the cell production and content of EVs for their benefit. Here, we investigate whether two pathogenic flaviviruses i.e., Zika Virus (ZIKV) and Dengue virus (DENV2) could have an impact on the features of EVs. The analysis of EVs produced by infected cells allowed us to identify that the non-structural protein 1 (NS1), described as a viral toxin, is associated with exosomes. This observation could be confirmed under conditions of overexpression of recombinant NS1 from each flavivirus. Using different isolation methods (i.e., exosome isolation kit, size exclusion chromatography, Polyethylene Glycol enrichment, and ELISA capture), we showed that NS1 was present as a dimer at the surface of excreted exosomes, and that this association could occur in the extracellular compartment. This finding could be of major importance in a physiological context. Indeed, this capacity of NS1 to address EVs and its implication in the pathophysiology during Dengue or Zika diseases should be explored. Furthermore, exosomes that have demonstrated a natural capacity to vectorize NS1 could serve as useful tools for vaccine development. MDPI 2023-01-27 /pmc/articles/PMC9965858/ /pubmed/36851578 http://dx.doi.org/10.3390/v15020364 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Safadi, Daed El
Lebeau, Grégorie
Lagrave, Alisé
Mélade, Julien
Grondin, Lauriane
Rosanaly, Sarah
Begue, Floran
Hoareau, Mathilde
Veeren, Bryan
Roche, Marjolaine
Hoarau, Jean-Jacques
Meilhac, Olivier
Mavingui, Patrick
Desprès, Philippe
Viranaïcken, Wildriss
Krejbich-Trotot, Pascale
Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title_full Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title_fullStr Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title_full_unstemmed Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title_short Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
title_sort extracellular vesicles are conveyors of the ns1 toxin during dengue virus and zika virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965858/
https://www.ncbi.nlm.nih.gov/pubmed/36851578
http://dx.doi.org/10.3390/v15020364
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